Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15015 and CSW01_15020 are separated by 250 nucleotides CSW01_15020 and CSW01_15025 are separated by 102 nucleotides CSW01_15025 and CSW01_15030 overlap by 50 nucleotides CSW01_15030 and CSW01_15035 are separated by 106 nucleotides
CSW01_15015: CSW01_15015 - anaerobic C4-dicarboxylate transporter, at 222,307 to 223,635
_15015
CSW01_15020: CSW01_15020 - NAD(+) synthetase, at 223,886 to 224,716
_15020
CSW01_15025: CSW01_15025 - nicotinate-nicotinamide nucleotide adenylyltransferase, at 224,819 to 225,346
_15025
CSW01_15030: CSW01_15030 - hypothetical protein, at 225,297 to 225,479
_15030
CSW01_15035: CSW01_15035 - transcriptional regulator, at 225,586 to 227,154
_15035
Position (kb) 223
224
225 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 222.925 kb on - strand, within CSW01_15015 at 222.938 kb on - strand, within CSW01_15015 at 223.010 kb on + strand, within CSW01_15015 at 223.012 kb on + strand, within CSW01_15015 at 223.032 kb on + strand, within CSW01_15015 at 223.145 kb on - strand, within CSW01_15015 at 223.182 kb on - strand, within CSW01_15015 at 223.318 kb on + strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.336 kb on + strand, within CSW01_15015 at 223.337 kb on - strand, within CSW01_15015 at 223.403 kb on - strand, within CSW01_15015 at 223.452 kb on + strand, within CSW01_15015 at 223.467 kb on + strand, within CSW01_15015 at 223.500 kb on - strand, within CSW01_15015 at 223.581 kb on + strand at 223.701 kb on + strand at 223.858 kb on - strand at 223.862 kb on + strand at 223.870 kb on - strand at 224.759 kb on - strand at 224.812 kb on + strand at 225.322 kb on + strand at 225.322 kb on + strand at 225.330 kb on - strand at 225.330 kb on - strand at 225.332 kb on + strand at 225.370 kb on - strand, within CSW01_15030 at 225.420 kb on - strand, within CSW01_15030 at 225.438 kb on + strand, within CSW01_15030 at 225.448 kb on - strand, within CSW01_15030 at 225.611 kb on + strand at 225.667 kb on + strand at 225.684 kb on + strand at 225.692 kb on - strand at 225.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 222,925 - CSW01_15015 0.47 +1.9 222,938 - CSW01_15015 0.47 +0.8 223,010 + CSW01_15015 0.53 -3.6 223,012 + CSW01_15015 0.53 -1.5 223,032 + CSW01_15015 0.55 -0.7 223,145 - CSW01_15015 0.63 +0.8 223,182 - CSW01_15015 0.66 +0.2 223,318 + CSW01_15015 0.76 +0.6 223,327 - CSW01_15015 0.77 +0.5 223,327 - CSW01_15015 0.77 -0.9 223,327 - CSW01_15015 0.77 -1.7 223,336 + CSW01_15015 0.77 +0.1 223,337 - CSW01_15015 0.78 +1.4 223,403 - CSW01_15015 0.82 -0.0 223,452 + CSW01_15015 0.86 -2.1 223,467 + CSW01_15015 0.87 +0.1 223,500 - CSW01_15015 0.90 +2.7 223,581 + -2.1 223,701 + -0.7 223,858 - +0.8 223,862 + -1.7 223,870 - -0.6 224,759 - -0.5 224,812 + +1.7 225,322 + +0.1 225,322 + -0.3 225,330 - -0.3 225,330 - -1.4 225,332 + +0.3 225,370 - CSW01_15030 0.40 +1.0 225,420 - CSW01_15030 0.67 +1.0 225,438 + CSW01_15030 0.77 +0.7 225,448 - CSW01_15030 0.83 +1.1 225,611 + +1.1 225,667 + -2.9 225,684 + -0.8 225,692 - +0.3 225,696 + +1.0
Or see this region's nucleotide sequence