Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0300 and Dsui_0301 are separated by 31 nucleotides Dsui_0301 and Dsui_0302 are separated by 160 nucleotides
Dsui_0300: Dsui_0300 - response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain, at 300,996 to 302,621
_0300
Dsui_0301: Dsui_0301 - histidine kinase, at 302,653 to 303,342
_0301
Dsui_0302: Dsui_0302 - UbiD family decarboxylase, at 303,503 to 304,978
_0302
Position (kb) 302
303
304 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 301.698 kb on + strand, within Dsui_0300 at 301.706 kb on - strand, within Dsui_0300 at 301.787 kb on - strand, within Dsui_0300 at 301.896 kb on + strand, within Dsui_0300 at 301.903 kb on + strand, within Dsui_0300 at 301.923 kb on - strand, within Dsui_0300 at 301.935 kb on + strand, within Dsui_0300 at 301.956 kb on - strand, within Dsui_0300 at 301.987 kb on - strand, within Dsui_0300 at 302.002 kb on - strand, within Dsui_0300 at 302.079 kb on + strand, within Dsui_0300 at 302.124 kb on + strand, within Dsui_0300 at 302.127 kb on - strand, within Dsui_0300 at 302.169 kb on + strand, within Dsui_0300 at 302.221 kb on - strand, within Dsui_0300 at 302.271 kb on - strand, within Dsui_0300 at 302.271 kb on - strand, within Dsui_0300 at 302.297 kb on - strand, within Dsui_0300 at 302.307 kb on - strand, within Dsui_0300 at 302.512 kb on - strand at 302.516 kb on + strand at 302.524 kb on - strand at 302.538 kb on + strand at 302.649 kb on - strand at 302.651 kb on + strand at 302.662 kb on + strand at 302.882 kb on - strand, within Dsui_0301 at 302.924 kb on + strand at 302.987 kb on + strand, within Dsui_0301 at 303.007 kb on + strand, within Dsui_0301 at 303.061 kb on - strand, within Dsui_0301 at 303.315 kb on + strand at 303.323 kb on - strand at 303.433 kb on - strand at 303.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 301,698 + Dsui_0300 0.43 +0.6 301,706 - Dsui_0300 0.44 +1.0 301,787 - Dsui_0300 0.49 +0.3 301,896 + Dsui_0300 0.55 -1.2 301,903 + Dsui_0300 0.56 +0.4 301,923 - Dsui_0300 0.57 +0.9 301,935 + Dsui_0300 0.58 -0.2 301,956 - Dsui_0300 0.59 +0.8 301,987 - Dsui_0300 0.61 +0.5 302,002 - Dsui_0300 0.62 +0.9 302,079 + Dsui_0300 0.67 -0.4 302,124 + Dsui_0300 0.69 +1.1 302,127 - Dsui_0300 0.70 -0.2 302,169 + Dsui_0300 0.72 +0.2 302,221 - Dsui_0300 0.75 +0.1 302,271 - Dsui_0300 0.78 +0.4 302,271 - Dsui_0300 0.78 +0.5 302,297 - Dsui_0300 0.80 +2.6 302,307 - Dsui_0300 0.81 +0.7 302,512 - +0.2 302,516 + +3.2 302,524 - -1.8 302,538 + -0.4 302,649 - +0.0 302,651 + +0.3 302,662 + -0.1 302,882 - Dsui_0301 0.33 +0.4 302,924 + +2.0 302,987 + Dsui_0301 0.48 -0.9 303,007 + Dsui_0301 0.51 +0.8 303,061 - Dsui_0301 0.59 +2.0 303,315 + +1.8 303,323 - +1.1 303,433 - +1.1 303,516 - +1.1
Or see this region's nucleotide sequence