Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0816 and Dsui_0817 are separated by 55 nucleotides Dsui_0817 and Dsui_0818 are separated by 117 nucleotides Dsui_0818 and Dsui_0819 are separated by 9 nucleotides
Dsui_0816: Dsui_0816 - methionyl-tRNA formyltransferase, at 827,681 to 828,607
_0816
Dsui_0817: Dsui_0817 - peptide deformylase, at 828,663 to 829,166
_0817
Dsui_0818: Dsui_0818 - LysM domain-containing protein, at 829,284 to 830,339
_0818
Dsui_0819: Dsui_0819 - DNA protecting protein DprA, at 830,349 to 831,467
_0819
Position (kb) 829
830
831 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 828.639 kb on + strand at 828.639 kb on + strand at 828.639 kb on + strand at 828.639 kb on - strand at 828.639 kb on - strand at 828.639 kb on - strand at 828.644 kb on + strand at 828.644 kb on - strand at 828.647 kb on - strand at 828.647 kb on - strand at 828.652 kb on - strand at 829.151 kb on - strand at 829.155 kb on + strand at 829.163 kb on - strand at 829.189 kb on + strand at 829.225 kb on + strand at 829.225 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.232 kb on + strand at 829.235 kb on - strand at 829.240 kb on - strand at 829.240 kb on - strand at 829.240 kb on - strand at 829.240 kb on - strand at 829.240 kb on - strand at 829.266 kb on - strand at 829.266 kb on - strand at 829.266 kb on - strand at 829.311 kb on + strand at 829.311 kb on + strand at 829.311 kb on + strand at 829.311 kb on + strand at 829.311 kb on + strand at 829.311 kb on + strand at 829.319 kb on - strand at 829.319 kb on - strand at 829.319 kb on - strand at 829.319 kb on - strand at 829.343 kb on + strand at 829.382 kb on - strand at 829.404 kb on - strand, within Dsui_0818 at 829.438 kb on - strand, within Dsui_0818 at 829.473 kb on + strand, within Dsui_0818 at 829.473 kb on + strand, within Dsui_0818 at 829.481 kb on - strand, within Dsui_0818 at 829.481 kb on - strand, within Dsui_0818 at 829.574 kb on + strand, within Dsui_0818 at 829.620 kb on + strand, within Dsui_0818 at 829.628 kb on + strand, within Dsui_0818 at 829.628 kb on - strand, within Dsui_0818 at 829.677 kb on - strand, within Dsui_0818 at 829.681 kb on - strand, within Dsui_0818 at 829.696 kb on - strand, within Dsui_0818 at 829.702 kb on - strand, within Dsui_0818 at 829.709 kb on + strand, within Dsui_0818 at 829.768 kb on - strand, within Dsui_0818 at 829.792 kb on - strand, within Dsui_0818 at 829.792 kb on - strand, within Dsui_0818 at 829.792 kb on - strand, within Dsui_0818 at 829.792 kb on - strand, within Dsui_0818 at 829.806 kb on - strand, within Dsui_0818 at 829.806 kb on - strand, within Dsui_0818 at 829.852 kb on - strand, within Dsui_0818 at 829.852 kb on - strand, within Dsui_0818 at 829.863 kb on - strand, within Dsui_0818 at 829.864 kb on + strand, within Dsui_0818 at 829.880 kb on - strand, within Dsui_0818 at 829.880 kb on - strand, within Dsui_0818 at 829.887 kb on + strand, within Dsui_0818 at 829.911 kb on - strand, within Dsui_0818 at 829.911 kb on - strand, within Dsui_0818 at 830.014 kb on + strand, within Dsui_0818 at 830.015 kb on - strand, within Dsui_0818 at 830.017 kb on - strand, within Dsui_0818 at 830.043 kb on + strand, within Dsui_0818 at 830.050 kb on - strand, within Dsui_0818 at 830.064 kb on + strand, within Dsui_0818 at 830.064 kb on + strand, within Dsui_0818 at 830.072 kb on - strand, within Dsui_0818 at 830.103 kb on - strand, within Dsui_0818 at 830.103 kb on - strand, within Dsui_0818 at 830.103 kb on - strand, within Dsui_0818 at 830.111 kb on + strand, within Dsui_0818 at 830.111 kb on + strand, within Dsui_0818 at 830.111 kb on + strand, within Dsui_0818 at 830.147 kb on + strand, within Dsui_0818 at 830.147 kb on + strand, within Dsui_0818 at 830.155 kb on - strand, within Dsui_0818 at 830.175 kb on + strand, within Dsui_0818 at 830.175 kb on + strand, within Dsui_0818 at 830.175 kb on - strand, within Dsui_0818 at 830.176 kb on + strand, within Dsui_0818 at 830.203 kb on + strand, within Dsui_0818 at 830.203 kb on + strand, within Dsui_0818 at 830.213 kb on + strand, within Dsui_0818 at 830.221 kb on - strand, within Dsui_0818 at 830.234 kb on - strand at 830.286 kb on - strand at 830.298 kb on + strand at 830.309 kb on + strand at 830.364 kb on + strand at 830.376 kb on - strand at 830.453 kb on + strand at 830.461 kb on - strand, within Dsui_0819 at 830.500 kb on + strand, within Dsui_0819 at 830.543 kb on - strand, within Dsui_0819 at 830.546 kb on + strand, within Dsui_0819 at 830.547 kb on + strand, within Dsui_0819 at 830.555 kb on - strand, within Dsui_0819 at 830.555 kb on - strand, within Dsui_0819 at 830.555 kb on - strand, within Dsui_0819 at 830.581 kb on + strand, within Dsui_0819 at 830.595 kb on + strand, within Dsui_0819 at 830.603 kb on - strand, within Dsui_0819 at 830.605 kb on + strand, within Dsui_0819 at 830.634 kb on - strand, within Dsui_0819 at 830.645 kb on + strand, within Dsui_0819 at 830.653 kb on - strand, within Dsui_0819 at 830.658 kb on - strand, within Dsui_0819 at 830.662 kb on + strand, within Dsui_0819 at 830.662 kb on + strand, within Dsui_0819 at 830.685 kb on + strand, within Dsui_0819 at 830.688 kb on - strand, within Dsui_0819 at 830.688 kb on - strand, within Dsui_0819 at 830.693 kb on - strand, within Dsui_0819 at 830.693 kb on - strand, within Dsui_0819 at 830.693 kb on - strand, within Dsui_0819 at 830.693 kb on - strand, within Dsui_0819 at 830.718 kb on + strand, within Dsui_0819 at 830.726 kb on - strand, within Dsui_0819 at 830.733 kb on - strand, within Dsui_0819 at 830.733 kb on - strand, within Dsui_0819 at 830.771 kb on - strand, within Dsui_0819 at 830.801 kb on - strand, within Dsui_0819 at 830.817 kb on - strand, within Dsui_0819 at 830.858 kb on + strand, within Dsui_0819 at 830.879 kb on + strand, within Dsui_0819 at 830.879 kb on + strand, within Dsui_0819 at 830.887 kb on - strand, within Dsui_0819 at 830.929 kb on + strand, within Dsui_0819 at 830.962 kb on - strand, within Dsui_0819 at 831.017 kb on + strand, within Dsui_0819 at 831.019 kb on - strand, within Dsui_0819 at 831.020 kb on - strand, within Dsui_0819 at 831.020 kb on - strand, within Dsui_0819 at 831.025 kb on - strand, within Dsui_0819 at 831.029 kb on + strand, within Dsui_0819 at 831.088 kb on + strand, within Dsui_0819 at 831.112 kb on + strand, within Dsui_0819 at 831.171 kb on - strand, within Dsui_0819 at 831.183 kb on + strand, within Dsui_0819 at 831.330 kb on - strand, within Dsui_0819 at 831.330 kb on - strand, within Dsui_0819 at 831.330 kb on - strand, within Dsui_0819 at 831.332 kb on + strand, within Dsui_0819
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 828,639 + +0.7 828,639 + +0.2 828,639 + +0.4 828,639 - +1.0 828,639 - +0.5 828,639 - -0.1 828,644 + +1.4 828,644 - +1.0 828,647 - +1.1 828,647 - +0.8 828,652 - -0.1 829,151 - -0.1 829,155 + -4.6 829,163 - -3.1 829,189 + -0.1 829,225 + +0.7 829,225 + +0.9 829,232 + +0.7 829,232 + +0.4 829,232 + +1.5 829,232 + +1.2 829,232 + -0.7 829,232 + +0.6 829,232 + +0.4 829,232 + +0.5 829,235 - +0.8 829,240 - +1.0 829,240 - -0.2 829,240 - +1.7 829,240 - +0.7 829,240 - +0.6 829,266 - +0.0 829,266 - -1.5 829,266 - +0.0 829,311 + +0.7 829,311 + +1.0 829,311 + +0.4 829,311 + +0.5 829,311 + +0.5 829,311 + +0.6 829,319 - +0.5 829,319 - +0.3 829,319 - -0.1 829,319 - +0.5 829,343 + +0.4 829,382 - -2.4 829,404 - Dsui_0818 0.11 -1.7 829,438 - Dsui_0818 0.15 -2.6 829,473 + Dsui_0818 0.18 +0.6 829,473 + Dsui_0818 0.18 +1.1 829,481 - Dsui_0818 0.19 -1.0 829,481 - Dsui_0818 0.19 +0.0 829,574 + Dsui_0818 0.27 +0.6 829,620 + Dsui_0818 0.32 +0.9 829,628 + Dsui_0818 0.33 -0.4 829,628 - Dsui_0818 0.33 -0.1 829,677 - Dsui_0818 0.37 -0.7 829,681 - Dsui_0818 0.38 -0.2 829,696 - Dsui_0818 0.39 -0.0 829,702 - Dsui_0818 0.40 +0.4 829,709 + Dsui_0818 0.40 +1.3 829,768 - Dsui_0818 0.46 +0.2 829,792 - Dsui_0818 0.48 +1.8 829,792 - Dsui_0818 0.48 -0.4 829,792 - Dsui_0818 0.48 +0.1 829,792 - Dsui_0818 0.48 -1.3 829,806 - Dsui_0818 0.49 +1.1 829,806 - Dsui_0818 0.49 -0.9 829,852 - Dsui_0818 0.54 +0.0 829,852 - Dsui_0818 0.54 +0.8 829,863 - Dsui_0818 0.55 +0.6 829,864 + Dsui_0818 0.55 +0.4 829,880 - Dsui_0818 0.56 +0.2 829,880 - Dsui_0818 0.56 -1.0 829,887 + Dsui_0818 0.57 +0.9 829,911 - Dsui_0818 0.59 +0.5 829,911 - Dsui_0818 0.59 +0.7 830,014 + Dsui_0818 0.69 +0.4 830,015 - Dsui_0818 0.69 -0.2 830,017 - Dsui_0818 0.69 +1.4 830,043 + Dsui_0818 0.72 +1.9 830,050 - Dsui_0818 0.73 -0.4 830,064 + Dsui_0818 0.74 +0.9 830,064 + Dsui_0818 0.74 +0.4 830,072 - Dsui_0818 0.75 +0.9 830,103 - Dsui_0818 0.78 +0.3 830,103 - Dsui_0818 0.78 +1.2 830,103 - Dsui_0818 0.78 +0.2 830,111 + Dsui_0818 0.78 +0.4 830,111 + Dsui_0818 0.78 -0.0 830,111 + Dsui_0818 0.78 +0.8 830,147 + Dsui_0818 0.82 -1.3 830,147 + Dsui_0818 0.82 +0.8 830,155 - Dsui_0818 0.82 -0.2 830,175 + Dsui_0818 0.84 +0.7 830,175 + Dsui_0818 0.84 +0.3 830,175 - Dsui_0818 0.84 -1.6 830,176 + Dsui_0818 0.84 +0.5 830,203 + Dsui_0818 0.87 -1.1 830,203 + Dsui_0818 0.87 +0.6 830,213 + Dsui_0818 0.88 +1.1 830,221 - Dsui_0818 0.89 +0.0 830,234 - -0.4 830,286 - -0.6 830,298 + +0.9 830,309 + -0.3 830,364 + +0.9 830,376 - -1.9 830,453 + +1.1 830,461 - Dsui_0819 0.10 +0.1 830,500 + Dsui_0819 0.13 -0.3 830,543 - Dsui_0819 0.17 +0.2 830,546 + Dsui_0819 0.18 +1.2 830,547 + Dsui_0819 0.18 +1.2 830,555 - Dsui_0819 0.18 -0.5 830,555 - Dsui_0819 0.18 -0.0 830,555 - Dsui_0819 0.18 +0.8 830,581 + Dsui_0819 0.21 +1.5 830,595 + Dsui_0819 0.22 -0.1 830,603 - Dsui_0819 0.23 +0.7 830,605 + Dsui_0819 0.23 +1.2 830,634 - Dsui_0819 0.25 +0.3 830,645 + Dsui_0819 0.26 +0.2 830,653 - Dsui_0819 0.27 +0.7 830,658 - Dsui_0819 0.28 +0.5 830,662 + Dsui_0819 0.28 -0.4 830,662 + Dsui_0819 0.28 +0.4 830,685 + Dsui_0819 0.30 +0.3 830,688 - Dsui_0819 0.30 +0.2 830,688 - Dsui_0819 0.30 +0.5 830,693 - Dsui_0819 0.31 -0.2 830,693 - Dsui_0819 0.31 +0.2 830,693 - Dsui_0819 0.31 +0.1 830,693 - Dsui_0819 0.31 +0.3 830,718 + Dsui_0819 0.33 +0.5 830,726 - Dsui_0819 0.34 +0.1 830,733 - Dsui_0819 0.34 +0.3 830,733 - Dsui_0819 0.34 +0.3 830,771 - Dsui_0819 0.38 +0.3 830,801 - Dsui_0819 0.40 +0.6 830,817 - Dsui_0819 0.42 +0.3 830,858 + Dsui_0819 0.45 -0.1 830,879 + Dsui_0819 0.47 -0.0 830,879 + Dsui_0819 0.47 -0.0 830,887 - Dsui_0819 0.48 -0.2 830,929 + Dsui_0819 0.52 +1.0 830,962 - Dsui_0819 0.55 -0.1 831,017 + Dsui_0819 0.60 -0.1 831,019 - Dsui_0819 0.60 +0.5 831,020 - Dsui_0819 0.60 -2.0 831,020 - Dsui_0819 0.60 +0.9 831,025 - Dsui_0819 0.60 +1.2 831,029 + Dsui_0819 0.61 +0.6 831,088 + Dsui_0819 0.66 -0.1 831,112 + Dsui_0819 0.68 +0.3 831,171 - Dsui_0819 0.73 -0.6 831,183 + Dsui_0819 0.75 +0.2 831,330 - Dsui_0819 0.88 -2.0 831,330 - Dsui_0819 0.88 +0.3 831,330 - Dsui_0819 0.88 +0.1 831,332 + Dsui_0819 0.88 +0.5
Or see this region's nucleotide sequence