Experiment: ICP1_phage_2.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_02295 and CSW01_02300 are separated by 13 nucleotides CSW01_02300 and CSW01_02305 overlap by 13 nucleotides CSW01_02305 and CSW01_02310 are separated by 12 nucleotides CSW01_02310 and CSW01_02315 are separated by 148 nucleotides
CSW01_02295: CSW01_02295 - GTPase ObgE, at 466,005 to 467,177
_02295
CSW01_02300: CSW01_02300 - hypothetical protein, at 467,191 to 468,060
_02300
CSW01_02305: CSW01_02305 - hypothetical protein, at 468,048 to 468,533
_02305
CSW01_02310: CSW01_02310 - type 3 dihydrofolate reductase, at 468,546 to 469,043
_02310
CSW01_02315: CSW01_02315 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 469,192 to 470,001
_02315
Position (kb) 468
469 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 467.069 kb on - strand at 467.160 kb on + strand at 467.255 kb on + strand at 467.263 kb on - strand at 467.263 kb on - strand at 467.273 kb on - strand at 467.284 kb on - strand, within CSW01_02300 at 467.344 kb on + strand, within CSW01_02300 at 467.352 kb on - strand, within CSW01_02300 at 467.352 kb on - strand, within CSW01_02300 at 467.464 kb on - strand, within CSW01_02300 at 467.489 kb on + strand, within CSW01_02300 at 467.489 kb on + strand, within CSW01_02300 at 467.489 kb on + strand, within CSW01_02300 at 467.490 kb on + strand, within CSW01_02300 at 467.510 kb on + strand, within CSW01_02300 at 467.594 kb on + strand, within CSW01_02300 at 467.594 kb on + strand, within CSW01_02300 at 467.602 kb on - strand, within CSW01_02300 at 467.624 kb on + strand, within CSW01_02300 at 467.632 kb on - strand, within CSW01_02300 at 467.632 kb on - strand, within CSW01_02300 at 467.668 kb on - strand, within CSW01_02300 at 467.688 kb on - strand, within CSW01_02300 at 467.688 kb on - strand, within CSW01_02300 at 467.704 kb on + strand, within CSW01_02300 at 467.893 kb on + strand, within CSW01_02300 at 467.922 kb on + strand, within CSW01_02300 at 467.943 kb on + strand, within CSW01_02300 at 467.951 kb on - strand, within CSW01_02300 at 468.007 kb on - strand at 468.097 kb on - strand, within CSW01_02305 at 468.174 kb on + strand, within CSW01_02305 at 468.181 kb on + strand, within CSW01_02305 at 468.239 kb on - strand, within CSW01_02305 at 468.328 kb on - strand, within CSW01_02305 at 469.232 kb on + strand at 469.237 kb on + strand at 469.272 kb on - strand at 469.300 kb on + strand, within CSW01_02315 at 469.393 kb on - strand, within CSW01_02315 at 469.473 kb on - strand, within CSW01_02315 at 469.502 kb on - strand, within CSW01_02315 at 469.503 kb on + strand, within CSW01_02315 at 469.528 kb on + strand, within CSW01_02315 at 469.528 kb on + strand, within CSW01_02315
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep2 remove 467,069 - +0.9 467,160 + +0.1 467,255 + +0.0 467,263 - +0.9 467,263 - -0.0 467,273 - -3.2 467,284 - CSW01_02300 0.11 +1.3 467,344 + CSW01_02300 0.18 -0.5 467,352 - CSW01_02300 0.19 -0.9 467,352 - CSW01_02300 0.19 +0.8 467,464 - CSW01_02300 0.31 -4.0 467,489 + CSW01_02300 0.34 +3.0 467,489 + CSW01_02300 0.34 +1.8 467,489 + CSW01_02300 0.34 -1.7 467,490 + CSW01_02300 0.34 +1.2 467,510 + CSW01_02300 0.37 +1.5 467,594 + CSW01_02300 0.46 +0.4 467,594 + CSW01_02300 0.46 +0.8 467,602 - CSW01_02300 0.47 -0.3 467,624 + CSW01_02300 0.50 -0.4 467,632 - CSW01_02300 0.51 -2.3 467,632 - CSW01_02300 0.51 +0.6 467,668 - CSW01_02300 0.55 +0.9 467,688 - CSW01_02300 0.57 -2.2 467,688 - CSW01_02300 0.57 -2.4 467,704 + CSW01_02300 0.59 -1.5 467,893 + CSW01_02300 0.81 +0.2 467,922 + CSW01_02300 0.84 -4.2 467,943 + CSW01_02300 0.86 +0.9 467,951 - CSW01_02300 0.87 +0.7 468,007 - -1.1 468,097 - CSW01_02305 0.10 -2.9 468,174 + CSW01_02305 0.26 -1.7 468,181 + CSW01_02305 0.27 -1.0 468,239 - CSW01_02305 0.39 -0.6 468,328 - CSW01_02305 0.58 +0.1 469,232 + -0.4 469,237 + -0.8 469,272 - +1.4 469,300 + CSW01_02315 0.13 -0.1 469,393 - CSW01_02315 0.25 +1.0 469,473 - CSW01_02315 0.35 -3.6 469,502 - CSW01_02315 0.38 -1.6 469,503 + CSW01_02315 0.38 -1.5 469,528 + CSW01_02315 0.41 -0.9 469,528 + CSW01_02315 0.41 -0.3
Or see this region's nucleotide sequence