Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_05885 and CSW01_05890 are separated by 8 nucleotides CSW01_05890 and CSW01_05895 are separated by 433 nucleotides CSW01_05895 and CSW01_05900 are separated by 111 nucleotides CSW01_05900 and CSW01_05905 are separated by 137 nucleotides
CSW01_05885: CSW01_05885 - gonadoliberin III, at 1,231,383 to 1,232,891
_05885
CSW01_05890: CSW01_05890 - hypothetical protein, at 1,232,900 to 1,233,322
_05890
CSW01_05895: CSW01_05895 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 1,233,756 to 1,234,727
_05895
CSW01_05900: CSW01_05900 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 1,234,839 to 1,235,567
_05900
CSW01_05905: CSW01_05905 - DUF72 domain-containing protein, at 1,235,705 to 1,236,574
_05905
Position (kb)
1233
1234
1235 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1232.799 kb on - strand at 1232.957 kb on + strand, within CSW01_05890 at 1232.967 kb on + strand, within CSW01_05890 at 1233.019 kb on - strand, within CSW01_05890 at 1233.028 kb on + strand, within CSW01_05890 at 1233.034 kb on - strand, within CSW01_05890 at 1233.043 kb on + strand, within CSW01_05890 at 1233.078 kb on + strand, within CSW01_05890 at 1233.086 kb on - strand, within CSW01_05890 at 1233.127 kb on + strand, within CSW01_05890 at 1233.147 kb on + strand, within CSW01_05890 at 1233.147 kb on + strand, within CSW01_05890 at 1233.316 kb on + strand at 1233.350 kb on + strand at 1233.358 kb on - strand at 1233.557 kb on + strand at 1233.572 kb on + strand at 1233.580 kb on - strand at 1233.682 kb on + strand at 1233.722 kb on - strand at 1233.747 kb on - strand at 1233.801 kb on + strand at 1233.810 kb on + strand at 1233.835 kb on + strand at 1233.864 kb on - strand, within CSW01_05895 at 1233.871 kb on + strand, within CSW01_05895 at 1233.871 kb on + strand, within CSW01_05895 at 1233.871 kb on + strand, within CSW01_05895 at 1233.871 kb on + strand, within CSW01_05895 at 1233.879 kb on - strand, within CSW01_05895 at 1233.879 kb on - strand, within CSW01_05895 at 1233.879 kb on - strand, within CSW01_05895 at 1233.879 kb on - strand, within CSW01_05895 at 1233.932 kb on - strand, within CSW01_05895 at 1234.170 kb on - strand, within CSW01_05895 at 1234.170 kb on - strand, within CSW01_05895 at 1234.181 kb on + strand, within CSW01_05895 at 1234.189 kb on - strand, within CSW01_05895 at 1234.189 kb on - strand, within CSW01_05895 at 1234.189 kb on - strand, within CSW01_05895 at 1234.189 kb on - strand, within CSW01_05895 at 1234.222 kb on - strand, within CSW01_05895 at 1234.262 kb on + strand, within CSW01_05895 at 1234.271 kb on + strand, within CSW01_05895 at 1234.271 kb on + strand, within CSW01_05895 at 1234.284 kb on - strand, within CSW01_05895 at 1234.305 kb on - strand, within CSW01_05895 at 1234.446 kb on + strand, within CSW01_05895 at 1234.474 kb on - strand, within CSW01_05895 at 1234.482 kb on - strand, within CSW01_05895 at 1234.482 kb on - strand, within CSW01_05895 at 1234.482 kb on - strand, within CSW01_05895 at 1234.616 kb on - strand, within CSW01_05895 at 1234.623 kb on + strand, within CSW01_05895 at 1234.623 kb on + strand, within CSW01_05895 at 1234.623 kb on + strand, within CSW01_05895 at 1234.660 kb on + strand at 1234.709 kb on - strand at 1234.742 kb on + strand at 1235.234 kb on + strand, within CSW01_05900 at 1235.237 kb on - strand, within CSW01_05900 at 1235.265 kb on - strand, within CSW01_05900 at 1235.265 kb on - strand, within CSW01_05900 at 1235.265 kb on - strand, within CSW01_05900 at 1235.475 kb on - strand, within CSW01_05900 at 1235.504 kb on + strand at 1235.504 kb on + strand at 1235.512 kb on - strand at 1235.574 kb on + strand at 1235.574 kb on + strand at 1235.580 kb on - strand at 1235.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 1,232,799 - -2.1 1,232,957 + CSW01_05890 0.13 -2.7 1,232,967 + CSW01_05890 0.16 +0.8 1,233,019 - CSW01_05890 0.28 +0.4 1,233,028 + CSW01_05890 0.30 -1.7 1,233,034 - CSW01_05890 0.32 +0.4 1,233,043 + CSW01_05890 0.34 -1.1 1,233,078 + CSW01_05890 0.42 -0.3 1,233,086 - CSW01_05890 0.44 -1.4 1,233,127 + CSW01_05890 0.54 -0.9 1,233,147 + CSW01_05890 0.58 +1.1 1,233,147 + CSW01_05890 0.58 +0.4 1,233,316 + +0.3 1,233,350 + -0.3 1,233,358 - -1.6 1,233,557 + +0.1 1,233,572 + -2.0 1,233,580 - +0.1 1,233,682 + -0.4 1,233,722 - +0.6 1,233,747 - +0.6 1,233,801 + +1.8 1,233,810 + +1.6 1,233,835 + +0.9 1,233,864 - CSW01_05895 0.11 -2.4 1,233,871 + CSW01_05895 0.12 -0.4 1,233,871 + CSW01_05895 0.12 -1.2 1,233,871 + CSW01_05895 0.12 +0.6 1,233,871 + CSW01_05895 0.12 +0.5 1,233,879 - CSW01_05895 0.13 +2.6 1,233,879 - CSW01_05895 0.13 -0.1 1,233,879 - CSW01_05895 0.13 +0.8 1,233,879 - CSW01_05895 0.13 +1.6 1,233,932 - CSW01_05895 0.18 +0.3 1,234,170 - CSW01_05895 0.43 +1.5 1,234,170 - CSW01_05895 0.43 -3.1 1,234,181 + CSW01_05895 0.44 +0.0 1,234,189 - CSW01_05895 0.45 +0.8 1,234,189 - CSW01_05895 0.45 -2.6 1,234,189 - CSW01_05895 0.45 +0.3 1,234,189 - CSW01_05895 0.45 +0.7 1,234,222 - CSW01_05895 0.48 +1.2 1,234,262 + CSW01_05895 0.52 +0.6 1,234,271 + CSW01_05895 0.53 -0.3 1,234,271 + CSW01_05895 0.53 -2.1 1,234,284 - CSW01_05895 0.54 +0.9 1,234,305 - CSW01_05895 0.56 -0.0 1,234,446 + CSW01_05895 0.71 +0.3 1,234,474 - CSW01_05895 0.74 +0.0 1,234,482 - CSW01_05895 0.75 +0.1 1,234,482 - CSW01_05895 0.75 -1.1 1,234,482 - CSW01_05895 0.75 +2.2 1,234,616 - CSW01_05895 0.88 -1.0 1,234,623 + CSW01_05895 0.89 -3.5 1,234,623 + CSW01_05895 0.89 -2.5 1,234,623 + CSW01_05895 0.89 +0.7 1,234,660 + -2.0 1,234,709 - +1.4 1,234,742 + +0.4 1,235,234 + CSW01_05900 0.54 +0.4 1,235,237 - CSW01_05900 0.55 +0.8 1,235,265 - CSW01_05900 0.58 -0.8 1,235,265 - CSW01_05900 0.58 -0.9 1,235,265 - CSW01_05900 0.58 -0.2 1,235,475 - CSW01_05900 0.87 -3.6 1,235,504 + -2.1 1,235,504 + -2.1 1,235,512 - -0.7 1,235,574 + -0.5 1,235,574 + +0.0 1,235,580 - -1.5 1,235,719 + -0.2
Or see this region's nucleotide sequence