Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1485 and Dsui_1488 are separated by 734 nucleotides Dsui_1488 and Dsui_1489 are separated by 28 nucleotides
Dsui_1485: Dsui_1485 - NC domain protein, at 1,549,537 to 1,549,905
_1485
Dsui_1488: Dsui_1488 - putative metal-dependent hydrolase, at 1,550,640 to 1,551,002
_1488
Dsui_1489: Dsui_1489 - hypothetical protein, at 1,551,031 to 1,552,383
_1489
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1549.757 kb on - strand, within Dsui_1485 at 1549.851 kb on + strand, within Dsui_1485 at 1549.893 kb on - strand at 1549.976 kb on + strand at 1550.007 kb on - strand at 1550.034 kb on - strand at 1550.072 kb on + strand at 1550.140 kb on - strand at 1550.184 kb on + strand at 1550.221 kb on - strand at 1550.273 kb on - strand at 1550.278 kb on - strand at 1550.411 kb on + strand at 1550.419 kb on - strand at 1550.690 kb on - strand, within Dsui_1488 at 1550.822 kb on + strand, within Dsui_1488 at 1550.894 kb on - strand, within Dsui_1488 at 1550.911 kb on - strand, within Dsui_1488 at 1550.911 kb on - strand, within Dsui_1488 at 1550.920 kb on + strand, within Dsui_1488 at 1550.920 kb on + strand, within Dsui_1488 at 1551.041 kb on - strand at 1551.091 kb on + strand at 1551.218 kb on - strand, within Dsui_1489 at 1551.315 kb on - strand, within Dsui_1489 at 1551.400 kb on - strand, within Dsui_1489 at 1551.479 kb on - strand, within Dsui_1489 at 1551.535 kb on - strand at 1551.596 kb on + strand, within Dsui_1489 at 1551.660 kb on + strand, within Dsui_1489 at 1551.660 kb on + strand, within Dsui_1489 at 1551.698 kb on + strand, within Dsui_1489 at 1551.799 kb on - strand, within Dsui_1489 at 1551.809 kb on - strand, within Dsui_1489 at 1551.809 kb on - strand, within Dsui_1489 at 1551.934 kb on + strand, within Dsui_1489 at 1551.949 kb on + strand, within Dsui_1489
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,549,757 - Dsui_1485 0.60 -1.0 1,549,851 + Dsui_1485 0.85 +0.6 1,549,893 - -0.5 1,549,976 + +0.0 1,550,007 - -0.6 1,550,034 - -4.1 1,550,072 + +0.1 1,550,140 - -0.6 1,550,184 + -1.2 1,550,221 - +0.6 1,550,273 - +0.9 1,550,278 - -0.4 1,550,411 + +0.7 1,550,419 - +1.6 1,550,690 - Dsui_1488 0.14 +0.3 1,550,822 + Dsui_1488 0.50 +0.1 1,550,894 - Dsui_1488 0.70 -2.1 1,550,911 - Dsui_1488 0.75 -2.1 1,550,911 - Dsui_1488 0.75 +0.9 1,550,920 + Dsui_1488 0.77 -0.0 1,550,920 + Dsui_1488 0.77 +1.3 1,551,041 - +0.1 1,551,091 + +0.0 1,551,218 - Dsui_1489 0.14 +0.0 1,551,315 - Dsui_1489 0.21 +0.8 1,551,400 - Dsui_1489 0.27 -1.3 1,551,479 - Dsui_1489 0.33 -0.1 1,551,535 - -1.1 1,551,596 + Dsui_1489 0.42 -2.2 1,551,660 + Dsui_1489 0.46 -1.3 1,551,660 + Dsui_1489 0.46 +1.2 1,551,698 + Dsui_1489 0.49 -0.6 1,551,799 - Dsui_1489 0.57 +0.9 1,551,809 - Dsui_1489 0.58 -0.7 1,551,809 - Dsui_1489 0.58 +1.0 1,551,934 + Dsui_1489 0.67 +1.7 1,551,949 + Dsui_1489 0.68 +0.2
Or see this region's nucleotide sequence