Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2168 and Dsui_2169 are separated by 71 nucleotides Dsui_2169 and Dsui_2170 overlap by 4 nucleotides Dsui_2170 and Dsui_2171 overlap by 4 nucleotides Dsui_2171 and Dsui_2172 are separated by 37 nucleotides
Dsui_2168: Dsui_2168 - putative hydrolase of alpha/beta superfamily, at 2,305,254 to 2,306,219
_2168
Dsui_2169: Dsui_2169 - hypothetical protein, at 2,306,291 to 2,306,572
_2169
Dsui_2170: Dsui_2170 - putative iron-regulated membrane protein, at 2,306,569 to 2,308,077
_2170
Dsui_2171: Dsui_2171 - Protein of unknown function (DUF3325), at 2,308,074 to 2,308,430
_2171
Dsui_2172: Dsui_2172 - transcriptional regulator, at 2,308,468 to 2,308,944
_2172
Position (kb)
2306
2307
2308
2309 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2305.596 kb on - strand at 2305.687 kb on - strand at 2306.214 kb on + strand at 2306.305 kb on + strand at 2306.407 kb on - strand at 2306.500 kb on + strand at 2306.838 kb on - strand at 2306.883 kb on - strand at 2306.938 kb on + strand, within Dsui_2170 at 2306.963 kb on + strand at 2307.021 kb on + strand at 2307.091 kb on - strand at 2307.109 kb on - strand, within Dsui_2170 at 2307.109 kb on - strand at 2307.470 kb on + strand at 2307.470 kb on + strand at 2307.470 kb on + strand at 2307.532 kb on + strand at 2307.594 kb on + strand at 2307.891 kb on + strand, within Dsui_2170 at 2308.009 kb on + strand at 2308.017 kb on - strand at 2308.113 kb on - strand at 2308.129 kb on + strand, within Dsui_2171 at 2308.135 kb on + strand at 2308.392 kb on + strand at 2308.409 kb on - strand at 2308.594 kb on + strand, within Dsui_2172 at 2308.594 kb on + strand, within Dsui_2172 at 2308.849 kb on + strand, within Dsui_2172 at 2308.957 kb on + strand at 2308.970 kb on + strand at 2309.060 kb on + strand at 2309.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,305,596 - +1.0 2,305,687 - -0.5 2,306,214 + -2.9 2,306,305 + -2.9 2,306,407 - -1.4 2,306,500 + -0.7 2,306,838 - -1.8 2,306,883 - -1.4 2,306,938 + Dsui_2170 0.24 -1.4 2,306,963 + -3.5 2,307,021 + -2.4 2,307,091 - -1.5 2,307,109 - Dsui_2170 0.36 -2.2 2,307,109 - -2.1 2,307,470 + -1.5 2,307,470 + -0.6 2,307,470 + +1.2 2,307,532 + -0.9 2,307,594 + +0.6 2,307,891 + Dsui_2170 0.88 -1.2 2,308,009 + -0.6 2,308,017 - -1.8 2,308,113 - +0.0 2,308,129 + Dsui_2171 0.15 -1.7 2,308,135 + -3.1 2,308,392 + -0.7 2,308,409 - +0.6 2,308,594 + Dsui_2172 0.26 -1.4 2,308,594 + Dsui_2172 0.26 +1.3 2,308,849 + Dsui_2172 0.80 -0.5 2,308,957 + +0.8 2,308,970 + +0.7 2,309,060 + -1.0 2,309,060 + +0.1
Or see this region's nucleotide sequence