Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1532 and Dsui_1533 are separated by 76 nucleotides Dsui_1533 and Dsui_1534 are separated by 376 nucleotides Dsui_1534 and Dsui_1535 are separated by 30 nucleotides Dsui_1535 and Dsui_1536 are separated by 116 nucleotides
Dsui_1532: Dsui_1532 - fatty-acid desaturase, at 1,594,684 to 1,595,898
_1532
Dsui_1533: Dsui_1533 - disulfide bond formation protein DsbB, at 1,595,975 to 1,596,466
_1533
Dsui_1534: Dsui_1534 - ribosomal protein L33, at 1,596,843 to 1,596,998
_1534
Dsui_1535: Dsui_1535 - ribosomal protein L28, at 1,597,029 to 1,597,262
_1535
Dsui_1536: Dsui_1536 - PilZ domain-containing protein, at 1,597,379 to 1,597,705
_1536
Position (kb)
1595
1596
1597 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1594.983 kb on + strand, within Dsui_1532 at 1594.983 kb on + strand, within Dsui_1532 at 1595.033 kb on - strand, within Dsui_1532 at 1595.191 kb on + strand, within Dsui_1532 at 1595.250 kb on + strand, within Dsui_1532 at 1595.250 kb on + strand, within Dsui_1532 at 1595.258 kb on - strand, within Dsui_1532 at 1595.294 kb on + strand, within Dsui_1532 at 1595.332 kb on - strand, within Dsui_1532 at 1595.392 kb on - strand, within Dsui_1532 at 1595.429 kb on + strand, within Dsui_1532 at 1595.442 kb on - strand, within Dsui_1532 at 1595.501 kb on + strand, within Dsui_1532 at 1595.579 kb on + strand, within Dsui_1532 at 1595.592 kb on + strand, within Dsui_1532 at 1595.757 kb on + strand, within Dsui_1532 at 1595.775 kb on - strand, within Dsui_1532 at 1595.796 kb on - strand at 1595.921 kb on - strand at 1595.953 kb on + strand at 1596.046 kb on - strand, within Dsui_1533 at 1596.056 kb on - strand at 1596.153 kb on - strand at 1596.183 kb on - strand, within Dsui_1533 at 1596.224 kb on + strand, within Dsui_1533 at 1596.237 kb on - strand, within Dsui_1533 at 1596.263 kb on + strand, within Dsui_1533 at 1596.263 kb on + strand, within Dsui_1533 at 1596.271 kb on - strand at 1596.370 kb on + strand, within Dsui_1533 at 1596.373 kb on - strand, within Dsui_1533 at 1596.379 kb on - strand at 1596.525 kb on + strand at 1596.530 kb on + strand at 1596.838 kb on - strand at 1596.936 kb on - strand, within Dsui_1534 at 1597.013 kb on - strand at 1597.384 kb on - strand at 1597.384 kb on - strand at 1597.436 kb on - strand, within Dsui_1536 at 1597.443 kb on - strand, within Dsui_1536 at 1597.447 kb on - strand, within Dsui_1536
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,594,983 + Dsui_1532 0.25 -0.0 1,594,983 + Dsui_1532 0.25 +0.2 1,595,033 - Dsui_1532 0.29 -0.2 1,595,191 + Dsui_1532 0.42 +0.5 1,595,250 + Dsui_1532 0.47 -0.1 1,595,250 + Dsui_1532 0.47 +0.4 1,595,258 - Dsui_1532 0.47 -0.5 1,595,294 + Dsui_1532 0.50 -0.2 1,595,332 - Dsui_1532 0.53 -0.5 1,595,392 - Dsui_1532 0.58 -0.4 1,595,429 + Dsui_1532 0.61 +0.2 1,595,442 - Dsui_1532 0.62 -1.3 1,595,501 + Dsui_1532 0.67 -2.3 1,595,579 + Dsui_1532 0.74 +0.2 1,595,592 + Dsui_1532 0.75 +0.1 1,595,757 + Dsui_1532 0.88 +0.7 1,595,775 - Dsui_1532 0.90 -0.3 1,595,796 - -0.2 1,595,921 - -1.0 1,595,953 + +0.2 1,596,046 - Dsui_1533 0.14 -2.7 1,596,056 - -0.8 1,596,153 - -2.9 1,596,183 - Dsui_1533 0.42 -1.6 1,596,224 + Dsui_1533 0.51 -2.2 1,596,237 - Dsui_1533 0.53 -3.9 1,596,263 + Dsui_1533 0.59 -2.2 1,596,263 + Dsui_1533 0.59 -4.8 1,596,271 - -0.8 1,596,370 + Dsui_1533 0.80 -3.6 1,596,373 - Dsui_1533 0.81 -2.6 1,596,379 - -3.0 1,596,525 + -0.2 1,596,530 + -1.7 1,596,838 - +1.2 1,596,936 - Dsui_1534 0.60 -0.7 1,597,013 - +0.2 1,597,384 - +1.2 1,597,384 - +1.3 1,597,436 - Dsui_1536 0.17 +1.0 1,597,443 - Dsui_1536 0.20 +0.9 1,597,447 - Dsui_1536 0.21 +0.8
Or see this region's nucleotide sequence