Experiment: Temperature shift 10_to_34; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01785 and PFLU_RS01790 are separated by 71 nucleotides PFLU_RS01790 and PFLU_RS01795 overlap by 4 nucleotides PFLU_RS01795 and PFLU_RS01800 are separated by 143 nucleotides
PFLU_RS01785: PFLU_RS01785 - ABC transporter substrate-binding protein, at 401,096 to 402,061
_RS01785
PFLU_RS01790: PFLU_RS01790 - proline/glycine betaine ABC transporter permease, at 402,133 to 402,984
_RS01790
PFLU_RS01795: PFLU_RS01795 - glycine betaine/L-proline ABC transporter ATP-binding protein, at 402,981 to 403,805
_RS01795
PFLU_RS01800: PFLU_RS01800 - histidine ammonia-lyase, at 403,949 to 405,457
_RS01800
Position (kb) 402
403 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.271 kb on - strand, within PFLU_RS01785 at 401.453 kb on + strand, within PFLU_RS01785 at 401.453 kb on + strand, within PFLU_RS01785 at 401.453 kb on + strand, within PFLU_RS01785 at 401.453 kb on + strand, within PFLU_RS01785 at 401.454 kb on - strand, within PFLU_RS01785 at 401.454 kb on - strand, within PFLU_RS01785 at 401.454 kb on - strand, within PFLU_RS01785 at 401.454 kb on - strand, within PFLU_RS01785 at 401.465 kb on + strand, within PFLU_RS01785 at 401.465 kb on + strand, within PFLU_RS01785 at 401.466 kb on - strand, within PFLU_RS01785 at 401.466 kb on - strand, within PFLU_RS01785 at 401.543 kb on + strand, within PFLU_RS01785 at 401.543 kb on + strand, within PFLU_RS01785 at 401.543 kb on + strand, within PFLU_RS01785 at 401.544 kb on - strand, within PFLU_RS01785 at 401.544 kb on - strand, within PFLU_RS01785 at 401.544 kb on - strand, within PFLU_RS01785 at 401.544 kb on - strand, within PFLU_RS01785 at 401.591 kb on + strand, within PFLU_RS01785 at 401.591 kb on + strand, within PFLU_RS01785 at 401.591 kb on + strand, within PFLU_RS01785 at 401.592 kb on - strand, within PFLU_RS01785 at 401.592 kb on - strand, within PFLU_RS01785 at 401.592 kb on - strand, within PFLU_RS01785 at 401.592 kb on - strand, within PFLU_RS01785 at 401.648 kb on + strand, within PFLU_RS01785 at 401.649 kb on - strand, within PFLU_RS01785 at 401.649 kb on - strand, within PFLU_RS01785 at 401.649 kb on - strand, within PFLU_RS01785 at 401.649 kb on - strand, within PFLU_RS01785 at 401.666 kb on + strand, within PFLU_RS01785 at 401.666 kb on + strand, within PFLU_RS01785 at 401.666 kb on + strand, within PFLU_RS01785 at 401.666 kb on + strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.667 kb on - strand, within PFLU_RS01785 at 401.723 kb on + strand, within PFLU_RS01785 at 401.724 kb on - strand, within PFLU_RS01785 at 401.750 kb on + strand, within PFLU_RS01785 at 401.750 kb on + strand, within PFLU_RS01785 at 401.750 kb on + strand, within PFLU_RS01785 at 401.751 kb on - strand, within PFLU_RS01785 at 401.751 kb on - strand, within PFLU_RS01785 at 401.751 kb on - strand, within PFLU_RS01785 at 401.751 kb on - strand, within PFLU_RS01785 at 401.751 kb on - strand, within PFLU_RS01785 at 401.753 kb on + strand, within PFLU_RS01785 at 401.753 kb on + strand, within PFLU_RS01785 at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.059 kb on + strand at 402.060 kb on - strand at 402.060 kb on - strand at 402.060 kb on - strand at 402.060 kb on - strand at 402.060 kb on - strand at 402.060 kb on - strand at 402.132 kb on + strand at 402.132 kb on + strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.133 kb on - strand at 402.150 kb on + strand at 402.150 kb on + strand at 402.150 kb on + strand at 402.151 kb on - strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.205 kb on + strand at 402.206 kb on - strand at 402.206 kb on - strand at 402.206 kb on - strand at 402.206 kb on - strand at 402.206 kb on - strand at 402.206 kb on - strand at 402.242 kb on + strand, within PFLU_RS01790 at 402.242 kb on + strand, within PFLU_RS01790 at 402.243 kb on - strand, within PFLU_RS01790 at 402.557 kb on - strand, within PFLU_RS01790 at 402.557 kb on - strand, within PFLU_RS01790 at 402.622 kb on + strand, within PFLU_RS01790 at 402.622 kb on + strand, within PFLU_RS01790 at 402.622 kb on + strand, within PFLU_RS01790 at 402.623 kb on - strand, within PFLU_RS01790 at 402.854 kb on + strand, within PFLU_RS01790 at 402.854 kb on + strand, within PFLU_RS01790 at 402.854 kb on + strand, within PFLU_RS01790 at 402.855 kb on - strand, within PFLU_RS01790 at 402.855 kb on - strand, within PFLU_RS01790 at 402.855 kb on - strand, within PFLU_RS01790 at 402.855 kb on - strand, within PFLU_RS01790 at 402.855 kb on - strand, within PFLU_RS01790 at 402.952 kb on + strand at 402.952 kb on + strand at 402.952 kb on + strand at 402.952 kb on + strand at 402.952 kb on + strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.953 kb on - strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.987 kb on - strand at 402.995 kb on + strand at 402.996 kb on - strand at 403.007 kb on + strand at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.642 kb on + strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.643 kb on - strand, within PFLU_RS01795 at 403.646 kb on + strand, within PFLU_RS01795 at 403.646 kb on + strand, within PFLU_RS01795 at 403.646 kb on + strand, within PFLU_RS01795 at 403.647 kb on - strand, within PFLU_RS01795 at 403.647 kb on - strand, within PFLU_RS01795 at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.764 kb on + strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.765 kb on - strand at 403.872 kb on - strand at 403.942 kb on + strand at 403.942 kb on + strand at 403.942 kb on + strand at 403.943 kb on - strand at 403.943 kb on - strand at 403.943 kb on - strand at 403.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_34; with MOPS remove 401,271 - PFLU_RS01785 0.18 +0.5 401,453 + PFLU_RS01785 0.37 -0.5 401,453 + PFLU_RS01785 0.37 +1.8 401,453 + PFLU_RS01785 0.37 +0.8 401,453 + PFLU_RS01785 0.37 +0.1 401,454 - PFLU_RS01785 0.37 -0.4 401,454 - PFLU_RS01785 0.37 +1.3 401,454 - PFLU_RS01785 0.37 -1.1 401,454 - PFLU_RS01785 0.37 -1.6 401,465 + PFLU_RS01785 0.38 -0.6 401,465 + PFLU_RS01785 0.38 +0.4 401,466 - PFLU_RS01785 0.38 -0.0 401,466 - PFLU_RS01785 0.38 +1.0 401,543 + PFLU_RS01785 0.46 +0.4 401,543 + PFLU_RS01785 0.46 +0.4 401,543 + PFLU_RS01785 0.46 +0.4 401,544 - PFLU_RS01785 0.46 -0.1 401,544 - PFLU_RS01785 0.46 +0.5 401,544 - PFLU_RS01785 0.46 +1.3 401,544 - PFLU_RS01785 0.46 +1.0 401,591 + PFLU_RS01785 0.51 +1.6 401,591 + PFLU_RS01785 0.51 +0.4 401,591 + PFLU_RS01785 0.51 +0.3 401,592 - PFLU_RS01785 0.51 +1.4 401,592 - PFLU_RS01785 0.51 -0.4 401,592 - PFLU_RS01785 0.51 -0.0 401,592 - PFLU_RS01785 0.51 +0.3 401,648 + PFLU_RS01785 0.57 -0.6 401,649 - PFLU_RS01785 0.57 -0.9 401,649 - PFLU_RS01785 0.57 -1.0 401,649 - PFLU_RS01785 0.57 +1.2 401,649 - PFLU_RS01785 0.57 +0.5 401,666 + PFLU_RS01785 0.59 +0.8 401,666 + PFLU_RS01785 0.59 -0.9 401,666 + PFLU_RS01785 0.59 +0.2 401,666 + PFLU_RS01785 0.59 -1.1 401,667 - PFLU_RS01785 0.59 +2.5 401,667 - PFLU_RS01785 0.59 +0.4 401,667 - PFLU_RS01785 0.59 +0.5 401,667 - PFLU_RS01785 0.59 +1.5 401,667 - PFLU_RS01785 0.59 +0.6 401,667 - PFLU_RS01785 0.59 -0.9 401,667 - PFLU_RS01785 0.59 -0.6 401,667 - PFLU_RS01785 0.59 -0.6 401,723 + PFLU_RS01785 0.65 +0.7 401,724 - PFLU_RS01785 0.65 -0.2 401,750 + PFLU_RS01785 0.68 +0.5 401,750 + PFLU_RS01785 0.68 -0.9 401,750 + PFLU_RS01785 0.68 +0.2 401,751 - PFLU_RS01785 0.68 -1.5 401,751 - PFLU_RS01785 0.68 +2.0 401,751 - PFLU_RS01785 0.68 -0.6 401,751 - PFLU_RS01785 0.68 -0.7 401,751 - PFLU_RS01785 0.68 +0.4 401,753 + PFLU_RS01785 0.68 -0.1 401,753 + PFLU_RS01785 0.68 -0.1 402,059 + +0.1 402,059 + +1.5 402,059 + -0.3 402,059 + +0.6 402,059 + -2.0 402,059 + +2.0 402,059 + -1.7 402,059 + -1.0 402,059 + -0.0 402,059 + -0.8 402,060 - +0.4 402,060 - +0.4 402,060 - +0.8 402,060 - -1.4 402,060 - -0.4 402,060 - +0.7 402,132 + -1.3 402,132 + +1.1 402,133 - +1.2 402,133 - +0.3 402,133 - +0.9 402,133 - -2.1 402,133 - -0.6 402,133 - +1.2 402,133 - -0.1 402,133 - -1.4 402,133 - -1.5 402,150 + -0.5 402,150 + +0.4 402,150 + +0.7 402,151 - +0.9 402,205 + -0.4 402,205 + +0.0 402,205 + +0.1 402,205 + -0.2 402,205 + +1.4 402,205 + -1.3 402,205 + +1.0 402,205 + +0.3 402,205 + +0.7 402,206 - -1.5 402,206 - +1.0 402,206 - +0.6 402,206 - -0.3 402,206 - +0.1 402,206 - +0.5 402,242 + PFLU_RS01790 0.13 -0.7 402,242 + PFLU_RS01790 0.13 -1.3 402,243 - PFLU_RS01790 0.13 +0.1 402,557 - PFLU_RS01790 0.50 +0.4 402,557 - PFLU_RS01790 0.50 +1.0 402,622 + PFLU_RS01790 0.57 -1.5 402,622 + PFLU_RS01790 0.57 -0.4 402,622 + PFLU_RS01790 0.57 -1.5 402,623 - PFLU_RS01790 0.58 -0.9 402,854 + PFLU_RS01790 0.85 -0.8 402,854 + PFLU_RS01790 0.85 -0.5 402,854 + PFLU_RS01790 0.85 +0.4 402,855 - PFLU_RS01790 0.85 +0.9 402,855 - PFLU_RS01790 0.85 +0.6 402,855 - PFLU_RS01790 0.85 +0.4 402,855 - PFLU_RS01790 0.85 -1.2 402,855 - PFLU_RS01790 0.85 +0.2 402,952 + +0.8 402,952 + +1.9 402,952 + +0.4 402,952 + -1.8 402,952 + -0.4 402,953 - +0.9 402,953 - -0.5 402,953 - +0.8 402,953 - +0.6 402,953 - +0.9 402,953 - +0.3 402,953 - -0.7 402,953 - -1.2 402,953 - +0.2 402,953 - -2.5 402,953 - +0.5 402,986 + -1.2 402,986 + -1.5 402,986 + +0.1 402,986 + +1.0 402,986 + +1.0 402,986 + -0.4 402,986 + +0.6 402,986 + -0.1 402,987 - +0.4 402,987 - -1.9 402,987 - -0.2 402,987 - +0.8 402,987 - -0.9 402,987 - -0.9 402,987 - +0.3 402,995 + +0.8 402,996 - -0.5 403,007 + +0.2 403,642 + PFLU_RS01795 0.80 +1.3 403,642 + PFLU_RS01795 0.80 +2.0 403,642 + PFLU_RS01795 0.80 -0.6 403,642 + PFLU_RS01795 0.80 -1.2 403,642 + PFLU_RS01795 0.80 +0.4 403,642 + PFLU_RS01795 0.80 -1.1 403,642 + PFLU_RS01795 0.80 +0.0 403,642 + PFLU_RS01795 0.80 +0.2 403,642 + PFLU_RS01795 0.80 -0.4 403,642 + PFLU_RS01795 0.80 +0.2 403,642 + PFLU_RS01795 0.80 -0.4 403,642 + PFLU_RS01795 0.80 -1.7 403,642 + PFLU_RS01795 0.80 -0.4 403,643 - PFLU_RS01795 0.80 +0.5 403,643 - PFLU_RS01795 0.80 -1.2 403,643 - PFLU_RS01795 0.80 -1.1 403,643 - PFLU_RS01795 0.80 +0.6 403,643 - PFLU_RS01795 0.80 -1.0 403,643 - PFLU_RS01795 0.80 -0.6 403,643 - PFLU_RS01795 0.80 -0.9 403,643 - PFLU_RS01795 0.80 +0.3 403,643 - PFLU_RS01795 0.80 +0.2 403,643 - PFLU_RS01795 0.80 +1.8 403,643 - PFLU_RS01795 0.80 +0.1 403,646 + PFLU_RS01795 0.81 -0.7 403,646 + PFLU_RS01795 0.81 -0.1 403,646 + PFLU_RS01795 0.81 -0.3 403,647 - PFLU_RS01795 0.81 +0.8 403,647 - PFLU_RS01795 0.81 -0.2 403,764 + +0.5 403,764 + +0.5 403,764 + +1.0 403,764 + +0.2 403,764 + -0.3 403,764 + -0.9 403,764 + +0.4 403,764 + +1.5 403,765 - -0.3 403,765 - -0.2 403,765 - -0.9 403,765 - +0.7 403,765 - -1.1 403,765 - +1.0 403,765 - -0.7 403,765 - +0.8 403,765 - +0.6 403,765 - -1.5 403,765 - +1.8 403,765 - -0.6 403,765 - -0.8 403,765 - +0.4 403,765 - +0.3 403,765 - -0.0 403,872 - -0.0 403,942 + +0.5 403,942 + +1.0 403,942 + -0.6 403,943 - +1.5 403,943 - -0.2 403,943 - +0.7 403,943 - +0.4
Or see this region's nucleotide sequence