Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0303 and Dsui_0304 are separated by 36 nucleotides Dsui_0304 and Dsui_0305 are separated by 53 nucleotides Dsui_0305 and Dsui_0306 are separated by 136 nucleotides Dsui_0306 and Dsui_0307 are separated by 31 nucleotides
Dsui_0303: Dsui_0303 - 4-hydroxybenzoate polyprenyl transferase, proteobacterial, at 304,986 to 305,897
_0303
Dsui_0304: Dsui_0304 - hypothetical protein, at 305,934 to 306,182
_0304
Dsui_0305: Dsui_0305 - transcriptional regulator, at 306,236 to 307,129
_0305
Dsui_0306: Dsui_0306 - putative permease, at 307,266 to 308,054
_0306
Dsui_0307: Dsui_0307 - methyl-accepting chemotaxis protein, at 308,086 to 309,693
_0307
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 305.992 kb on - strand, within Dsui_0304 at 306.044 kb on + strand at 306.100 kb on - strand, within Dsui_0304 at 306.103 kb on + strand at 306.107 kb on + strand, within Dsui_0304 at 306.185 kb on - strand at 306.259 kb on - strand at 306.286 kb on - strand at 306.323 kb on - strand at 306.333 kb on + strand, within Dsui_0305 at 306.424 kb on + strand, within Dsui_0305 at 306.481 kb on + strand at 306.665 kb on - strand, within Dsui_0305 at 306.692 kb on + strand at 306.715 kb on - strand, within Dsui_0305 at 306.733 kb on + strand, within Dsui_0305 at 306.770 kb on + strand at 306.918 kb on + strand at 306.988 kb on + strand, within Dsui_0305 at 307.077 kb on - strand at 307.109 kb on + strand at 307.369 kb on - strand, within Dsui_0306 at 307.597 kb on - strand, within Dsui_0306 at 307.665 kb on + strand, within Dsui_0306 at 307.714 kb on - strand at 307.803 kb on - strand, within Dsui_0306 at 307.848 kb on - strand, within Dsui_0306 at 307.936 kb on + strand, within Dsui_0306 at 308.070 kb on + strand at 308.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 305,992 - Dsui_0304 0.23 -2.1 306,044 + -1.5 306,100 - Dsui_0304 0.67 +0.4 306,103 + +0.7 306,107 + Dsui_0304 0.69 -0.8 306,185 - +0.1 306,259 - +0.7 306,286 - +0.1 306,323 - +1.0 306,333 + Dsui_0305 0.11 -0.7 306,424 + Dsui_0305 0.21 -0.5 306,481 + +0.3 306,665 - Dsui_0305 0.48 +0.8 306,692 + -2.9 306,715 - Dsui_0305 0.54 -0.5 306,733 + Dsui_0305 0.56 -1.3 306,770 + -2.3 306,918 + +0.5 306,988 + Dsui_0305 0.84 -1.8 307,077 - -0.1 307,109 + -2.8 307,369 - Dsui_0306 0.13 -0.7 307,597 - Dsui_0306 0.42 -0.1 307,665 + Dsui_0306 0.51 -1.4 307,714 - +2.0 307,803 - Dsui_0306 0.68 -0.8 307,848 - Dsui_0306 0.74 +0.7 307,936 + Dsui_0306 0.85 -1.2 308,070 + -0.5 308,120 - +0.1
Or see this region's nucleotide sequence