Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2410 and Dsui_2411 are separated by 121 nucleotides Dsui_2411 and Dsui_2412 are separated by 125 nucleotides
Dsui_2410: Dsui_2410 - transcriptional regulator, at 2,561,325 to 2,562,227
_2410
Dsui_2411: Dsui_2411 - alanine racemase, at 2,562,349 to 2,563,440
_2411
Dsui_2412: Dsui_2412 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 2,563,566 to 2,566,304
_2412
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2561.388 kb on + strand at 2561.396 kb on + strand at 2561.401 kb on + strand at 2561.422 kb on + strand, within Dsui_2410 at 2561.519 kb on + strand, within Dsui_2410 at 2561.596 kb on + strand, within Dsui_2410 at 2561.649 kb on + strand, within Dsui_2410 at 2561.743 kb on + strand, within Dsui_2410 at 2561.743 kb on + strand, within Dsui_2410 at 2561.743 kb on + strand, within Dsui_2410 at 2561.748 kb on + strand, within Dsui_2410 at 2561.874 kb on + strand, within Dsui_2410 at 2561.923 kb on + strand, within Dsui_2410 at 2561.923 kb on + strand, within Dsui_2410 at 2561.923 kb on + strand, within Dsui_2410 at 2561.954 kb on + strand, within Dsui_2410 at 2562.011 kb on + strand, within Dsui_2410 at 2562.034 kb on + strand, within Dsui_2410 at 2562.054 kb on + strand, within Dsui_2410 at 2562.075 kb on + strand, within Dsui_2410 at 2562.107 kb on + strand, within Dsui_2410 at 2562.128 kb on + strand, within Dsui_2410 at 2562.220 kb on - strand at 2563.445 kb on + strand at 2563.622 kb on - strand at 2563.694 kb on + strand at 2563.923 kb on + strand, within Dsui_2412 at 2564.225 kb on + strand, within Dsui_2412 at 2564.281 kb on - strand, within Dsui_2412 at 2564.412 kb on + strand, within Dsui_2412 at 2564.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,561,388 + -1.2 2,561,396 + -1.2 2,561,401 + -2.5 2,561,422 + Dsui_2410 0.11 -0.7 2,561,519 + Dsui_2410 0.21 -1.2 2,561,596 + Dsui_2410 0.30 +0.7 2,561,649 + Dsui_2410 0.36 -1.5 2,561,743 + Dsui_2410 0.46 -0.7 2,561,743 + Dsui_2410 0.46 -2.6 2,561,743 + Dsui_2410 0.46 -1.6 2,561,748 + Dsui_2410 0.47 -2.9 2,561,874 + Dsui_2410 0.61 -0.3 2,561,923 + Dsui_2410 0.66 -1.2 2,561,923 + Dsui_2410 0.66 +0.3 2,561,923 + Dsui_2410 0.66 -0.1 2,561,954 + Dsui_2410 0.70 -0.5 2,562,011 + Dsui_2410 0.76 +0.5 2,562,034 + Dsui_2410 0.79 -1.4 2,562,054 + Dsui_2410 0.81 -1.2 2,562,075 + Dsui_2410 0.83 +0.2 2,562,107 + Dsui_2410 0.87 +0.5 2,562,128 + Dsui_2410 0.89 -1.5 2,562,220 - -0.5 2,563,445 + +0.4 2,563,622 - -0.5 2,563,694 + -4.1 2,563,923 + Dsui_2412 0.13 +0.1 2,564,225 + Dsui_2412 0.24 +0.4 2,564,281 - Dsui_2412 0.26 -3.5 2,564,412 + Dsui_2412 0.31 -0.0 2,564,415 - -1.4
Or see this region's nucleotide sequence