Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15510 and CSW01_15515 are separated by 161 nucleotides CSW01_15515 and CSW01_15520 are separated by 130 nucleotides CSW01_15520 and CSW01_15525 are separated by 153 nucleotides CSW01_15525 and CSW01_15530 are separated by 0 nucleotides CSW01_15530 and CSW01_15535 are separated by 243 nucleotides
CSW01_15510: CSW01_15510 - deoxyguanosinetriphosphate triphosphohydrolase, at 318,142 to 319,350
_15510
CSW01_15515: CSW01_15515 - HNH endonuclease, at 319,512 to 320,216
_15515
CSW01_15520: CSW01_15520 - hypothetical protein, at 320,347 to 320,712
_15520
CSW01_15525: CSW01_15525 - type II toxin-antitoxin system RelE/ParE family toxin, at 320,866 to 321,201
_15525
CSW01_15530: CSW01_15530 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 321,202 to 321,444
_15530
CSW01_15535: CSW01_15535 - NUDIX hydrolase, at 321,688 to 322,074
_15535
Position (kb) 320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.358 kb on - strand at 319.358 kb on - strand at 319.412 kb on + strand at 319.414 kb on + strand at 319.503 kb on - strand at 319.670 kb on - strand, within CSW01_15515 at 319.681 kb on + strand, within CSW01_15515 at 319.936 kb on - strand, within CSW01_15515 at 319.951 kb on - strand, within CSW01_15515 at 319.987 kb on - strand, within CSW01_15515 at 319.993 kb on - strand, within CSW01_15515 at 319.998 kb on - strand, within CSW01_15515 at 320.030 kb on - strand, within CSW01_15515 at 320.037 kb on + strand, within CSW01_15515 at 320.116 kb on + strand, within CSW01_15515 at 320.242 kb on - strand at 320.271 kb on + strand at 320.306 kb on + strand at 320.406 kb on - strand, within CSW01_15520 at 320.511 kb on + strand, within CSW01_15520 at 320.551 kb on + strand, within CSW01_15520 at 320.554 kb on - strand, within CSW01_15520 at 320.559 kb on - strand, within CSW01_15520 at 320.559 kb on - strand, within CSW01_15520 at 320.705 kb on + strand at 320.713 kb on - strand at 320.723 kb on - strand at 320.723 kb on - strand at 321.661 kb on + strand at 321.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 319,358 - +0.3 319,358 - +0.7 319,412 + -0.5 319,414 + -1.3 319,503 - +0.8 319,670 - CSW01_15515 0.22 +0.4 319,681 + CSW01_15515 0.24 +1.5 319,936 - CSW01_15515 0.60 +0.4 319,951 - CSW01_15515 0.62 -2.2 319,987 - CSW01_15515 0.67 +0.6 319,993 - CSW01_15515 0.68 +0.1 319,998 - CSW01_15515 0.69 -1.8 320,030 - CSW01_15515 0.73 +0.5 320,037 + CSW01_15515 0.74 +1.9 320,116 + CSW01_15515 0.86 -1.4 320,242 - +0.3 320,271 + +0.5 320,306 + -0.5 320,406 - CSW01_15520 0.16 -2.2 320,511 + CSW01_15520 0.45 -1.1 320,551 + CSW01_15520 0.56 +0.0 320,554 - CSW01_15520 0.57 -0.9 320,559 - CSW01_15520 0.58 +0.3 320,559 - CSW01_15520 0.58 -3.3 320,705 + +1.3 320,713 - +1.0 320,723 - +0.0 320,723 - +0.1 321,661 + +0.1 321,669 - +0.2
Or see this region's nucleotide sequence