Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03875 and CSW01_03880 are separated by 69 nucleotides CSW01_03880 and CSW01_03885 are separated by 26 nucleotides CSW01_03885 and CSW01_03890 are separated by 36 nucleotides CSW01_03890 and CSW01_03895 are separated by 60 nucleotides CSW01_03895 and CSW01_03900 are separated by 48 nucleotides
CSW01_03875: CSW01_03875 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 798,550 to 799,284
_03875
CSW01_03880: CSW01_03880 - HTH-type transcriptional regulator IscR, at 799,354 to 799,920
_03880
CSW01_03885: CSW01_03885 - IscS subfamily cysteine desulfurase, at 799,947 to 801,161
_03885
CSW01_03890: CSW01_03890 - Fe-S cluster assembly scaffold IscU, at 801,198 to 801,581
_03890
CSW01_03895: CSW01_03895 - iron-sulfur cluster assembly protein IscA, at 801,642 to 801,965
_03895
CSW01_03900: CSW01_03900 - co-chaperone protein HscB, at 802,014 to 802,529
_03900
Position (kb) 799
800
801
802 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 798.952 kb on - strand, within CSW01_03875 at 798.975 kb on - strand, within CSW01_03875 at 798.989 kb on - strand, within CSW01_03875 at 799.010 kb on + strand, within CSW01_03875 at 799.010 kb on + strand, within CSW01_03875 at 799.018 kb on - strand, within CSW01_03875 at 799.026 kb on + strand, within CSW01_03875 at 799.056 kb on + strand, within CSW01_03875 at 799.064 kb on - strand, within CSW01_03875 at 799.068 kb on + strand, within CSW01_03875 at 799.126 kb on + strand, within CSW01_03875 at 799.370 kb on + strand at 799.713 kb on + strand, within CSW01_03880 at 799.844 kb on + strand, within CSW01_03880 at 799.927 kb on + strand at 801.155 kb on + strand at 801.155 kb on + strand at 801.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 798,952 - CSW01_03875 0.55 +1.2 798,975 - CSW01_03875 0.58 +1.0 798,989 - CSW01_03875 0.60 -1.2 799,010 + CSW01_03875 0.63 -0.0 799,010 + CSW01_03875 0.63 -0.1 799,018 - CSW01_03875 0.64 -2.0 799,026 + CSW01_03875 0.65 +0.3 799,056 + CSW01_03875 0.69 -1.9 799,064 - CSW01_03875 0.70 +0.9 799,068 + CSW01_03875 0.70 +1.2 799,126 + CSW01_03875 0.78 +1.0 799,370 + -1.9 799,713 + CSW01_03880 0.63 -3.7 799,844 + CSW01_03880 0.86 -2.4 799,927 + +0.7 801,155 + +1.0 801,155 + +1.9 801,595 + -0.0
Or see this region's nucleotide sequence