Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2350 and Dsui_2351 overlap by 50 nucleotides Dsui_2351 and Dsui_2352 are separated by 121 nucleotides Dsui_2352 and Dsui_2353 are separated by 6 nucleotides
Dsui_2350: Dsui_2350 - putative transcriptional regulator, at 2,503,453 to 2,503,917
_2350
Dsui_2351: Dsui_2351 - uncharacterized protein, gamma-carboxymuconolactone decarboxylase subunit like protein, at 2,503,868 to 2,504,242
_2351
Dsui_2352: Dsui_2352 - hypothetical protein, at 2,504,364 to 2,505,068
_2352
Dsui_2353: Dsui_2353 - transcriptional regulator, at 2,505,075 to 2,505,974
_2353
Position (kb)
2504
2505
2506 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2503.515 kb on + strand, within Dsui_2350 at 2503.539 kb on + strand at 2503.681 kb on + strand, within Dsui_2350 at 2503.725 kb on + strand at 2503.812 kb on + strand at 2503.951 kb on + strand at 2504.091 kb on + strand at 2504.091 kb on + strand at 2504.126 kb on + strand at 2504.136 kb on + strand at 2504.379 kb on + strand at 2504.615 kb on - strand at 2504.677 kb on + strand at 2504.853 kb on + strand, within Dsui_2352 at 2504.867 kb on + strand, within Dsui_2352 at 2504.869 kb on - strand, within Dsui_2352 at 2504.889 kb on + strand, within Dsui_2352 at 2504.889 kb on + strand at 2504.906 kb on + strand at 2504.961 kb on + strand, within Dsui_2352 at 2504.984 kb on + strand at 2504.996 kb on + strand, within Dsui_2352 at 2505.012 kb on + strand at 2505.335 kb on + strand at 2505.368 kb on + strand, within Dsui_2353 at 2505.368 kb on + strand, within Dsui_2353 at 2505.368 kb on + strand, within Dsui_2353 at 2505.376 kb on - strand, within Dsui_2353 at 2505.404 kb on - strand, within Dsui_2353 at 2505.435 kb on + strand at 2505.468 kb on + strand at 2505.470 kb on - strand, within Dsui_2353 at 2505.483 kb on - strand, within Dsui_2353 at 2505.506 kb on - strand, within Dsui_2353 at 2505.565 kb on + strand, within Dsui_2353 at 2505.610 kb on - strand, within Dsui_2353 at 2505.610 kb on - strand, within Dsui_2353 at 2505.688 kb on + strand, within Dsui_2353 at 2505.696 kb on - strand, within Dsui_2353 at 2505.696 kb on - strand, within Dsui_2353 at 2505.704 kb on + strand at 2505.715 kb on + strand, within Dsui_2353 at 2505.730 kb on - strand, within Dsui_2353 at 2505.884 kb on + strand, within Dsui_2353 at 2505.892 kb on - strand at 2505.930 kb on + strand at 2505.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,503,515 + Dsui_2350 0.13 -0.9 2,503,539 + +0.4 2,503,681 + Dsui_2350 0.49 -0.8 2,503,725 + +1.4 2,503,812 + -1.9 2,503,951 + -0.9 2,504,091 + -2.4 2,504,091 + -0.9 2,504,126 + -1.1 2,504,136 + -0.5 2,504,379 + -1.7 2,504,615 - -2.7 2,504,677 + -2.1 2,504,853 + Dsui_2352 0.69 -0.9 2,504,867 + Dsui_2352 0.71 -1.6 2,504,869 - Dsui_2352 0.72 -0.9 2,504,889 + Dsui_2352 0.74 -2.9 2,504,889 + -1.7 2,504,906 + -2.3 2,504,961 + Dsui_2352 0.85 -1.6 2,504,984 + -0.1 2,504,996 + Dsui_2352 0.90 -1.6 2,505,012 + -0.4 2,505,335 + -1.9 2,505,368 + Dsui_2353 0.33 +0.5 2,505,368 + Dsui_2353 0.33 +0.7 2,505,368 + Dsui_2353 0.33 -2.1 2,505,376 - Dsui_2353 0.33 -1.3 2,505,404 - Dsui_2353 0.37 +1.6 2,505,435 + -2.7 2,505,468 + -0.5 2,505,470 - Dsui_2353 0.44 +0.5 2,505,483 - Dsui_2353 0.45 +0.0 2,505,506 - Dsui_2353 0.48 +0.4 2,505,565 + Dsui_2353 0.54 -0.6 2,505,610 - Dsui_2353 0.59 +1.5 2,505,610 - Dsui_2353 0.59 +1.3 2,505,688 + Dsui_2353 0.68 -3.8 2,505,696 - Dsui_2353 0.69 +0.1 2,505,696 - Dsui_2353 0.69 +0.8 2,505,704 + -1.7 2,505,715 + Dsui_2353 0.71 -2.8 2,505,730 - Dsui_2353 0.73 -0.4 2,505,884 + Dsui_2353 0.90 -1.4 2,505,892 - +1.1 2,505,930 + -0.9 2,505,994 - -0.1
Or see this region's nucleotide sequence