Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_16625 and CSW01_16630 are separated by 114 nucleotides CSW01_16630 and CSW01_16635 are separated by 43 nucleotides CSW01_16635 and CSW01_16640 are separated by 62 nucleotides CSW01_16640 and CSW01_16645 overlap by 4 nucleotides CSW01_16645 and CSW01_16650 are separated by 69 nucleotides CSW01_16650 and CSW01_16655 are separated by 56 nucleotides
CSW01_16625: CSW01_16625 - acetyltransferase, at 419,349 to 419,471
_16625
CSW01_16630: CSW01_16630 - DUF645 domain-containing protein, at 419,586 to 419,766
_16630
CSW01_16635: CSW01_16635 - hypothetical protein, at 419,810 to 419,905
_16635
CSW01_16640: CSW01_16640 - N-acetyltransferase, at 419,968 to 420,465
_16640
CSW01_16645: CSW01_16645 - DUF1778 domain-containing protein, at 420,462 to 420,734
_16645
CSW01_16650: CSW01_16650 - hypothetical protein, at 420,804 to 420,908
_16650
CSW01_16655: CSW01_16655 - hypothetical protein, at 420,965 to 421,633
_16655
Position (kb) 420
421 Strain fitness (log2 ratio) -1
0
1 at 419.473 kb on - strand at 419.695 kb on - strand, within CSW01_16630 at 419.704 kb on - strand, within CSW01_16630 at 419.870 kb on + strand, within CSW01_16635 at 420.933 kb on + strand at 421.039 kb on + strand, within CSW01_16655 at 421.092 kb on + strand, within CSW01_16655 at 421.125 kb on + strand, within CSW01_16655 at 421.172 kb on + strand, within CSW01_16655 at 421.532 kb on - strand, within CSW01_16655 at 421.538 kb on + strand, within CSW01_16655 at 421.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 419,473 - +0.6 419,695 - CSW01_16630 0.60 -0.8 419,704 - CSW01_16630 0.65 -0.2 419,870 + CSW01_16635 0.62 +1.4 420,933 + +1.3 421,039 + CSW01_16655 0.11 -1.4 421,092 + CSW01_16655 0.19 -1.0 421,125 + CSW01_16655 0.24 +0.9 421,172 + CSW01_16655 0.31 -0.1 421,532 - CSW01_16655 0.85 -0.8 421,538 + CSW01_16655 0.86 -0.5 421,656 - +0.4
Or see this region's nucleotide sequence