Experiment: ICP1_phage_0.23_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_00440 and CSW01_00445 are separated by 44 nucleotides CSW01_00445 and CSW01_00450 are separated by 8 nucleotides CSW01_00450 and CSW01_00455 overlap by 4 nucleotides
CSW01_00440: CSW01_00440 - DNA recombination protein RmuC, at 80,087 to 81,619
_00440
CSW01_00445: CSW01_00445 - ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 81,664 to 82,446
_00445
CSW01_00450: CSW01_00450 - SCP2 domain-containing protein, at 82,455 to 83,078
_00450
CSW01_00455: CSW01_00455 - ubiquinone biosynthesis protein UbiB, at 83,075 to 84,709
_00455
Position (kb) 82
83
84 Strain fitness (log2 ratio) -2
-1
0
1 at 81.456 kb on - strand, within CSW01_00440 at 81.489 kb on + strand at 81.497 kb on - strand at 81.497 kb on - strand at 81.532 kb on + strand at 81.559 kb on - strand at 81.592 kb on - strand at 82.526 kb on + strand, within CSW01_00450 at 82.844 kb on + strand, within CSW01_00450 at 82.872 kb on + strand, within CSW01_00450 at 82.872 kb on + strand, within CSW01_00450 at 83.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep1 remove 81,456 - CSW01_00440 0.89 +1.4 81,489 + -0.8 81,497 - +0.6 81,497 - -2.2 81,532 + -0.3 81,559 - -0.4 81,592 - -0.1 82,526 + CSW01_00450 0.11 -0.4 82,844 + CSW01_00450 0.62 -1.8 82,872 + CSW01_00450 0.67 -1.0 82,872 + CSW01_00450 0.67 -2.4 83,089 + +0.0
Or see this region's nucleotide sequence