Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1534 and Dsui_1535 are separated by 30 nucleotides Dsui_1535 and Dsui_1536 are separated by 116 nucleotides Dsui_1536 and Dsui_1537 are separated by 120 nucleotides Dsui_1537 and Dsui_1538 are separated by 47 nucleotides
Dsui_1534: Dsui_1534 - ribosomal protein L33, at 1,596,843 to 1,596,998
_1534
Dsui_1535: Dsui_1535 - ribosomal protein L28, at 1,597,029 to 1,597,262
_1535
Dsui_1536: Dsui_1536 - PilZ domain-containing protein, at 1,597,379 to 1,597,705
_1536
Dsui_1537: Dsui_1537 - DNA repair protein radc, at 1,597,826 to 1,598,503
_1537
Dsui_1538: Dsui_1538 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase, at 1,598,551 to 1,599,735
_1538
Position (kb)
1597
1598
1599 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1596.838 kb on - strand at 1596.936 kb on - strand, within Dsui_1534 at 1597.013 kb on - strand at 1597.384 kb on - strand at 1597.384 kb on - strand at 1597.436 kb on - strand, within Dsui_1536 at 1597.443 kb on - strand, within Dsui_1536 at 1597.447 kb on - strand, within Dsui_1536 at 1597.497 kb on + strand, within Dsui_1536 at 1597.520 kb on - strand, within Dsui_1536 at 1597.597 kb on + strand, within Dsui_1536 at 1597.597 kb on + strand at 1597.597 kb on + strand, within Dsui_1536 at 1597.604 kb on + strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.773 kb on + strand at 1597.788 kb on + strand at 1597.788 kb on + strand at 1597.796 kb on - strand at 1597.798 kb on - strand at 1597.798 kb on - strand at 1597.798 kb on - strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.827 kb on - strand at 1597.827 kb on - strand at 1597.832 kb on - strand at 1597.832 kb on - strand at 1597.832 kb on - strand at 1597.837 kb on - strand at 1597.911 kb on - strand, within Dsui_1537 at 1597.917 kb on - strand at 1597.927 kb on + strand at 1597.969 kb on + strand at 1598.214 kb on + strand, within Dsui_1537 at 1598.253 kb on + strand, within Dsui_1537 at 1598.261 kb on - strand, within Dsui_1537 at 1598.277 kb on - strand, within Dsui_1537 at 1598.337 kb on - strand, within Dsui_1537 at 1598.377 kb on + strand at 1598.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,596,838 - +1.2 1,596,936 - Dsui_1534 0.60 -0.7 1,597,013 - +0.2 1,597,384 - +1.2 1,597,384 - +1.3 1,597,436 - Dsui_1536 0.17 +1.0 1,597,443 - Dsui_1536 0.20 +0.9 1,597,447 - Dsui_1536 0.21 +0.8 1,597,497 + Dsui_1536 0.36 +0.1 1,597,520 - Dsui_1536 0.43 +1.6 1,597,597 + Dsui_1536 0.67 -2.6 1,597,597 + -3.7 1,597,597 + Dsui_1536 0.67 -0.9 1,597,604 + Dsui_1536 0.69 -0.9 1,597,605 - Dsui_1536 0.69 +0.5 1,597,605 - Dsui_1536 0.69 -0.2 1,597,605 - Dsui_1536 0.69 +0.3 1,597,605 - Dsui_1536 0.69 +0.3 1,597,605 - Dsui_1536 0.69 -0.2 1,597,773 + +0.0 1,597,788 + -2.2 1,597,788 + +0.0 1,597,796 - +1.7 1,597,798 - -2.6 1,597,798 - -0.2 1,597,798 - -1.3 1,597,824 + +1.1 1,597,824 + -0.3 1,597,824 + +0.3 1,597,824 + -2.1 1,597,824 + -3.6 1,597,824 + -1.8 1,597,827 - -1.7 1,597,827 - +0.1 1,597,832 - -2.4 1,597,832 - -0.8 1,597,832 - -1.7 1,597,837 - +0.0 1,597,911 - Dsui_1537 0.13 -1.2 1,597,917 - +0.1 1,597,927 + -0.1 1,597,969 + +1.1 1,598,214 + Dsui_1537 0.57 -1.3 1,598,253 + Dsui_1537 0.63 -1.8 1,598,261 - Dsui_1537 0.64 -1.7 1,598,277 - Dsui_1537 0.67 -1.5 1,598,337 - Dsui_1537 0.75 -2.8 1,598,377 + +0.9 1,598,511 + +0.7
Or see this region's nucleotide sequence