Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_12035 and CSW01_12040 are separated by 186 nucleotides CSW01_12040 and CSW01_12045 are separated by 87 nucleotides CSW01_12045 and CSW01_12050 are separated by 12 nucleotides
CSW01_12035: CSW01_12035 - glutamate synthase small subunit, at 2,573,535 to 2,574,947
_12035
CSW01_12040: CSW01_12040 - DUF1499 domain-containing protein, at 2,575,134 to 2,575,568
_12040
CSW01_12045: CSW01_12045 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 2,575,656 to 2,576,351
_12045
CSW01_12050: CSW01_12050 - adenosylcobinamide-phosphate synthase, at 2,576,364 to 2,577,317
_12050
Position (kb)
2575
2576 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2574.345 kb on - strand, within CSW01_12035 at 2574.423 kb on + strand, within CSW01_12035 at 2574.423 kb on + strand, within CSW01_12035 at 2574.423 kb on + strand, within CSW01_12035 at 2574.599 kb on - strand, within CSW01_12035 at 2574.599 kb on - strand, within CSW01_12035 at 2574.618 kb on - strand, within CSW01_12035 at 2574.620 kb on + strand, within CSW01_12035 at 2574.666 kb on + strand, within CSW01_12035 at 2574.700 kb on - strand, within CSW01_12035 at 2574.700 kb on - strand, within CSW01_12035 at 2574.700 kb on - strand, within CSW01_12035 at 2574.754 kb on - strand, within CSW01_12035 at 2574.759 kb on - strand, within CSW01_12035 at 2574.759 kb on - strand, within CSW01_12035 at 2574.780 kb on - strand, within CSW01_12035 at 2574.821 kb on + strand at 2574.879 kb on - strand at 2574.905 kb on + strand at 2574.905 kb on + strand at 2574.912 kb on + strand at 2574.942 kb on - strand at 2574.954 kb on + strand at 2575.033 kb on + strand at 2575.052 kb on + strand at 2575.078 kb on - strand at 2575.108 kb on + strand at 2575.108 kb on + strand at 2575.117 kb on + strand at 2575.125 kb on - strand at 2575.125 kb on - strand at 2575.211 kb on - strand, within CSW01_12040 at 2575.279 kb on + strand, within CSW01_12040 at 2575.307 kb on + strand, within CSW01_12040 at 2575.320 kb on - strand, within CSW01_12040 at 2575.390 kb on - strand, within CSW01_12040 at 2575.399 kb on + strand, within CSW01_12040 at 2575.407 kb on + strand, within CSW01_12040 at 2575.407 kb on - strand, within CSW01_12040 at 2575.513 kb on + strand, within CSW01_12040 at 2575.516 kb on + strand, within CSW01_12040 at 2575.516 kb on + strand, within CSW01_12040 at 2575.516 kb on + strand, within CSW01_12040 at 2575.521 kb on - strand, within CSW01_12040 at 2575.524 kb on - strand, within CSW01_12040 at 2575.524 kb on - strand, within CSW01_12040 at 2575.524 kb on - strand, within CSW01_12040 at 2575.563 kb on + strand at 2575.563 kb on + strand at 2575.563 kb on + strand at 2575.571 kb on - strand at 2575.578 kb on + strand at 2575.586 kb on - strand at 2575.586 kb on - strand at 2575.607 kb on + strand at 2575.615 kb on - strand at 2576.348 kb on - strand at 2576.350 kb on - strand at 2576.359 kb on + strand at 2576.405 kb on + strand at 2576.416 kb on + strand at 2576.546 kb on + strand, within CSW01_12050 at 2576.558 kb on + strand, within CSW01_12050 at 2576.566 kb on - strand, within CSW01_12050 at 2576.566 kb on - strand, within CSW01_12050
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 2,574,345 - CSW01_12035 0.57 -0.3 2,574,423 + CSW01_12035 0.63 +1.3 2,574,423 + CSW01_12035 0.63 +0.1 2,574,423 + CSW01_12035 0.63 -1.5 2,574,599 - CSW01_12035 0.75 -0.3 2,574,599 - CSW01_12035 0.75 -1.0 2,574,618 - CSW01_12035 0.77 +0.4 2,574,620 + CSW01_12035 0.77 -0.5 2,574,666 + CSW01_12035 0.80 +1.7 2,574,700 - CSW01_12035 0.82 +0.4 2,574,700 - CSW01_12035 0.82 -0.3 2,574,700 - CSW01_12035 0.82 +0.9 2,574,754 - CSW01_12035 0.86 -2.5 2,574,759 - CSW01_12035 0.87 -1.2 2,574,759 - CSW01_12035 0.87 -0.1 2,574,780 - CSW01_12035 0.88 -0.1 2,574,821 + -2.7 2,574,879 - -0.9 2,574,905 + +1.8 2,574,905 + +0.6 2,574,912 + +0.3 2,574,942 - +0.7 2,574,954 + -0.5 2,575,033 + +0.7 2,575,052 + +0.6 2,575,078 - -0.2 2,575,108 + +0.9 2,575,108 + +0.2 2,575,117 + +0.6 2,575,125 - -0.3 2,575,125 - -2.6 2,575,211 - CSW01_12040 0.18 -2.5 2,575,279 + CSW01_12040 0.33 -3.0 2,575,307 + CSW01_12040 0.40 +1.0 2,575,320 - CSW01_12040 0.43 -2.4 2,575,390 - CSW01_12040 0.59 +0.9 2,575,399 + CSW01_12040 0.61 -0.1 2,575,407 + CSW01_12040 0.63 +0.8 2,575,407 - CSW01_12040 0.63 +1.0 2,575,513 + CSW01_12040 0.87 +1.2 2,575,516 + CSW01_12040 0.88 +0.6 2,575,516 + CSW01_12040 0.88 +0.4 2,575,516 + CSW01_12040 0.88 +0.8 2,575,521 - CSW01_12040 0.89 +1.0 2,575,524 - CSW01_12040 0.90 +0.1 2,575,524 - CSW01_12040 0.90 -2.3 2,575,524 - CSW01_12040 0.90 +1.7 2,575,563 + +1.4 2,575,563 + +0.2 2,575,563 + +0.9 2,575,571 - +1.5 2,575,578 + -1.6 2,575,586 - -0.1 2,575,586 - -0.1 2,575,607 + -0.5 2,575,615 - -2.2 2,576,348 - -0.3 2,576,350 - +0.5 2,576,359 + -0.1 2,576,405 + -0.1 2,576,416 + +0.5 2,576,546 + CSW01_12050 0.19 -0.2 2,576,558 + CSW01_12050 0.20 -3.4 2,576,566 - CSW01_12050 0.21 -0.1 2,576,566 - CSW01_12050 0.21 -2.6
Or see this region's nucleotide sequence