Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0541 and Dsui_0542 are separated by 117 nucleotides Dsui_0542 and Dsui_0543 are separated by 228 nucleotides
Dsui_0541: Dsui_0541 - cobalamin biosynthesis protein CobD, at 545,123 to 546,094
_0541
Dsui_0542: Dsui_0542 - cobyric acid synthase CobQ, at 546,212 to 547,705
_0542
Dsui_0543: Dsui_0543 - malic enzyme, at 547,934 to 550,210
_0543
Position (kb) 546
547
548 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 545.537 kb on - strand, within Dsui_0541 at 545.556 kb on + strand, within Dsui_0541 at 545.564 kb on - strand, within Dsui_0541 at 545.612 kb on - strand, within Dsui_0541 at 545.612 kb on - strand, within Dsui_0541 at 545.650 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.682 kb on - strand, within Dsui_0541 at 545.682 kb on - strand, within Dsui_0541 at 545.697 kb on - strand, within Dsui_0541 at 545.733 kb on + strand at 545.738 kb on + strand, within Dsui_0541 at 545.799 kb on - strand, within Dsui_0541 at 545.861 kb on + strand at 545.886 kb on - strand, within Dsui_0541 at 545.922 kb on - strand, within Dsui_0541 at 546.037 kb on - strand at 546.076 kb on + strand at 546.079 kb on - strand at 546.084 kb on - strand at 546.084 kb on - strand at 546.175 kb on + strand at 546.225 kb on + strand at 546.225 kb on + strand at 546.233 kb on + strand at 546.233 kb on + strand at 546.238 kb on + strand at 546.241 kb on - strand at 546.241 kb on - strand at 546.241 kb on - strand at 546.241 kb on - strand at 546.241 kb on - strand at 546.385 kb on + strand at 546.393 kb on - strand, within Dsui_0542 at 546.747 kb on + strand, within Dsui_0542 at 546.755 kb on - strand, within Dsui_0542 at 546.757 kb on + strand, within Dsui_0542 at 546.783 kb on + strand, within Dsui_0542 at 546.846 kb on + strand, within Dsui_0542 at 546.900 kb on + strand, within Dsui_0542 at 546.900 kb on + strand, within Dsui_0542 at 546.950 kb on + strand, within Dsui_0542 at 546.970 kb on + strand, within Dsui_0542 at 547.002 kb on + strand at 547.049 kb on + strand, within Dsui_0542 at 547.131 kb on - strand, within Dsui_0542 at 547.262 kb on + strand, within Dsui_0542 at 547.374 kb on - strand, within Dsui_0542 at 547.424 kb on + strand, within Dsui_0542 at 547.432 kb on - strand, within Dsui_0542 at 547.622 kb on + strand at 547.918 kb on + strand at 547.918 kb on + strand at 547.924 kb on + strand at 547.925 kb on + strand at 547.926 kb on - strand at 548.015 kb on + strand at 548.071 kb on - strand at 548.092 kb on + strand at 548.098 kb on - strand at 548.100 kb on - strand at 548.100 kb on - strand at 548.172 kb on - strand, within Dsui_0543 at 548.307 kb on + strand, within Dsui_0543 at 548.482 kb on + strand, within Dsui_0543 at 548.593 kb on + strand, within Dsui_0543 at 548.613 kb on - strand, within Dsui_0543 at 548.613 kb on - strand at 548.688 kb on - strand, within Dsui_0543
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 545,537 - Dsui_0541 0.43 -1.2 545,556 + Dsui_0541 0.45 -0.3 545,564 - Dsui_0541 0.45 -0.4 545,612 - Dsui_0541 0.50 +0.6 545,612 - Dsui_0541 0.50 -1.3 545,650 - Dsui_0541 0.54 -1.5 545,671 - Dsui_0541 0.56 -0.9 545,671 - Dsui_0541 0.56 -0.7 545,671 - Dsui_0541 0.56 +0.8 545,682 - Dsui_0541 0.58 -1.0 545,682 - Dsui_0541 0.58 -1.4 545,697 - Dsui_0541 0.59 -0.3 545,733 + -0.4 545,738 + Dsui_0541 0.63 +0.0 545,799 - Dsui_0541 0.70 -0.3 545,861 + -0.2 545,886 - Dsui_0541 0.78 +0.0 545,922 - Dsui_0541 0.82 +0.4 546,037 - -0.1 546,076 + +0.3 546,079 - +0.9 546,084 - +0.5 546,084 - +1.8 546,175 + +1.5 546,225 + +0.2 546,225 + -0.7 546,233 + +0.3 546,233 + -1.6 546,238 + -0.2 546,241 - +0.0 546,241 - +1.6 546,241 - -0.8 546,241 - -0.2 546,241 - +0.7 546,385 + -2.7 546,393 - Dsui_0542 0.12 -0.1 546,747 + Dsui_0542 0.36 -0.2 546,755 - Dsui_0542 0.36 -0.9 546,757 + Dsui_0542 0.36 -0.3 546,783 + Dsui_0542 0.38 -0.4 546,846 + Dsui_0542 0.42 -0.1 546,900 + Dsui_0542 0.46 -0.6 546,900 + Dsui_0542 0.46 -0.7 546,950 + Dsui_0542 0.49 -0.6 546,970 + Dsui_0542 0.51 +0.4 547,002 + -0.2 547,049 + Dsui_0542 0.56 -0.6 547,131 - Dsui_0542 0.62 -0.3 547,262 + Dsui_0542 0.70 +0.3 547,374 - Dsui_0542 0.78 -0.2 547,424 + Dsui_0542 0.81 -0.7 547,432 - Dsui_0542 0.82 -0.3 547,622 + -1.3 547,918 + -2.5 547,918 + -0.0 547,924 + +0.2 547,925 + -3.0 547,926 - +0.2 548,015 + +0.9 548,071 - -0.2 548,092 + +1.7 548,098 - -3.2 548,100 - +1.3 548,100 - -0.2 548,172 - Dsui_0543 0.10 -0.9 548,307 + Dsui_0543 0.16 +0.5 548,482 + Dsui_0543 0.24 +1.4 548,593 + Dsui_0543 0.29 +0.5 548,613 - Dsui_0543 0.30 -0.6 548,613 - -2.2 548,688 - Dsui_0543 0.33 -1.1
Or see this region's nucleotide sequence