Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1361 and Dsui_1362 are separated by 1 nucleotides Dsui_1362 and Dsui_1363 are separated by 12 nucleotides Dsui_1363 and Dsui_1364 are separated by 23 nucleotides Dsui_1364 and Dsui_1365 are separated by 15 nucleotides Dsui_1365 and Dsui_1366 are separated by 36 nucleotides
Dsui_1361: Dsui_1361 - diguanylate cyclase (GGDEF) domain-containing protein, at 1,415,725 to 1,416,501
_1361
Dsui_1362: Dsui_1362 - protein of unknown function (DUF1987), at 1,416,503 to 1,416,880
_1362
Dsui_1363: Dsui_1363 - hypothetical protein, at 1,416,893 to 1,417,441
_1363
Dsui_1364: Dsui_1364 - serine phosphatase RsbU, regulator of sigma subunit, at 1,417,465 to 1,419,462
_1364
Dsui_1365: Dsui_1365 - hypothetical protein, at 1,419,478 to 1,419,693
_1365
Dsui_1366: Dsui_1366 - glutaminyl-tRNA synthetase, at 1,419,730 to 1,421,511
_1366
Position (kb)
1417
1418
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1416.643 kb on + strand, within Dsui_1362 at 1416.651 kb on - strand, within Dsui_1362 at 1416.651 kb on - strand, within Dsui_1362 at 1416.651 kb on - strand, within Dsui_1362 at 1416.651 kb on - strand, within Dsui_1362 at 1416.723 kb on + strand, within Dsui_1362 at 1416.745 kb on - strand, within Dsui_1362 at 1416.758 kb on + strand, within Dsui_1362 at 1416.759 kb on + strand, within Dsui_1362 at 1416.767 kb on - strand, within Dsui_1362 at 1416.792 kb on - strand, within Dsui_1362 at 1416.862 kb on - strand at 1416.873 kb on - strand at 1416.997 kb on + strand, within Dsui_1363 at 1417.018 kb on - strand, within Dsui_1363 at 1417.018 kb on - strand, within Dsui_1363 at 1417.044 kb on - strand, within Dsui_1363 at 1417.095 kb on - strand, within Dsui_1363 at 1417.158 kb on - strand, within Dsui_1363 at 1417.198 kb on + strand, within Dsui_1363 at 1417.212 kb on - strand, within Dsui_1363 at 1417.219 kb on + strand, within Dsui_1363 at 1417.266 kb on - strand, within Dsui_1363 at 1417.299 kb on + strand, within Dsui_1363 at 1417.313 kb on + strand, within Dsui_1363 at 1417.321 kb on - strand, within Dsui_1363 at 1417.321 kb on - strand, within Dsui_1363 at 1417.321 kb on - strand, within Dsui_1363 at 1417.403 kb on - strand at 1417.468 kb on + strand at 1417.597 kb on + strand at 1417.628 kb on + strand at 1417.636 kb on - strand at 1417.643 kb on + strand at 1417.670 kb on - strand, within Dsui_1364 at 1417.677 kb on + strand at 1417.677 kb on + strand, within Dsui_1364 at 1417.677 kb on + strand, within Dsui_1364 at 1417.685 kb on - strand, within Dsui_1364 at 1417.685 kb on - strand, within Dsui_1364 at 1417.685 kb on - strand, within Dsui_1364 at 1417.685 kb on - strand, within Dsui_1364 at 1417.860 kb on - strand, within Dsui_1364 at 1417.863 kb on + strand, within Dsui_1364 at 1417.866 kb on - strand, within Dsui_1364 at 1418.075 kb on + strand, within Dsui_1364 at 1418.134 kb on + strand, within Dsui_1364 at 1418.153 kb on + strand, within Dsui_1364 at 1418.230 kb on + strand, within Dsui_1364 at 1418.241 kb on + strand, within Dsui_1364 at 1418.241 kb on + strand, within Dsui_1364 at 1418.244 kb on - strand, within Dsui_1364 at 1418.249 kb on - strand, within Dsui_1364 at 1418.326 kb on - strand, within Dsui_1364 at 1418.466 kb on - strand, within Dsui_1364 at 1418.483 kb on + strand, within Dsui_1364 at 1418.534 kb on + strand, within Dsui_1364 at 1418.569 kb on - strand, within Dsui_1364 at 1418.617 kb on - strand, within Dsui_1364 at 1418.664 kb on + strand, within Dsui_1364 at 1418.672 kb on - strand, within Dsui_1364 at 1418.780 kb on - strand, within Dsui_1364 at 1418.816 kb on - strand, within Dsui_1364 at 1418.906 kb on + strand, within Dsui_1364 at 1418.906 kb on + strand at 1418.978 kb on + strand, within Dsui_1364 at 1419.049 kb on - strand, within Dsui_1364 at 1419.127 kb on - strand, within Dsui_1364 at 1419.159 kb on - strand, within Dsui_1364 at 1419.179 kb on + strand, within Dsui_1364 at 1419.289 kb on + strand at 1419.310 kb on + strand at 1419.340 kb on + strand at 1419.497 kb on - strand at 1419.605 kb on + strand at 1419.673 kb on + strand at 1419.676 kb on - strand at 1419.727 kb on + strand at 1419.751 kb on - strand at 1419.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,416,643 + Dsui_1362 0.37 -1.5 1,416,651 - Dsui_1362 0.39 +0.8 1,416,651 - Dsui_1362 0.39 -0.2 1,416,651 - Dsui_1362 0.39 +0.5 1,416,651 - Dsui_1362 0.39 -0.4 1,416,723 + Dsui_1362 0.58 -0.2 1,416,745 - Dsui_1362 0.64 +0.4 1,416,758 + Dsui_1362 0.67 -0.2 1,416,759 + Dsui_1362 0.68 +1.1 1,416,767 - Dsui_1362 0.70 -0.5 1,416,792 - Dsui_1362 0.76 -0.4 1,416,862 - +0.1 1,416,873 - -0.3 1,416,997 + Dsui_1363 0.19 +0.1 1,417,018 - Dsui_1363 0.23 -0.4 1,417,018 - Dsui_1363 0.23 +1.2 1,417,044 - Dsui_1363 0.28 +0.8 1,417,095 - Dsui_1363 0.37 +1.2 1,417,158 - Dsui_1363 0.48 +0.5 1,417,198 + Dsui_1363 0.56 -0.2 1,417,212 - Dsui_1363 0.58 +0.7 1,417,219 + Dsui_1363 0.59 +0.2 1,417,266 - Dsui_1363 0.68 +0.6 1,417,299 + Dsui_1363 0.74 -1.3 1,417,313 + Dsui_1363 0.77 -0.3 1,417,321 - Dsui_1363 0.78 +0.5 1,417,321 - Dsui_1363 0.78 +0.2 1,417,321 - Dsui_1363 0.78 +0.8 1,417,403 - +1.4 1,417,468 + +0.6 1,417,597 + -1.1 1,417,628 + -1.7 1,417,636 - -2.4 1,417,643 + -1.1 1,417,670 - Dsui_1364 0.10 -0.1 1,417,677 + -0.0 1,417,677 + Dsui_1364 0.11 -0.5 1,417,677 + Dsui_1364 0.11 +0.7 1,417,685 - Dsui_1364 0.11 -0.2 1,417,685 - Dsui_1364 0.11 +0.8 1,417,685 - Dsui_1364 0.11 +0.0 1,417,685 - Dsui_1364 0.11 +2.0 1,417,860 - Dsui_1364 0.20 +1.0 1,417,863 + Dsui_1364 0.20 -0.3 1,417,866 - Dsui_1364 0.20 +0.6 1,418,075 + Dsui_1364 0.31 -0.2 1,418,134 + Dsui_1364 0.33 -0.3 1,418,153 + Dsui_1364 0.34 -0.9 1,418,230 + Dsui_1364 0.38 -0.1 1,418,241 + Dsui_1364 0.39 -1.7 1,418,241 + Dsui_1364 0.39 -0.4 1,418,244 - Dsui_1364 0.39 -0.8 1,418,249 - Dsui_1364 0.39 +0.6 1,418,326 - Dsui_1364 0.43 -0.0 1,418,466 - Dsui_1364 0.50 -0.6 1,418,483 + Dsui_1364 0.51 -0.6 1,418,534 + Dsui_1364 0.54 +0.2 1,418,569 - Dsui_1364 0.55 +0.2 1,418,617 - Dsui_1364 0.58 +1.4 1,418,664 + Dsui_1364 0.60 -2.1 1,418,672 - Dsui_1364 0.60 -2.1 1,418,780 - Dsui_1364 0.66 +0.1 1,418,816 - Dsui_1364 0.68 -2.0 1,418,906 + Dsui_1364 0.72 -0.2 1,418,906 + -0.2 1,418,978 + Dsui_1364 0.76 -0.0 1,419,049 - Dsui_1364 0.79 +0.8 1,419,127 - Dsui_1364 0.83 -0.2 1,419,159 - Dsui_1364 0.85 +0.5 1,419,179 + Dsui_1364 0.86 -0.8 1,419,289 + -1.2 1,419,310 + +0.0 1,419,340 + -0.0 1,419,497 - -0.1 1,419,605 + +0.0 1,419,673 + -0.3 1,419,676 - +1.4 1,419,727 + +0.0 1,419,751 - +0.4 1,419,751 - -2.9
Or see this region's nucleotide sequence