Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1194 and Dsui_1195 overlap by 1 nucleotides Dsui_1195 and Dsui_1196 overlap by 4 nucleotides Dsui_1196 and Dsui_1197 are separated by 60 nucleotides
Dsui_1194: Dsui_1194 - tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB, at 1,246,200 to 1,247,540
_1194
Dsui_1195: Dsui_1195 - phosphate starvation-inducible protein PhoH, predicted ATPase, at 1,247,540 to 1,248,514
_1195
Dsui_1196: Dsui_1196 - metalloprotein, YbeY/UPF0054 family, at 1,248,511 to 1,248,969
_1196
Dsui_1197: Dsui_1197 - putative Mg2+ and Co2+ transporter CorC, at 1,249,030 to 1,249,875
_1197
Position (kb) 1247
1248
1249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1246.616 kb on + strand, within Dsui_1194 at 1246.624 kb on - strand at 1246.628 kb on + strand, within Dsui_1194 at 1246.636 kb on - strand, within Dsui_1194 at 1246.782 kb on - strand, within Dsui_1194 at 1246.899 kb on + strand, within Dsui_1194 at 1246.909 kb on + strand, within Dsui_1194 at 1246.917 kb on - strand, within Dsui_1194 at 1246.917 kb on - strand, within Dsui_1194 at 1246.951 kb on + strand, within Dsui_1194 at 1246.980 kb on - strand, within Dsui_1194 at 1246.986 kb on - strand, within Dsui_1194 at 1246.992 kb on + strand, within Dsui_1194 at 1246.997 kb on + strand, within Dsui_1194 at 1247.002 kb on + strand, within Dsui_1194 at 1247.072 kb on + strand, within Dsui_1194 at 1247.104 kb on + strand, within Dsui_1194 at 1247.183 kb on - strand, within Dsui_1194 at 1247.213 kb on - strand, within Dsui_1194 at 1247.354 kb on - strand at 1247.409 kb on + strand at 1247.468 kb on + strand at 1247.608 kb on + strand at 1247.646 kb on + strand, within Dsui_1195 at 1247.713 kb on + strand, within Dsui_1195 at 1247.921 kb on - strand, within Dsui_1195 at 1247.922 kb on - strand, within Dsui_1195 at 1247.923 kb on - strand, within Dsui_1195 at 1247.969 kb on + strand, within Dsui_1195 at 1248.016 kb on + strand, within Dsui_1195 at 1248.026 kb on - strand, within Dsui_1195 at 1248.035 kb on + strand, within Dsui_1195 at 1248.222 kb on + strand, within Dsui_1195 at 1248.257 kb on - strand, within Dsui_1195 at 1248.268 kb on - strand, within Dsui_1195 at 1248.268 kb on - strand, within Dsui_1195 at 1248.358 kb on + strand, within Dsui_1195 at 1248.361 kb on - strand, within Dsui_1195 at 1248.410 kb on + strand, within Dsui_1195 at 1248.479 kb on + strand at 1248.515 kb on - strand at 1249.028 kb on - strand at 1249.058 kb on - strand at 1249.073 kb on - strand at 1249.082 kb on + strand at 1249.094 kb on - strand at 1249.196 kb on - strand, within Dsui_1197 at 1249.286 kb on - strand, within Dsui_1197 at 1249.369 kb on + strand, within Dsui_1197 at 1249.377 kb on - strand, within Dsui_1197 at 1249.394 kb on - strand, within Dsui_1197
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,246,616 + Dsui_1194 0.31 +0.2 1,246,624 - -0.6 1,246,628 + Dsui_1194 0.32 +1.1 1,246,636 - Dsui_1194 0.33 -3.1 1,246,782 - Dsui_1194 0.43 -0.2 1,246,899 + Dsui_1194 0.52 -0.1 1,246,909 + Dsui_1194 0.53 -0.3 1,246,917 - Dsui_1194 0.53 -2.7 1,246,917 - Dsui_1194 0.53 -3.3 1,246,951 + Dsui_1194 0.56 -1.2 1,246,980 - Dsui_1194 0.58 -1.3 1,246,986 - Dsui_1194 0.59 +0.3 1,246,992 + Dsui_1194 0.59 +1.4 1,246,997 + Dsui_1194 0.59 -1.1 1,247,002 + Dsui_1194 0.60 +0.1 1,247,072 + Dsui_1194 0.65 -0.8 1,247,104 + Dsui_1194 0.67 -0.2 1,247,183 - Dsui_1194 0.73 +0.9 1,247,213 - Dsui_1194 0.76 +0.7 1,247,354 - -3.4 1,247,409 + -0.9 1,247,468 + -0.3 1,247,608 + +2.2 1,247,646 + Dsui_1195 0.11 +0.3 1,247,713 + Dsui_1195 0.18 +1.1 1,247,921 - Dsui_1195 0.39 +1.4 1,247,922 - Dsui_1195 0.39 +1.3 1,247,923 - Dsui_1195 0.39 +0.6 1,247,969 + Dsui_1195 0.44 +1.0 1,248,016 + Dsui_1195 0.49 +0.9 1,248,026 - Dsui_1195 0.50 +1.2 1,248,035 + Dsui_1195 0.51 +0.2 1,248,222 + Dsui_1195 0.70 +1.6 1,248,257 - Dsui_1195 0.74 +1.2 1,248,268 - Dsui_1195 0.75 -0.2 1,248,268 - Dsui_1195 0.75 +0.4 1,248,358 + Dsui_1195 0.84 +1.4 1,248,361 - Dsui_1195 0.84 +0.6 1,248,410 + Dsui_1195 0.89 +0.9 1,248,479 + +0.6 1,248,515 - +2.1 1,249,028 - -0.6 1,249,058 - +0.2 1,249,073 - -1.4 1,249,082 + +0.9 1,249,094 - -1.3 1,249,196 - Dsui_1197 0.20 +0.1 1,249,286 - Dsui_1197 0.30 +0.1 1,249,369 + Dsui_1197 0.40 +0.2 1,249,377 - Dsui_1197 0.41 -1.1 1,249,394 - Dsui_1197 0.43 -2.0
Or see this region's nucleotide sequence