Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0742 and Dsui_0743 are separated by 88 nucleotides Dsui_0743 and Dsui_0744 are separated by 29 nucleotides Dsui_0744 and Dsui_0745 are separated by 199 nucleotides Dsui_0745 and Dsui_0746 overlap by 13 nucleotides
Dsui_0742: Dsui_0742 - phosphoenolpyruvate-protein phosphotransferase, at 740,052 to 741,791
_0742
Dsui_0743: Dsui_0743 - phosphotransferase system HPr (HPr) family protein, at 741,880 to 742,158
_0743
Dsui_0744: Dsui_0744 - phosphotransferase system, mannose/fructose-specific component IIA, at 742,188 to 742,580
_0744
Dsui_0745: Dsui_0745 - membrane-associated lipoprotein involved in thiamine biosynthesis, at 742,780 to 743,820
_0745
Dsui_0746: Dsui_0746 - glutathione synthetase, at 743,808 to 744,755
_0746
Position (kb) 742
743
744 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 741.798 kb on + strand at 741.806 kb on + strand at 741.921 kb on - strand, within Dsui_0743 at 741.921 kb on - strand, within Dsui_0743 at 741.957 kb on + strand, within Dsui_0743 at 741.965 kb on - strand, within Dsui_0743 at 742.049 kb on - strand, within Dsui_0743 at 742.147 kb on + strand at 742.152 kb on + strand at 742.193 kb on - strand at 742.194 kb on - strand at 742.199 kb on - strand at 742.236 kb on - strand, within Dsui_0744 at 742.295 kb on - strand, within Dsui_0744 at 742.324 kb on + strand, within Dsui_0744 at 742.511 kb on - strand, within Dsui_0744 at 742.530 kb on - strand, within Dsui_0744 at 742.532 kb on + strand, within Dsui_0744 at 742.584 kb on + strand at 742.644 kb on + strand at 742.668 kb on + strand at 742.771 kb on - strand at 742.811 kb on - strand at 742.811 kb on - strand at 742.954 kb on - strand, within Dsui_0745 at 742.959 kb on - strand, within Dsui_0745 at 743.025 kb on - strand, within Dsui_0745 at 743.076 kb on + strand at 743.138 kb on - strand, within Dsui_0745 at 743.281 kb on + strand, within Dsui_0745 at 743.771 kb on + strand at 743.863 kb on - strand at 743.955 kb on - strand, within Dsui_0746 at 744.017 kb on + strand, within Dsui_0746 at 744.104 kb on - strand, within Dsui_0746 at 744.137 kb on - strand, within Dsui_0746 at 744.152 kb on - strand, within Dsui_0746 at 744.165 kb on + strand, within Dsui_0746 at 744.192 kb on + strand, within Dsui_0746 at 744.245 kb on + strand at 744.325 kb on - strand, within Dsui_0746 at 744.350 kb on - strand, within Dsui_0746 at 744.358 kb on - strand, within Dsui_0746 at 744.403 kb on + strand, within Dsui_0746 at 744.436 kb on + strand, within Dsui_0746 at 744.438 kb on - strand, within Dsui_0746 at 744.455 kb on + strand at 744.459 kb on + strand at 744.471 kb on - strand, within Dsui_0746 at 744.545 kb on - strand, within Dsui_0746 at 744.626 kb on + strand, within Dsui_0746 at 744.626 kb on + strand, within Dsui_0746 at 744.654 kb on + strand, within Dsui_0746 at 744.712 kb on + strand at 744.712 kb on + strand at 744.712 kb on + strand at 744.717 kb on + strand at 744.720 kb on - strand at 744.722 kb on + strand at 744.722 kb on + strand at 744.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 741,798 + -1.9 741,806 + +0.0 741,921 - Dsui_0743 0.15 +0.2 741,921 - Dsui_0743 0.15 -0.5 741,957 + Dsui_0743 0.28 -0.2 741,965 - Dsui_0743 0.30 +0.4 742,049 - Dsui_0743 0.61 -0.0 742,147 + -0.5 742,152 + +0.0 742,193 - -0.3 742,194 - +0.6 742,199 - -0.7 742,236 - Dsui_0744 0.12 -3.5 742,295 - Dsui_0744 0.27 -1.9 742,324 + Dsui_0744 0.35 -5.6 742,511 - Dsui_0744 0.82 -1.0 742,530 - Dsui_0744 0.87 -3.9 742,532 + Dsui_0744 0.88 -2.3 742,584 + -2.9 742,644 + +1.3 742,668 + -1.4 742,771 - +0.5 742,811 - +0.5 742,811 - +0.2 742,954 - Dsui_0745 0.17 -1.8 742,959 - Dsui_0745 0.17 +2.0 743,025 - Dsui_0745 0.24 -1.0 743,076 + -3.5 743,138 - Dsui_0745 0.34 -2.0 743,281 + Dsui_0745 0.48 -1.6 743,771 + -2.7 743,863 - +0.2 743,955 - Dsui_0746 0.16 -2.6 744,017 + Dsui_0746 0.22 -0.8 744,104 - Dsui_0746 0.31 +0.5 744,137 - Dsui_0746 0.35 +0.7 744,152 - Dsui_0746 0.36 -0.5 744,165 + Dsui_0746 0.38 -0.2 744,192 + Dsui_0746 0.41 -0.5 744,245 + -1.9 744,325 - Dsui_0746 0.55 +0.6 744,350 - Dsui_0746 0.57 +1.1 744,358 - Dsui_0746 0.58 +0.7 744,403 + Dsui_0746 0.63 -0.9 744,436 + Dsui_0746 0.66 +0.2 744,438 - Dsui_0746 0.66 -0.1 744,455 + +1.4 744,459 + -0.5 744,471 - Dsui_0746 0.70 +0.5 744,545 - Dsui_0746 0.78 +0.2 744,626 + Dsui_0746 0.86 -1.2 744,626 + Dsui_0746 0.86 -1.4 744,654 + Dsui_0746 0.89 +0.3 744,712 + -0.8 744,712 + -0.5 744,712 + +1.2 744,717 + -0.9 744,720 - -0.8 744,722 + +1.1 744,722 + -0.4 744,803 + +0.6
Or see this region's nucleotide sequence