Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1347 and Dsui_1348 are separated by 162 nucleotides Dsui_1348 and Dsui_1349 are separated by 50 nucleotides Dsui_1349 and Dsui_1350 are separated by 135 nucleotides Dsui_1350 and Dsui_1351 are separated by 184 nucleotides
Dsui_1347: Dsui_1347 - HD-GYP domain-containing protein, at 1,403,693 to 1,404,799
_1347
Dsui_1348: Dsui_1348 - hemerythrin-like metal-binding domain-containing protein, at 1,404,962 to 1,405,372
_1348
Dsui_1349: Dsui_1349 - hypothetical protein, at 1,405,423 to 1,405,632
_1349
Dsui_1350: Dsui_1350 - hypothetical protein, at 1,405,768 to 1,405,977
_1350
Dsui_1351: Dsui_1351 - DNA/RNA helicase, superfamily II, at 1,406,162 to 1,408,087
_1351
Position (kb)
1404
1405
1406 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1403.973 kb on - strand, within Dsui_1347 at 1403.990 kb on - strand, within Dsui_1347 at 1404.042 kb on + strand, within Dsui_1347 at 1404.055 kb on + strand, within Dsui_1347 at 1404.062 kb on - strand, within Dsui_1347 at 1404.062 kb on - strand, within Dsui_1347 at 1404.063 kb on - strand, within Dsui_1347 at 1404.114 kb on + strand, within Dsui_1347 at 1404.137 kb on - strand, within Dsui_1347 at 1404.179 kb on - strand, within Dsui_1347 at 1404.179 kb on - strand, within Dsui_1347 at 1404.181 kb on + strand, within Dsui_1347 at 1404.181 kb on + strand, within Dsui_1347 at 1404.181 kb on + strand, within Dsui_1347 at 1404.181 kb on + strand, within Dsui_1347 at 1404.261 kb on - strand, within Dsui_1347 at 1404.268 kb on - strand, within Dsui_1347 at 1404.278 kb on + strand, within Dsui_1347 at 1404.288 kb on + strand, within Dsui_1347 at 1404.327 kb on + strand, within Dsui_1347 at 1404.360 kb on + strand, within Dsui_1347 at 1404.363 kb on - strand, within Dsui_1347 at 1404.365 kb on + strand, within Dsui_1347 at 1404.368 kb on - strand, within Dsui_1347 at 1404.368 kb on - strand, within Dsui_1347 at 1404.478 kb on + strand at 1404.486 kb on - strand, within Dsui_1347 at 1404.496 kb on - strand, within Dsui_1347 at 1404.608 kb on - strand, within Dsui_1347 at 1404.618 kb on - strand, within Dsui_1347 at 1404.623 kb on + strand, within Dsui_1347 at 1404.629 kb on + strand, within Dsui_1347 at 1404.666 kb on - strand, within Dsui_1347 at 1404.671 kb on - strand, within Dsui_1347 at 1404.719 kb on - strand at 1404.725 kb on + strand at 1404.805 kb on + strand at 1404.832 kb on + strand at 1404.844 kb on - strand at 1404.846 kb on + strand at 1404.849 kb on - strand at 1404.857 kb on - strand at 1404.919 kb on + strand at 1404.953 kb on + strand at 1404.973 kb on + strand at 1404.980 kb on - strand at 1404.986 kb on - strand at 1404.992 kb on + strand at 1404.999 kb on + strand at 1405.005 kb on - strand at 1405.013 kb on + strand, within Dsui_1348 at 1405.015 kb on - strand, within Dsui_1348 at 1405.020 kb on + strand, within Dsui_1348 at 1405.021 kb on - strand, within Dsui_1348 at 1405.050 kb on - strand, within Dsui_1348 at 1405.145 kb on - strand, within Dsui_1348 at 1405.204 kb on - strand, within Dsui_1348 at 1405.208 kb on + strand, within Dsui_1348 at 1405.290 kb on + strand, within Dsui_1348 at 1405.321 kb on + strand, within Dsui_1348 at 1405.342 kb on + strand at 1405.456 kb on + strand, within Dsui_1349 at 1405.492 kb on - strand, within Dsui_1349 at 1405.494 kb on + strand, within Dsui_1349 at 1405.530 kb on + strand, within Dsui_1349 at 1405.565 kb on - strand, within Dsui_1349 at 1405.573 kb on + strand, within Dsui_1349 at 1405.676 kb on + strand at 1405.740 kb on + strand at 1405.740 kb on + strand at 1405.762 kb on + strand at 1405.763 kb on + strand at 1405.770 kb on - strand at 1405.771 kb on - strand at 1405.818 kb on - strand at 1405.881 kb on - strand, within Dsui_1350 at 1405.989 kb on - strand at 1406.075 kb on + strand at 1406.112 kb on + strand at 1406.117 kb on + strand at 1406.119 kb on - strand at 1406.127 kb on + strand at 1406.127 kb on + strand at 1406.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,403,973 - Dsui_1347 0.25 +0.3 1,403,990 - Dsui_1347 0.27 +0.5 1,404,042 + Dsui_1347 0.32 -1.6 1,404,055 + Dsui_1347 0.33 +1.0 1,404,062 - Dsui_1347 0.33 +0.1 1,404,062 - Dsui_1347 0.33 +1.0 1,404,063 - Dsui_1347 0.33 -1.6 1,404,114 + Dsui_1347 0.38 +0.4 1,404,137 - Dsui_1347 0.40 +0.3 1,404,179 - Dsui_1347 0.44 +0.8 1,404,179 - Dsui_1347 0.44 +0.2 1,404,181 + Dsui_1347 0.44 +0.0 1,404,181 + Dsui_1347 0.44 -0.5 1,404,181 + Dsui_1347 0.44 +0.8 1,404,181 + Dsui_1347 0.44 +0.6 1,404,261 - Dsui_1347 0.51 +0.2 1,404,268 - Dsui_1347 0.52 +1.0 1,404,278 + Dsui_1347 0.53 -0.6 1,404,288 + Dsui_1347 0.54 +0.0 1,404,327 + Dsui_1347 0.57 +0.4 1,404,360 + Dsui_1347 0.60 +0.3 1,404,363 - Dsui_1347 0.61 +2.1 1,404,365 + Dsui_1347 0.61 +0.4 1,404,368 - Dsui_1347 0.61 +1.2 1,404,368 - Dsui_1347 0.61 +0.8 1,404,478 + +0.3 1,404,486 - Dsui_1347 0.72 +1.6 1,404,496 - Dsui_1347 0.73 +0.5 1,404,608 - Dsui_1347 0.83 +0.3 1,404,618 - Dsui_1347 0.84 +0.6 1,404,623 + Dsui_1347 0.84 -0.1 1,404,629 + Dsui_1347 0.85 +0.7 1,404,666 - Dsui_1347 0.88 -0.7 1,404,671 - Dsui_1347 0.88 +0.1 1,404,719 - -0.0 1,404,725 + -2.7 1,404,805 + -1.7 1,404,832 + +0.7 1,404,844 - -1.3 1,404,846 + -1.1 1,404,849 - -1.9 1,404,857 - -2.1 1,404,919 + -0.5 1,404,953 + -0.3 1,404,973 + -2.9 1,404,980 - -5.4 1,404,986 - -2.1 1,404,992 + -2.6 1,404,999 + -4.9 1,405,005 - -2.0 1,405,013 + Dsui_1348 0.12 -4.3 1,405,015 - Dsui_1348 0.13 -2.2 1,405,020 + Dsui_1348 0.14 -2.4 1,405,021 - Dsui_1348 0.14 -3.9 1,405,050 - Dsui_1348 0.21 -2.8 1,405,145 - Dsui_1348 0.45 -2.9 1,405,204 - Dsui_1348 0.59 -2.6 1,405,208 + Dsui_1348 0.60 -1.2 1,405,290 + Dsui_1348 0.80 -3.3 1,405,321 + Dsui_1348 0.87 -2.3 1,405,342 + -0.1 1,405,456 + Dsui_1349 0.16 +0.2 1,405,492 - Dsui_1349 0.33 -0.2 1,405,494 + Dsui_1349 0.34 -0.1 1,405,530 + Dsui_1349 0.51 +0.4 1,405,565 - Dsui_1349 0.68 -1.0 1,405,573 + Dsui_1349 0.71 -0.2 1,405,676 + -1.3 1,405,740 + +0.9 1,405,740 + +1.4 1,405,762 + +0.3 1,405,763 + +1.2 1,405,770 - +0.1 1,405,771 - +1.2 1,405,818 - -2.5 1,405,881 - Dsui_1350 0.54 -1.9 1,405,989 - -0.2 1,406,075 + +1.0 1,406,112 + +1.7 1,406,117 + +1.9 1,406,119 - -2.8 1,406,127 + +0.8 1,406,127 + +0.4 1,406,174 + +0.4
Or see this region's nucleotide sequence