Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2963 and Dsui_2964 are separated by 3 nucleotides Dsui_2964 and Dsui_2965 are separated by 28 nucleotides Dsui_2965 and Dsui_2966 overlap by 4 nucleotides Dsui_2966 and Dsui_2967 are separated by 41 nucleotides
Dsui_2963: Dsui_2963 - precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit,precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit, at 3,170,916 to 3,172,208
_2963
Dsui_2964: Dsui_2964 - precorrin-2 C20-methyltransferase, at 3,172,212 to 3,173,012
_2964
Dsui_2965: Dsui_2965 - cobalamin biosynthesis protein CbiG, at 3,173,041 to 3,173,826
_2965
Dsui_2966: Dsui_2966 - cobalamin biosynthesis protein CbiG, at 3,173,823 to 3,174,230
_2966
Dsui_2967: Dsui_2967 - precorrin-3B C17-methyltransferase, at 3,174,272 to 3,175,267
_2967
Position (kb)
3173
3174 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3172.068 kb on + strand, within Dsui_2963 at 3172.129 kb on + strand at 3172.210 kb on - strand at 3172.359 kb on - strand, within Dsui_2964 at 3172.381 kb on + strand, within Dsui_2964 at 3172.498 kb on + strand, within Dsui_2964 at 3172.603 kb on - strand at 3172.668 kb on + strand, within Dsui_2964 at 3172.808 kb on + strand, within Dsui_2964 at 3172.914 kb on - strand, within Dsui_2964 at 3173.016 kb on + strand at 3173.501 kb on + strand, within Dsui_2965 at 3173.693 kb on - strand at 3173.707 kb on + strand, within Dsui_2965 at 3173.710 kb on - strand, within Dsui_2965 at 3173.829 kb on - strand at 3173.940 kb on - strand at 3173.965 kb on + strand, within Dsui_2966 at 3173.965 kb on + strand, within Dsui_2966 at 3174.018 kb on + strand, within Dsui_2966 at 3174.021 kb on - strand at 3174.057 kb on + strand, within Dsui_2966 at 3174.299 kb on + strand at 3174.306 kb on - strand at 3174.476 kb on + strand, within Dsui_2967 at 3174.505 kb on - strand, within Dsui_2967 at 3174.505 kb on - strand, within Dsui_2967 at 3174.694 kb on - strand at 3174.777 kb on - strand at 3174.791 kb on + strand, within Dsui_2967 at 3174.796 kb on + strand, within Dsui_2967 at 3174.802 kb on + strand, within Dsui_2967 at 3174.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 3,172,068 + Dsui_2963 0.89 -0.4 3,172,129 + +1.0 3,172,210 - -2.6 3,172,359 - Dsui_2964 0.18 -2.4 3,172,381 + Dsui_2964 0.21 -2.2 3,172,498 + Dsui_2964 0.36 -0.8 3,172,603 - -0.9 3,172,668 + Dsui_2964 0.57 -0.4 3,172,808 + Dsui_2964 0.74 -1.7 3,172,914 - Dsui_2964 0.88 +0.0 3,173,016 + +0.3 3,173,501 + Dsui_2965 0.59 -1.5 3,173,693 - -2.5 3,173,707 + Dsui_2965 0.85 -0.2 3,173,710 - Dsui_2965 0.85 -2.3 3,173,829 - -2.2 3,173,940 - -3.4 3,173,965 + Dsui_2966 0.35 -0.3 3,173,965 + Dsui_2966 0.35 +0.5 3,174,018 + Dsui_2966 0.48 +1.2 3,174,021 - -2.8 3,174,057 + Dsui_2966 0.57 +1.9 3,174,299 + -0.3 3,174,306 - -1.2 3,174,476 + Dsui_2967 0.20 -1.5 3,174,505 - Dsui_2967 0.23 -0.5 3,174,505 - Dsui_2967 0.23 -1.1 3,174,694 - -3.1 3,174,777 - -2.1 3,174,791 + Dsui_2967 0.52 -0.4 3,174,796 + Dsui_2967 0.53 -1.0 3,174,802 + Dsui_2967 0.53 -0.0 3,174,805 - -2.8
Or see this region's nucleotide sequence