Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3065 and Dsui_3066 are separated by 247 nucleotides Dsui_3066 and Dsui_3067 are separated by 3 nucleotides Dsui_3067 and Dsui_3068 are separated by 94 nucleotides
Dsui_3065: Dsui_3065 - carbamoyl-phosphate synthase, small subunit, at 3,281,423 to 3,282,571
_3065
Dsui_3066: Dsui_3066 - dihydrodipicolinate reductase, at 3,282,819 to 3,283,625
_3066
Dsui_3067: Dsui_3067 - small protein A (tmRNA-binding), at 3,283,629 to 3,284,159
_3067
Dsui_3068: Dsui_3068 - Fe2+/Zn2+ uptake regulation protein, at 3,284,254 to 3,284,724
_3068
Position (kb)
3282
3283
3284 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3282.740 kb on - strand at 3282.783 kb on + strand at 3282.783 kb on + strand at 3283.056 kb on + strand, within Dsui_3066 at 3283.056 kb on + strand, within Dsui_3066 at 3283.099 kb on - strand, within Dsui_3066 at 3283.119 kb on - strand, within Dsui_3066 at 3283.252 kb on - strand, within Dsui_3066 at 3283.421 kb on + strand at 3283.429 kb on - strand, within Dsui_3066 at 3283.429 kb on - strand, within Dsui_3066 at 3283.459 kb on + strand at 3283.467 kb on - strand, within Dsui_3066 at 3283.467 kb on - strand, within Dsui_3066 at 3283.467 kb on - strand, within Dsui_3066 at 3283.467 kb on - strand, within Dsui_3066 at 3283.550 kb on + strand at 3283.550 kb on + strand at 3283.580 kb on - strand at 3283.647 kb on - strand at 3283.697 kb on - strand, within Dsui_3067 at 3283.725 kb on - strand, within Dsui_3067 at 3284.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 3,282,740 - +2.3 3,282,783 + +0.1 3,282,783 + -1.6 3,283,056 + Dsui_3066 0.29 -3.4 3,283,056 + Dsui_3066 0.29 -2.4 3,283,099 - Dsui_3066 0.35 -3.3 3,283,119 - Dsui_3066 0.37 -4.3 3,283,252 - Dsui_3066 0.54 -3.0 3,283,421 + -1.9 3,283,429 - Dsui_3066 0.76 -1.8 3,283,429 - Dsui_3066 0.76 -2.0 3,283,459 + -3.0 3,283,467 - Dsui_3066 0.80 -3.1 3,283,467 - Dsui_3066 0.80 +1.0 3,283,467 - Dsui_3066 0.80 -2.1 3,283,467 - Dsui_3066 0.80 -2.4 3,283,550 + -5.8 3,283,550 + -2.6 3,283,580 - -1.2 3,283,647 - +0.7 3,283,697 - Dsui_3067 0.13 +1.0 3,283,725 - Dsui_3067 0.18 -0.3 3,284,245 + +0.3
Or see this region's nucleotide sequence