Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2085 and Dsui_2086 are separated by 226 nucleotides Dsui_2086 and Dsui_2087 are separated by 202 nucleotides Dsui_2087 and Dsui_2088 are separated by 36 nucleotides
Dsui_2085: Dsui_2085 - NADPH-dependent FMN reductase, at 2,205,698 to 2,207,074
_2085
Dsui_2086: Dsui_2086 - cytochrome c, at 2,207,301 to 2,209,391
_2086
Dsui_2087: Dsui_2087 - cytochrome c553, at 2,209,594 to 2,210,328
_2087
Dsui_2088: Dsui_2088 - cytochrome c, at 2,210,365 to 2,211,318
_2088
Position (kb)
2207
2208
2209
2210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2206.358 kb on - strand at 2206.554 kb on - strand at 2206.577 kb on - strand, within Dsui_2085 at 2206.668 kb on - strand at 2206.670 kb on - strand at 2206.787 kb on - strand at 2206.867 kb on + strand at 2207.257 kb on + strand at 2207.257 kb on + strand at 2207.257 kb on + strand at 2207.257 kb on + strand at 2207.257 kb on + strand at 2207.265 kb on - strand at 2207.279 kb on + strand at 2207.600 kb on + strand at 2207.659 kb on + strand, within Dsui_2086 at 2207.670 kb on + strand at 2207.675 kb on + strand at 2207.675 kb on + strand at 2207.678 kb on - strand, within Dsui_2086 at 2207.786 kb on - strand at 2207.817 kb on - strand at 2207.848 kb on - strand at 2207.849 kb on + strand at 2207.937 kb on + strand, within Dsui_2086 at 2208.039 kb on - strand, within Dsui_2086 at 2208.077 kb on + strand, within Dsui_2086 at 2208.085 kb on - strand at 2208.137 kb on + strand at 2208.200 kb on + strand, within Dsui_2086 at 2208.200 kb on + strand at 2208.400 kb on + strand at 2208.457 kb on + strand, within Dsui_2086 at 2208.499 kb on + strand at 2208.673 kb on - strand at 2208.679 kb on - strand at 2208.908 kb on + strand, within Dsui_2086 at 2209.069 kb on + strand at 2209.087 kb on - strand at 2209.187 kb on + strand at 2209.196 kb on - strand at 2209.268 kb on - strand at 2209.288 kb on + strand at 2209.592 kb on - strand at 2209.722 kb on - strand at 2209.908 kb on + strand at 2209.908 kb on + strand at 2209.908 kb on + strand at 2209.959 kb on + strand at 2210.143 kb on + strand, within Dsui_2087 at 2210.225 kb on + strand at 2210.247 kb on + strand, within Dsui_2087 at 2210.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,206,358 - +0.1 2,206,554 - -0.0 2,206,577 - Dsui_2085 0.64 -2.5 2,206,668 - -0.4 2,206,670 - -1.8 2,206,787 - +1.3 2,206,867 + -0.4 2,207,257 + -1.6 2,207,257 + +1.9 2,207,257 + -3.4 2,207,257 + +0.6 2,207,257 + -1.6 2,207,265 - -2.2 2,207,279 + -0.4 2,207,600 + +1.5 2,207,659 + Dsui_2086 0.17 -1.4 2,207,670 + -3.6 2,207,675 + -1.2 2,207,675 + -1.8 2,207,678 - Dsui_2086 0.18 -2.7 2,207,786 - -1.3 2,207,817 - -1.1 2,207,848 - -1.7 2,207,849 + -0.4 2,207,937 + Dsui_2086 0.30 +0.8 2,208,039 - Dsui_2086 0.35 -3.7 2,208,077 + Dsui_2086 0.37 -2.1 2,208,085 - -2.6 2,208,137 + -3.3 2,208,200 + Dsui_2086 0.43 -2.5 2,208,200 + -1.7 2,208,400 + -0.5 2,208,457 + Dsui_2086 0.55 -0.1 2,208,499 + -1.7 2,208,673 - +1.6 2,208,679 - -2.3 2,208,908 + Dsui_2086 0.77 -3.6 2,209,069 + -2.6 2,209,087 - -2.2 2,209,187 + +0.4 2,209,196 - -2.3 2,209,268 - -1.6 2,209,288 + -2.0 2,209,592 - -0.7 2,209,722 - +1.1 2,209,908 + +0.6 2,209,908 + -0.8 2,209,908 + -3.2 2,209,959 + +0.0 2,210,143 + Dsui_2087 0.75 +0.0 2,210,225 + -0.5 2,210,247 + Dsui_2087 0.89 +1.0 2,210,255 - -3.1
Or see this region's nucleotide sequence