Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2441 and Dsui_2442 are separated by 12 nucleotides Dsui_2442 and Dsui_2443 are separated by 165 nucleotides Dsui_2443 and Dsui_2444 are separated by 20 nucleotides Dsui_2444 and Dsui_2445 are separated by 77 nucleotides
Dsui_2441: Dsui_2441 - putative methyltransferase, at 2,602,151 to 2,602,879
_2441
Dsui_2442: Dsui_2442 - MAF protein, at 2,602,892 to 2,603,485
_2442
Dsui_2443: Dsui_2443 - putative metal-binding protein, possibly nucleic-acid binding protein, at 2,603,651 to 2,604,142
_2443
Dsui_2444: Dsui_2444 - ribosomal protein L32, at 2,604,163 to 2,604,342
_2444
Dsui_2445: Dsui_2445 - fatty acid/phospholipid synthesis protein PlsX, at 2,604,420 to 2,605,460
_2445
Position (kb)
2603
2604
2605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2602.742 kb on - strand, within Dsui_2441 at 2602.749 kb on + strand, within Dsui_2441 at 2602.767 kb on + strand, within Dsui_2441 at 2602.775 kb on - strand, within Dsui_2441 at 2602.775 kb on - strand, within Dsui_2441 at 2602.775 kb on - strand, within Dsui_2441 at 2602.788 kb on + strand, within Dsui_2441 at 2602.796 kb on - strand, within Dsui_2441 at 2602.804 kb on + strand, within Dsui_2441 at 2602.870 kb on - strand at 2602.915 kb on - strand at 2602.929 kb on - strand at 2602.946 kb on - strand at 2602.946 kb on - strand at 2603.033 kb on + strand, within Dsui_2442 at 2603.033 kb on + strand, within Dsui_2442 at 2603.033 kb on + strand, within Dsui_2442 at 2603.033 kb on + strand, within Dsui_2442 at 2603.041 kb on - strand, within Dsui_2442 at 2603.109 kb on - strand, within Dsui_2442 at 2603.109 kb on - strand, within Dsui_2442 at 2603.112 kb on + strand, within Dsui_2442 at 2603.133 kb on + strand, within Dsui_2442 at 2603.197 kb on - strand, within Dsui_2442 at 2603.211 kb on + strand at 2603.212 kb on + strand, within Dsui_2442 at 2603.220 kb on - strand, within Dsui_2442 at 2603.245 kb on + strand, within Dsui_2442 at 2603.253 kb on - strand, within Dsui_2442 at 2603.258 kb on - strand, within Dsui_2442 at 2603.291 kb on + strand, within Dsui_2442 at 2603.309 kb on - strand, within Dsui_2442 at 2603.312 kb on + strand, within Dsui_2442 at 2603.315 kb on - strand, within Dsui_2442 at 2603.315 kb on - strand, within Dsui_2442 at 2603.315 kb on - strand, within Dsui_2442 at 2603.320 kb on - strand, within Dsui_2442 at 2603.329 kb on + strand, within Dsui_2442 at 2603.339 kb on + strand, within Dsui_2442 at 2603.352 kb on - strand, within Dsui_2442 at 2603.410 kb on - strand, within Dsui_2442 at 2603.410 kb on - strand, within Dsui_2442 at 2603.411 kb on - strand, within Dsui_2442 at 2603.417 kb on + strand at 2603.425 kb on - strand, within Dsui_2442 at 2603.459 kb on - strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.466 kb on + strand at 2603.474 kb on - strand at 2603.474 kb on - strand at 2603.474 kb on - strand at 2603.474 kb on - strand at 2603.474 kb on - strand at 2603.474 kb on - strand at 2603.512 kb on + strand at 2603.520 kb on - strand at 2603.520 kb on - strand at 2603.530 kb on - strand at 2603.534 kb on + strand at 2603.566 kb on + strand at 2603.566 kb on + strand at 2603.662 kb on + strand at 2603.675 kb on + strand at 2603.752 kb on + strand, within Dsui_2443 at 2603.771 kb on + strand, within Dsui_2443
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,602,742 - Dsui_2441 0.81 +0.1 2,602,749 + Dsui_2441 0.82 +0.7 2,602,767 + Dsui_2441 0.84 -0.5 2,602,775 - Dsui_2441 0.86 +0.5 2,602,775 - Dsui_2441 0.86 +0.1 2,602,775 - Dsui_2441 0.86 +0.7 2,602,788 + Dsui_2441 0.87 -1.2 2,602,796 - Dsui_2441 0.88 +0.5 2,602,804 + Dsui_2441 0.90 +0.1 2,602,870 - +1.0 2,602,915 - -2.6 2,602,929 - +0.8 2,602,946 - -0.7 2,602,946 - +0.3 2,603,033 + Dsui_2442 0.24 -0.1 2,603,033 + Dsui_2442 0.24 +1.1 2,603,033 + Dsui_2442 0.24 +2.0 2,603,033 + Dsui_2442 0.24 -1.2 2,603,041 - Dsui_2442 0.25 +0.8 2,603,109 - Dsui_2442 0.37 +0.3 2,603,109 - Dsui_2442 0.37 +0.4 2,603,112 + Dsui_2442 0.37 -2.1 2,603,133 + Dsui_2442 0.41 +0.2 2,603,197 - Dsui_2442 0.51 -1.5 2,603,211 + -1.1 2,603,212 + Dsui_2442 0.54 -3.3 2,603,220 - Dsui_2442 0.55 +0.9 2,603,245 + Dsui_2442 0.59 -1.2 2,603,253 - Dsui_2442 0.61 +0.9 2,603,258 - Dsui_2442 0.62 -0.4 2,603,291 + Dsui_2442 0.67 -2.8 2,603,309 - Dsui_2442 0.70 +0.5 2,603,312 + Dsui_2442 0.71 -1.9 2,603,315 - Dsui_2442 0.71 +0.2 2,603,315 - Dsui_2442 0.71 +0.2 2,603,315 - Dsui_2442 0.71 -0.8 2,603,320 - Dsui_2442 0.72 -0.3 2,603,329 + Dsui_2442 0.74 -2.4 2,603,339 + Dsui_2442 0.75 +0.9 2,603,352 - Dsui_2442 0.77 +0.6 2,603,410 - Dsui_2442 0.87 +0.3 2,603,410 - Dsui_2442 0.87 +1.1 2,603,411 - Dsui_2442 0.87 -0.2 2,603,417 + -0.1 2,603,425 - Dsui_2442 0.90 -1.5 2,603,459 - -1.1 2,603,466 + -1.5 2,603,466 + -1.0 2,603,466 + +0.7 2,603,466 + +1.6 2,603,466 + -3.1 2,603,466 + +1.5 2,603,466 + -2.9 2,603,474 - +1.2 2,603,474 - -0.6 2,603,474 - +1.5 2,603,474 - +1.3 2,603,474 - -0.9 2,603,474 - -0.7 2,603,512 + -1.2 2,603,520 - -0.6 2,603,520 - +1.2 2,603,530 - +0.4 2,603,534 + -4.0 2,603,566 + -1.2 2,603,566 + -0.3 2,603,662 + -0.9 2,603,675 + -1.4 2,603,752 + Dsui_2443 0.21 -3.5 2,603,771 + Dsui_2443 0.24 -2.2
Or see this region's nucleotide sequence