Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2590 and Dsui_2591 are separated by 612 nucleotides Dsui_2591 and Dsui_2592 overlap by 1 nucleotides
Dsui_2590: Dsui_2590 - Pilin accessory protein (PilO), at 2,743,997 to 2,745,409
_2590
Dsui_2591: Dsui_2591 - type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,746,022 to 2,747,608
_2591
Dsui_2592: Dsui_2592 - type II secretory pathway, component PulF, at 2,747,608 to 2,748,720
_2592
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2745.216 kb on - strand, within Dsui_2590 at 2745.221 kb on - strand, within Dsui_2590 at 2745.274 kb on + strand at 2745.394 kb on + strand at 2745.572 kb on - strand at 2745.601 kb on - strand at 2745.787 kb on + strand at 2745.846 kb on + strand at 2745.872 kb on + strand at 2745.914 kb on + strand at 2746.008 kb on + strand at 2746.088 kb on + strand at 2746.142 kb on - strand at 2746.148 kb on + strand at 2746.217 kb on + strand, within Dsui_2591 at 2746.429 kb on + strand at 2746.611 kb on - strand, within Dsui_2591 at 2746.761 kb on + strand, within Dsui_2591 at 2746.788 kb on - strand, within Dsui_2591 at 2746.802 kb on + strand at 2746.805 kb on - strand, within Dsui_2591 at 2746.887 kb on - strand, within Dsui_2591 at 2746.889 kb on + strand, within Dsui_2591 at 2746.907 kb on + strand, within Dsui_2591 at 2746.928 kb on - strand, within Dsui_2591 at 2746.974 kb on - strand, within Dsui_2591 at 2747.118 kb on + strand, within Dsui_2591 at 2747.157 kb on + strand, within Dsui_2591 at 2747.405 kb on - strand, within Dsui_2591 at 2747.534 kb on - strand at 2747.713 kb on + strand at 2747.721 kb on - strand, within Dsui_2592 at 2747.746 kb on - strand, within Dsui_2592 at 2747.771 kb on - strand, within Dsui_2592 at 2747.801 kb on - strand, within Dsui_2592 at 2747.989 kb on + strand, within Dsui_2592 at 2748.044 kb on - strand, within Dsui_2592 at 2748.044 kb on - strand, within Dsui_2592 at 2748.046 kb on + strand, within Dsui_2592 at 2748.065 kb on - strand, within Dsui_2592 at 2748.084 kb on + strand, within Dsui_2592 at 2748.261 kb on - strand, within Dsui_2592 at 2748.314 kb on - strand, within Dsui_2592 at 2748.314 kb on - strand, within Dsui_2592 at 2748.324 kb on - strand, within Dsui_2592 at 2748.334 kb on + strand, within Dsui_2592 at 2748.357 kb on + strand, within Dsui_2592 at 2748.373 kb on - strand, within Dsui_2592 at 2748.487 kb on - strand, within Dsui_2592
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,745,216 - Dsui_2590 0.86 -3.3 2,745,221 - Dsui_2590 0.87 -2.7 2,745,274 + -1.5 2,745,394 + +1.3 2,745,572 - +3.1 2,745,601 - -1.1 2,745,787 + -0.3 2,745,846 + -0.9 2,745,872 + +0.6 2,745,914 + -2.3 2,746,008 + -1.8 2,746,088 + +3.1 2,746,142 - -0.3 2,746,148 + -1.0 2,746,217 + Dsui_2591 0.12 -0.6 2,746,429 + -2.0 2,746,611 - Dsui_2591 0.37 -3.4 2,746,761 + Dsui_2591 0.47 -1.3 2,746,788 - Dsui_2591 0.48 -1.0 2,746,802 + +1.6 2,746,805 - Dsui_2591 0.49 +0.3 2,746,887 - Dsui_2591 0.55 -3.4 2,746,889 + Dsui_2591 0.55 -0.5 2,746,907 + Dsui_2591 0.56 +2.7 2,746,928 - Dsui_2591 0.57 -3.6 2,746,974 - Dsui_2591 0.60 -1.0 2,747,118 + Dsui_2591 0.69 -1.0 2,747,157 + Dsui_2591 0.72 -3.0 2,747,405 - Dsui_2591 0.87 -0.7 2,747,534 - -0.3 2,747,713 + +3.0 2,747,721 - Dsui_2592 0.10 -0.1 2,747,746 - Dsui_2592 0.12 -3.2 2,747,771 - Dsui_2592 0.15 -0.4 2,747,801 - Dsui_2592 0.17 -2.1 2,747,989 + Dsui_2592 0.34 -0.7 2,748,044 - Dsui_2592 0.39 +1.1 2,748,044 - Dsui_2592 0.39 -1.0 2,748,046 + Dsui_2592 0.39 -3.0 2,748,065 - Dsui_2592 0.41 -2.8 2,748,084 + Dsui_2592 0.43 +1.6 2,748,261 - Dsui_2592 0.59 -0.8 2,748,314 - Dsui_2592 0.63 -0.2 2,748,314 - Dsui_2592 0.63 -0.9 2,748,324 - Dsui_2592 0.64 -0.1 2,748,334 + Dsui_2592 0.65 -2.5 2,748,357 + Dsui_2592 0.67 -1.3 2,748,373 - Dsui_2592 0.69 -0.0 2,748,487 - Dsui_2592 0.79 -1.7
Or see this region's nucleotide sequence