Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0130 and Dsui_0131 are separated by 445 nucleotides Dsui_0131 and Dsui_0132 are separated by 101 nucleotides
Dsui_0130: Dsui_0130 - malic enzyme, at 128,463 to 130,739
_0130
Dsui_0131: Dsui_0131 - phosphoenolpyruvate carboxykinase (GTP), at 131,185 to 133,044
_0131
Dsui_0132: Dsui_0132 - aspartate/tyrosine/aromatic aminotransferase, at 133,146 to 134,303
_0132
Position (kb) 131
132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 130.308 kb on - strand, within Dsui_0130 at 130.311 kb on + strand, within Dsui_0130 at 130.318 kb on + strand, within Dsui_0130 at 130.326 kb on - strand, within Dsui_0130 at 130.326 kb on - strand, within Dsui_0130 at 130.342 kb on - strand, within Dsui_0130 at 130.363 kb on - strand, within Dsui_0130 at 130.380 kb on - strand, within Dsui_0130 at 130.438 kb on + strand at 130.460 kb on - strand, within Dsui_0130 at 130.461 kb on - strand, within Dsui_0130 at 130.631 kb on - strand at 130.660 kb on - strand at 130.660 kb on - strand at 130.677 kb on - strand at 130.699 kb on + strand at 130.714 kb on + strand at 130.722 kb on - strand at 130.732 kb on - strand at 130.741 kb on + strand at 130.781 kb on + strand at 130.791 kb on + strand at 130.799 kb on - strand at 130.799 kb on - strand at 130.815 kb on + strand at 130.823 kb on - strand at 130.823 kb on - strand at 130.823 kb on - strand at 130.823 kb on - strand at 130.829 kb on - strand at 130.834 kb on + strand at 130.842 kb on - strand at 130.878 kb on + strand at 130.879 kb on + strand at 130.886 kb on - strand at 130.887 kb on - strand at 130.955 kb on + strand at 131.012 kb on - strand at 131.037 kb on - strand at 131.054 kb on + strand at 131.057 kb on - strand at 131.062 kb on - strand at 131.089 kb on + strand at 131.093 kb on + strand at 131.099 kb on + strand at 131.099 kb on + strand at 131.122 kb on + strand at 131.172 kb on - strand at 131.175 kb on + strand at 131.175 kb on + strand at 131.175 kb on + strand at 131.175 kb on + strand at 131.183 kb on - strand at 131.183 kb on - strand at 131.183 kb on - strand at 131.199 kb on - strand at 131.201 kb on + strand at 131.201 kb on + strand at 131.211 kb on + strand at 131.211 kb on + strand at 131.229 kb on + strand at 131.236 kb on + strand at 131.247 kb on + strand at 131.273 kb on + strand at 131.277 kb on + strand at 131.277 kb on + strand at 131.277 kb on + strand at 131.277 kb on + strand at 131.277 kb on + strand at 131.277 kb on + strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.285 kb on - strand at 131.291 kb on + strand at 131.309 kb on + strand at 131.309 kb on + strand at 131.339 kb on - strand at 131.364 kb on + strand at 131.372 kb on - strand, within Dsui_0131 at 131.396 kb on - strand at 131.408 kb on + strand, within Dsui_0131 at 131.408 kb on + strand, within Dsui_0131 at 131.416 kb on - strand, within Dsui_0131 at 131.417 kb on + strand, within Dsui_0131 at 131.422 kb on + strand, within Dsui_0131 at 131.434 kb on + strand, within Dsui_0131 at 131.456 kb on + strand, within Dsui_0131 at 131.482 kb on - strand, within Dsui_0131 at 131.493 kb on - strand at 131.506 kb on + strand, within Dsui_0131 at 131.535 kb on + strand, within Dsui_0131 at 131.535 kb on + strand, within Dsui_0131 at 131.535 kb on + strand, within Dsui_0131 at 131.535 kb on + strand, within Dsui_0131 at 131.543 kb on - strand, within Dsui_0131 at 131.543 kb on - strand, within Dsui_0131 at 131.576 kb on - strand, within Dsui_0131 at 131.663 kb on + strand, within Dsui_0131 at 131.676 kb on + strand, within Dsui_0131 at 131.703 kb on - strand, within Dsui_0131 at 131.705 kb on + strand, within Dsui_0131 at 131.733 kb on + strand, within Dsui_0131 at 131.794 kb on + strand, within Dsui_0131 at 131.816 kb on - strand, within Dsui_0131 at 131.855 kb on - strand, within Dsui_0131 at 131.880 kb on + strand, within Dsui_0131 at 131.882 kb on + strand, within Dsui_0131 at 131.882 kb on + strand, within Dsui_0131 at 131.888 kb on - strand, within Dsui_0131 at 131.907 kb on - strand, within Dsui_0131 at 131.993 kb on + strand, within Dsui_0131 at 132.067 kb on - strand, within Dsui_0131 at 132.069 kb on + strand, within Dsui_0131 at 132.132 kb on + strand, within Dsui_0131 at 132.140 kb on - strand, within Dsui_0131 at 132.247 kb on + strand, within Dsui_0131 at 132.247 kb on + strand, within Dsui_0131 at 132.255 kb on - strand, within Dsui_0131 at 132.275 kb on + strand, within Dsui_0131 at 132.278 kb on - strand, within Dsui_0131 at 132.283 kb on - strand, within Dsui_0131 at 132.335 kb on - strand, within Dsui_0131 at 132.353 kb on - strand, within Dsui_0131 at 132.485 kb on + strand, within Dsui_0131 at 132.526 kb on + strand, within Dsui_0131 at 132.532 kb on - strand, within Dsui_0131 at 132.539 kb on + strand, within Dsui_0131 at 132.839 kb on - strand, within Dsui_0131 at 133.024 kb on - strand at 133.026 kb on + strand at 133.026 kb on + strand at 133.034 kb on - strand at 133.107 kb on + strand at 133.114 kb on + strand at 133.122 kb on - strand at 133.122 kb on - strand at 133.200 kb on - strand at 133.257 kb on - strand at 133.339 kb on + strand, within Dsui_0132 at 133.339 kb on + strand, within Dsui_0132 at 133.344 kb on + strand, within Dsui_0132 at 133.352 kb on - strand, within Dsui_0132 at 133.353 kb on - strand, within Dsui_0132 at 133.467 kb on - strand, within Dsui_0132 at 133.472 kb on - strand, within Dsui_0132 at 133.554 kb on - strand at 133.622 kb on + strand, within Dsui_0132 at 133.622 kb on + strand, within Dsui_0132 at 133.649 kb on + strand, within Dsui_0132 at 133.650 kb on + strand, within Dsui_0132 at 133.684 kb on + strand, within Dsui_0132 at 133.685 kb on + strand, within Dsui_0132 at 133.822 kb on - strand, within Dsui_0132 at 133.835 kb on - strand, within Dsui_0132 at 133.840 kb on - strand, within Dsui_0132 at 133.885 kb on + strand at 133.895 kb on - strand, within Dsui_0132 at 133.895 kb on - strand, within Dsui_0132 at 133.961 kb on - strand, within Dsui_0132 at 133.998 kb on - strand, within Dsui_0132
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 130,308 - Dsui_0130 0.81 +1.5 130,311 + Dsui_0130 0.81 -3.6 130,318 + Dsui_0130 0.81 +0.8 130,326 - Dsui_0130 0.82 +1.5 130,326 - Dsui_0130 0.82 +1.7 130,342 - Dsui_0130 0.83 +0.6 130,363 - Dsui_0130 0.83 +0.8 130,380 - Dsui_0130 0.84 +2.2 130,438 + -2.4 130,460 - Dsui_0130 0.88 +0.2 130,461 - Dsui_0130 0.88 +0.3 130,631 - +0.6 130,660 - +1.6 130,660 - +0.6 130,677 - +1.6 130,699 + -0.7 130,714 + -2.8 130,722 - +1.1 130,732 - +1.1 130,741 + -1.0 130,781 + -3.3 130,791 + -0.3 130,799 - +1.3 130,799 - +3.7 130,815 + +1.4 130,823 - +1.1 130,823 - +1.7 130,823 - -0.9 130,823 - -0.5 130,829 - +0.6 130,834 + +0.9 130,842 - +0.7 130,878 + +1.3 130,879 + +1.6 130,886 - +0.6 130,887 - +0.6 130,955 + +1.2 131,012 - -0.7 131,037 - +0.0 131,054 + +1.0 131,057 - +1.1 131,062 - +0.6 131,089 + +0.5 131,093 + +0.3 131,099 + +1.3 131,099 + -0.6 131,122 + +0.0 131,172 - -1.3 131,175 + +1.6 131,175 + +1.6 131,175 + +1.1 131,175 + +1.4 131,183 - -0.6 131,183 - -1.3 131,183 - +1.7 131,199 - +2.1 131,201 + +1.4 131,201 + +1.5 131,211 + +2.7 131,211 + +1.7 131,229 + +1.6 131,236 + +0.3 131,247 + +3.7 131,273 + +1.4 131,277 + +1.3 131,277 + +1.6 131,277 + +1.5 131,277 + +1.8 131,277 + +2.4 131,277 + +2.1 131,285 - +1.7 131,285 - +1.2 131,285 - +0.7 131,285 - +0.4 131,285 - +1.8 131,285 - -0.6 131,285 - +2.7 131,291 + +0.8 131,309 + +1.8 131,309 + +1.3 131,339 - -3.3 131,364 + +1.2 131,372 - Dsui_0131 0.10 -0.9 131,396 - -1.3 131,408 + Dsui_0131 0.12 +1.6 131,408 + Dsui_0131 0.12 +0.9 131,416 - Dsui_0131 0.12 -1.2 131,417 + Dsui_0131 0.12 +1.8 131,422 + Dsui_0131 0.13 +2.1 131,434 + Dsui_0131 0.13 +1.4 131,456 + Dsui_0131 0.15 +1.9 131,482 - Dsui_0131 0.16 +0.6 131,493 - -1.2 131,506 + Dsui_0131 0.17 +1.5 131,535 + Dsui_0131 0.19 +1.2 131,535 + Dsui_0131 0.19 +1.1 131,535 + Dsui_0131 0.19 +1.5 131,535 + Dsui_0131 0.19 +1.4 131,543 - Dsui_0131 0.19 +1.3 131,543 - Dsui_0131 0.19 -3.0 131,576 - Dsui_0131 0.21 -0.8 131,663 + Dsui_0131 0.26 +0.7 131,676 + Dsui_0131 0.26 +1.2 131,703 - Dsui_0131 0.28 -1.9 131,705 + Dsui_0131 0.28 +1.3 131,733 + Dsui_0131 0.29 +1.2 131,794 + Dsui_0131 0.33 +0.7 131,816 - Dsui_0131 0.34 +0.0 131,855 - Dsui_0131 0.36 +1.2 131,880 + Dsui_0131 0.37 +0.7 131,882 + Dsui_0131 0.37 +2.2 131,882 + Dsui_0131 0.37 +1.3 131,888 - Dsui_0131 0.38 +1.6 131,907 - Dsui_0131 0.39 +0.1 131,993 + Dsui_0131 0.43 +2.5 132,067 - Dsui_0131 0.47 +0.4 132,069 + Dsui_0131 0.48 +1.6 132,132 + Dsui_0131 0.51 +1.0 132,140 - Dsui_0131 0.51 +0.9 132,247 + Dsui_0131 0.57 +1.2 132,247 + Dsui_0131 0.57 +0.9 132,255 - Dsui_0131 0.58 +1.0 132,275 + Dsui_0131 0.59 +1.4 132,278 - Dsui_0131 0.59 +0.7 132,283 - Dsui_0131 0.59 +1.4 132,335 - Dsui_0131 0.62 -0.5 132,353 - Dsui_0131 0.63 +1.1 132,485 + Dsui_0131 0.70 +1.2 132,526 + Dsui_0131 0.72 +1.8 132,532 - Dsui_0131 0.72 +0.8 132,539 + Dsui_0131 0.73 +1.4 132,839 - Dsui_0131 0.89 +1.3 133,024 - +0.1 133,026 + +1.4 133,026 + +1.0 133,034 - +1.7 133,107 + +1.1 133,114 + +1.1 133,122 - -0.6 133,122 - +0.7 133,200 - -2.7 133,257 - +0.2 133,339 + Dsui_0132 0.17 +0.9 133,339 + Dsui_0132 0.17 -0.4 133,344 + Dsui_0132 0.17 +0.1 133,352 - Dsui_0132 0.18 -0.3 133,353 - Dsui_0132 0.18 +0.4 133,467 - Dsui_0132 0.28 +0.6 133,472 - Dsui_0132 0.28 -0.1 133,554 - +0.2 133,622 + Dsui_0132 0.41 +1.1 133,622 + Dsui_0132 0.41 +1.0 133,649 + Dsui_0132 0.43 -2.3 133,650 + Dsui_0132 0.44 -1.3 133,684 + Dsui_0132 0.46 -0.2 133,685 + Dsui_0132 0.47 +0.5 133,822 - Dsui_0132 0.58 +0.8 133,835 - Dsui_0132 0.59 +0.7 133,840 - Dsui_0132 0.60 +0.8 133,885 + +0.5 133,895 - Dsui_0132 0.65 +1.1 133,895 - Dsui_0132 0.65 +0.5 133,961 - Dsui_0132 0.70 +0.4 133,998 - Dsui_0132 0.74 +1.0
Or see this region's nucleotide sequence