Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2501 and Dsui_2502 overlap by 4 nucleotides Dsui_2502 and Dsui_2503 are separated by 2 nucleotides Dsui_2503 and Dsui_2504 are separated by 244 nucleotides Dsui_2504 and Dsui_2505 overlap by 17 nucleotides
Dsui_2501: Dsui_2501 - TniQ protein, at 2,648,166 to 2,649,332
_2501
Dsui_2502: Dsui_2502 - TniB protein, at 2,649,329 to 2,650,237
_2502
Dsui_2503: Dsui_2503 - Mu transposase/integrase, at 2,650,240 to 2,651,922
_2503
Dsui_2504: Dsui_2504 - hypothetical protein, at 2,652,167 to 2,652,547
_2504
Dsui_2505: Dsui_2505 - putative transcriptional regulator, at 2,652,531 to 2,652,830
_2505
Position (kb)
2650
2651
2652 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2649.364 kb on + strand at 2649.566 kb on + strand, within Dsui_2502 at 2649.703 kb on + strand, within Dsui_2502 at 2649.711 kb on - strand, within Dsui_2502 at 2649.824 kb on - strand, within Dsui_2502 at 2650.079 kb on + strand, within Dsui_2502 at 2650.216 kb on - strand at 2650.238 kb on + strand at 2650.681 kb on + strand at 2650.898 kb on + strand, within Dsui_2503 at 2651.250 kb on + strand at 2651.457 kb on - strand, within Dsui_2503 at 2651.474 kb on + strand, within Dsui_2503 at 2651.492 kb on - strand, within Dsui_2503 at 2651.717 kb on - strand, within Dsui_2503 at 2651.761 kb on - strand at 2651.771 kb on - strand at 2651.771 kb on - strand at 2651.816 kb on - strand at 2651.945 kb on + strand at 2651.945 kb on + strand at 2651.965 kb on - strand at 2652.015 kb on + strand at 2652.015 kb on - strand at 2652.041 kb on + strand at 2652.206 kb on + strand, within Dsui_2504 at 2652.278 kb on + strand, within Dsui_2504 at 2652.314 kb on - strand at 2652.417 kb on + strand, within Dsui_2504 at 2652.443 kb on + strand, within Dsui_2504 at 2652.462 kb on + strand, within Dsui_2504 at 2652.470 kb on - strand, within Dsui_2504 at 2652.495 kb on - strand at 2652.495 kb on - strand, within Dsui_2504 at 2652.495 kb on - strand, within Dsui_2504
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,649,364 + -0.6 2,649,566 + Dsui_2502 0.26 -0.3 2,649,703 + Dsui_2502 0.41 +0.4 2,649,711 - Dsui_2502 0.42 +0.5 2,649,824 - Dsui_2502 0.54 -3.2 2,650,079 + Dsui_2502 0.83 -3.3 2,650,216 - -0.4 2,650,238 + +0.1 2,650,681 + -1.2 2,650,898 + Dsui_2503 0.39 -2.7 2,651,250 + +1.4 2,651,457 - Dsui_2503 0.72 -0.4 2,651,474 + Dsui_2503 0.73 -1.1 2,651,492 - Dsui_2503 0.74 -1.7 2,651,717 - Dsui_2503 0.88 -0.8 2,651,761 - +1.4 2,651,771 - -2.7 2,651,771 - -3.0 2,651,816 - -0.2 2,651,945 + -1.4 2,651,945 + -1.7 2,651,965 - -1.4 2,652,015 + -3.2 2,652,015 - -0.2 2,652,041 + -1.7 2,652,206 + Dsui_2504 0.10 +1.1 2,652,278 + Dsui_2504 0.29 +1.0 2,652,314 - -1.6 2,652,417 + Dsui_2504 0.66 +0.9 2,652,443 + Dsui_2504 0.72 +1.0 2,652,462 + Dsui_2504 0.77 -1.6 2,652,470 - Dsui_2504 0.80 -0.7 2,652,495 - -1.4 2,652,495 - Dsui_2504 0.86 -3.4 2,652,495 - Dsui_2504 0.86 -2.3
Or see this region's nucleotide sequence