Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3079 and Dsui_3080 are separated by 145 nucleotides Dsui_3080 and Dsui_3081 are separated by 141 nucleotides
Dsui_3079: Dsui_3079 - serine/threonine protein kinase, at 3,295,256 to 3,296,653
_3079
Dsui_3080: Dsui_3080 - molecular chaperone GrpE (heat shock protein), at 3,296,799 to 3,297,356
_3080
Dsui_3081: Dsui_3081 - chaperone protein DnaK, at 3,297,498 to 3,299,429
_3081
Position (kb)
3296
3297
3298 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3295.818 kb on + strand, within Dsui_3079 at 3295.826 kb on - strand, within Dsui_3079 at 3295.845 kb on - strand, within Dsui_3079 at 3295.934 kb on + strand, within Dsui_3079 at 3295.942 kb on - strand, within Dsui_3079 at 3295.952 kb on + strand, within Dsui_3079 at 3295.975 kb on - strand, within Dsui_3079 at 3296.040 kb on - strand, within Dsui_3079 at 3296.073 kb on + strand at 3296.073 kb on + strand, within Dsui_3079 at 3296.073 kb on + strand, within Dsui_3079 at 3296.073 kb on + strand at 3296.073 kb on + strand at 3296.073 kb on + strand at 3296.073 kb on + strand, within Dsui_3079 at 3296.073 kb on + strand, within Dsui_3079 at 3296.075 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.081 kb on - strand, within Dsui_3079 at 3296.145 kb on + strand, within Dsui_3079 at 3296.147 kb on + strand at 3296.147 kb on - strand, within Dsui_3079 at 3296.188 kb on - strand, within Dsui_3079 at 3296.261 kb on + strand, within Dsui_3079 at 3296.261 kb on + strand, within Dsui_3079 at 3296.274 kb on - strand, within Dsui_3079 at 3296.315 kb on - strand, within Dsui_3079 at 3296.328 kb on + strand, within Dsui_3079 at 3296.328 kb on + strand at 3296.333 kb on + strand at 3296.336 kb on - strand, within Dsui_3079 at 3296.341 kb on - strand, within Dsui_3079 at 3296.371 kb on - strand, within Dsui_3079 at 3296.379 kb on - strand, within Dsui_3079 at 3296.384 kb on + strand, within Dsui_3079 at 3296.386 kb on + strand, within Dsui_3079 at 3296.547 kb on - strand at 3296.565 kb on - strand at 3296.567 kb on - strand at 3296.625 kb on + strand at 3296.662 kb on - strand at 3296.692 kb on + strand at 3296.704 kb on + strand at 3296.713 kb on - strand at 3296.921 kb on + strand, within Dsui_3080 at 3297.341 kb on + strand at 3297.341 kb on + strand at 3297.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 3,295,818 + Dsui_3079 0.40 -3.8 3,295,826 - Dsui_3079 0.41 +0.6 3,295,845 - Dsui_3079 0.42 +0.4 3,295,934 + Dsui_3079 0.48 -2.0 3,295,942 - Dsui_3079 0.49 +0.9 3,295,952 + Dsui_3079 0.50 -2.6 3,295,975 - Dsui_3079 0.51 +0.3 3,296,040 - Dsui_3079 0.56 +0.6 3,296,073 + -2.7 3,296,073 + Dsui_3079 0.58 -1.0 3,296,073 + Dsui_3079 0.58 -0.0 3,296,073 + -1.9 3,296,073 + -1.6 3,296,073 + -2.2 3,296,073 + Dsui_3079 0.58 -2.3 3,296,073 + Dsui_3079 0.58 -1.7 3,296,075 - Dsui_3079 0.59 +0.6 3,296,081 - Dsui_3079 0.59 -0.5 3,296,081 - Dsui_3079 0.59 +1.1 3,296,081 - Dsui_3079 0.59 +1.6 3,296,081 - Dsui_3079 0.59 +0.6 3,296,081 - Dsui_3079 0.59 +1.5 3,296,081 - Dsui_3079 0.59 +0.8 3,296,081 - Dsui_3079 0.59 +1.1 3,296,145 + Dsui_3079 0.64 -2.0 3,296,147 + +3.0 3,296,147 - Dsui_3079 0.64 +0.5 3,296,188 - Dsui_3079 0.67 +0.5 3,296,261 + Dsui_3079 0.72 -0.2 3,296,261 + Dsui_3079 0.72 -3.2 3,296,274 - Dsui_3079 0.73 +1.0 3,296,315 - Dsui_3079 0.76 +0.5 3,296,328 + Dsui_3079 0.77 -2.8 3,296,328 + -0.6 3,296,333 + -2.8 3,296,336 - Dsui_3079 0.77 -0.4 3,296,341 - Dsui_3079 0.78 +0.9 3,296,371 - Dsui_3079 0.80 +0.0 3,296,379 - Dsui_3079 0.80 +0.4 3,296,384 + Dsui_3079 0.81 -1.4 3,296,386 + Dsui_3079 0.81 +0.7 3,296,547 - +0.1 3,296,565 - +0.1 3,296,567 - +1.0 3,296,625 + -2.8 3,296,662 - +1.2 3,296,692 + +0.4 3,296,704 + -2.8 3,296,713 - -1.1 3,296,921 + Dsui_3080 0.22 -3.7 3,297,341 + +0.2 3,297,341 + +0.5 3,297,479 + -0.3
Or see this region's nucleotide sequence