Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02935 and AO353_02940 are separated by 388 nucleotides AO353_02940 and AO353_02945 are separated by 165 nucleotides AO353_02945 and AO353_02950 are separated by 320 nucleotides AO353_02950 and AO353_02955 are separated by 19 nucleotides
AO353_02935: AO353_02935 - ribose-phosphate pyrophosphokinase, at 624,348 to 624,932
_02935
AO353_02940: AO353_02940 - DNA-binding protein, at 625,321 to 625,647
_02940
AO353_02945: AO353_02945 - magnesium transporter, at 625,813 to 627,255
_02945
AO353_02950: AO353_02950 - cell envelope protein SmpA, at 627,576 to 628,088
_02950
AO353_02955: AO353_02955 - tRNA-Arg, at 628,108 to 628,184
_02955
Position (kb) 625
626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 624.913 kb on + strand at 624.913 kb on + strand at 624.914 kb on - strand at 624.914 kb on - strand at 624.914 kb on - strand at 624.914 kb on - strand at 624.914 kb on - strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.917 kb on + strand at 624.918 kb on - strand at 624.918 kb on - strand at 624.918 kb on - strand at 624.918 kb on - strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.937 kb on - strand at 624.938 kb on + strand at 624.938 kb on + strand at 624.939 kb on - strand at 624.939 kb on - strand at 624.985 kb on + strand at 624.985 kb on + strand at 624.992 kb on + strand at 624.992 kb on + strand at 624.993 kb on - strand at 624.999 kb on + strand at 625.000 kb on - strand at 625.023 kb on + strand at 625.023 kb on + strand at 625.023 kb on + strand at 625.024 kb on - strand at 625.087 kb on - strand at 625.087 kb on - strand at 625.087 kb on - strand at 625.097 kb on + strand at 625.098 kb on - strand at 625.164 kb on + strand at 625.164 kb on + strand at 625.164 kb on + strand at 625.164 kb on + strand at 625.165 kb on - strand at 625.165 kb on - strand at 625.165 kb on - strand at 625.223 kb on + strand at 625.224 kb on - strand at 625.262 kb on + strand at 625.262 kb on + strand at 625.262 kb on + strand at 625.262 kb on + strand at 625.263 kb on - strand at 625.263 kb on - strand at 625.263 kb on - strand at 625.263 kb on - strand at 625.263 kb on - strand at 625.263 kb on - strand at 625.319 kb on - strand at 625.319 kb on - strand at 625.321 kb on - strand at 625.321 kb on - strand at 625.345 kb on + strand at 625.357 kb on - strand, within AO353_02940 at 625.376 kb on + strand, within AO353_02940 at 625.377 kb on - strand, within AO353_02940 at 625.377 kb on - strand, within AO353_02940 at 625.476 kb on + strand, within AO353_02940 at 625.476 kb on + strand, within AO353_02940 at 625.476 kb on + strand, within AO353_02940 at 625.666 kb on - strand at 625.714 kb on + strand at 625.714 kb on + strand at 625.714 kb on + strand at 625.714 kb on + strand at 625.714 kb on + strand at 625.715 kb on - strand at 625.715 kb on - strand at 625.715 kb on - strand at 625.715 kb on - strand at 625.745 kb on + strand at 625.745 kb on + strand at 625.745 kb on + strand at 625.745 kb on + strand at 625.746 kb on - strand at 625.746 kb on - strand at 625.746 kb on - strand at 625.751 kb on + strand at 625.752 kb on - strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.754 kb on + strand at 625.755 kb on - strand at 625.755 kb on - strand at 625.755 kb on - strand at 625.755 kb on - strand at 625.755 kb on - strand at 625.755 kb on - strand at 625.806 kb on + strand at 625.806 kb on + strand at 625.806 kb on + strand at 625.806 kb on + strand at 625.806 kb on + strand at 625.807 kb on - strand at 625.807 kb on - strand at 625.807 kb on - strand at 625.807 kb on - strand at 625.807 kb on - strand at 625.807 kb on - strand at 626.019 kb on - strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.027 kb on + strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.028 kb on - strand, within AO353_02945 at 626.109 kb on + strand, within AO353_02945 at 626.110 kb on - strand, within AO353_02945 at 626.157 kb on + strand, within AO353_02945 at 626.157 kb on + strand, within AO353_02945 at 626.158 kb on - strand, within AO353_02945 at 626.178 kb on + strand, within AO353_02945 at 626.235 kb on + strand, within AO353_02945 at 626.343 kb on + strand, within AO353_02945 at 626.344 kb on - strand, within AO353_02945 at 626.344 kb on - strand, within AO353_02945 at 626.484 kb on - strand, within AO353_02945 at 626.510 kb on + strand, within AO353_02945 at 626.511 kb on - strand, within AO353_02945 at 626.630 kb on + strand, within AO353_02945 at 626.630 kb on + strand, within AO353_02945 at 626.687 kb on + strand, within AO353_02945 at 626.687 kb on + strand, within AO353_02945 at 626.687 kb on + strand, within AO353_02945 at 626.688 kb on - strand, within AO353_02945 at 626.771 kb on + strand, within AO353_02945 at 626.956 kb on + strand, within AO353_02945 at 626.957 kb on - strand, within AO353_02945 at 626.957 kb on - strand, within AO353_02945 at 627.044 kb on + strand, within AO353_02945 at 627.044 kb on + strand, within AO353_02945 at 627.044 kb on + strand, within AO353_02945 at 627.045 kb on - strand, within AO353_02945 at 627.045 kb on - strand, within AO353_02945 at 627.045 kb on - strand, within AO353_02945 at 627.045 kb on - strand, within AO353_02945 at 627.045 kb on - strand, within AO353_02945 at 627.255 kb on + strand at 627.319 kb on + strand at 627.319 kb on + strand at 627.319 kb on + strand at 627.320 kb on - strand at 627.320 kb on - strand at 627.320 kb on - strand at 627.340 kb on + strand at 627.340 kb on + strand at 627.340 kb on + strand at 627.340 kb on + strand at 627.340 kb on + strand at 627.340 kb on + strand at 627.341 kb on - strand at 627.348 kb on - strand at 627.361 kb on + strand at 627.399 kb on + strand at 627.399 kb on + strand at 627.399 kb on + strand at 627.399 kb on + strand at 627.400 kb on - strand at 627.400 kb on - strand at 627.418 kb on - strand at 627.418 kb on - strand at 627.418 kb on - strand at 627.459 kb on - strand at 627.535 kb on + strand at 627.535 kb on + strand at 627.536 kb on - strand at 627.628 kb on + strand, within AO353_02950 at 627.628 kb on + strand, within AO353_02950 at 627.628 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.684 kb on + strand, within AO353_02950 at 627.685 kb on - strand, within AO353_02950 at 627.685 kb on - strand, within AO353_02950 at 627.685 kb on - strand, within AO353_02950 at 627.685 kb on - strand, within AO353_02950 at 627.685 kb on - strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.735 kb on + strand, within AO353_02950 at 627.736 kb on - strand, within AO353_02950 at 627.736 kb on - strand, within AO353_02950 at 627.736 kb on - strand, within AO353_02950 at 627.736 kb on - strand, within AO353_02950 at 627.766 kb on + strand, within AO353_02950 at 627.766 kb on + strand, within AO353_02950 at 627.766 kb on + strand, within AO353_02950 at 627.767 kb on - strand, within AO353_02950 at 627.767 kb on - strand, within AO353_02950 at 627.767 kb on - strand, within AO353_02950 at 627.803 kb on + strand, within AO353_02950 at 627.803 kb on + strand, within AO353_02950 at 627.804 kb on - strand, within AO353_02950 at 627.804 kb on - strand, within AO353_02950 at 627.950 kb on + strand, within AO353_02950 at 627.950 kb on + strand, within AO353_02950 at 627.950 kb on + strand, within AO353_02950 at 627.951 kb on - strand, within AO353_02950 at 627.951 kb on - strand, within AO353_02950 at 627.951 kb on - strand, within AO353_02950 at 627.990 kb on + strand, within AO353_02950 at 627.990 kb on + strand, within AO353_02950 at 627.991 kb on - strand, within AO353_02950 at 627.991 kb on - strand, within AO353_02950 at 627.997 kb on - strand, within AO353_02950 at 628.051 kb on + strand at 628.052 kb on - strand at 628.052 kb on - strand at 628.052 kb on - strand at 628.052 kb on - strand at 628.052 kb on - strand at 628.052 kb on - strand at 628.159 kb on - strand, within AO353_02955 at 628.159 kb on - strand, within AO353_02955 at 628.229 kb on - strand at 628.229 kb on - strand at 628.229 kb on - strand at 628.229 kb on - strand at 628.229 kb on - strand at 628.238 kb on - strand at 628.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 624,913 + -0.3 624,913 + -2.3 624,914 - +1.8 624,914 - +1.3 624,914 - -1.3 624,914 - -0.8 624,914 - -1.9 624,917 + -1.1 624,917 + -0.3 624,917 + -1.3 624,917 + -1.1 624,917 + -0.8 624,917 + -0.8 624,917 + -0.7 624,917 + -1.7 624,917 + -0.7 624,917 + -1.3 624,917 + -1.6 624,917 + -1.9 624,917 + -1.3 624,917 + +0.1 624,917 + -2.3 624,917 + -1.6 624,917 + -2.1 624,917 + -1.1 624,917 + -0.5 624,918 - -1.1 624,918 - -2.5 624,918 - +1.4 624,918 - +0.5 624,936 + -0.1 624,936 + -1.6 624,936 + -1.2 624,936 + -0.5 624,936 + +0.4 624,936 + +0.9 624,936 + -0.1 624,936 + +1.1 624,936 + -0.9 624,936 + -0.4 624,936 + -1.1 624,936 + -1.4 624,936 + -0.4 624,936 + -1.9 624,936 + -0.3 624,936 + -1.4 624,936 + -1.2 624,936 + -0.7 624,936 + -0.4 624,936 + -1.1 624,936 + +0.7 624,936 + +3.2 624,936 + -2.8 624,937 - +0.9 624,937 - -2.4 624,937 - -1.1 624,937 - +0.7 624,937 - +1.1 624,937 - -0.9 624,937 - +0.7 624,937 - +1.2 624,937 - +1.7 624,937 - +2.4 624,937 - -0.9 624,937 - +0.9 624,937 - -1.7 624,937 - -0.1 624,937 - +0.3 624,937 - +0.6 624,938 + +0.1 624,938 + -1.4 624,939 - -0.6 624,939 - -0.2 624,985 + -1.9 624,985 + -2.1 624,992 + +0.3 624,992 + -1.1 624,993 - -0.7 624,999 + +0.9 625,000 - +2.5 625,023 + +0.9 625,023 + +1.7 625,023 + -0.0 625,024 - -1.7 625,087 - -0.3 625,087 - +1.7 625,087 - -0.9 625,097 + -1.4 625,098 - -1.4 625,164 + -1.9 625,164 + -1.2 625,164 + -0.7 625,164 + -1.1 625,165 - -2.5 625,165 - +1.1 625,165 - +0.2 625,223 + +0.6 625,224 - +2.0 625,262 + +0.3 625,262 + -1.1 625,262 + -0.2 625,262 + -0.9 625,263 - +1.9 625,263 - +2.3 625,263 - +0.6 625,263 - -2.1 625,263 - +3.4 625,263 - -1.7 625,319 - +0.9 625,319 - -1.1 625,321 - +2.7 625,321 - +3.2 625,345 + +0.9 625,357 - AO353_02940 0.11 +0.4 625,376 + AO353_02940 0.17 +2.7 625,377 - AO353_02940 0.17 +3.6 625,377 - AO353_02940 0.17 +1.4 625,476 + AO353_02940 0.47 +2.9 625,476 + AO353_02940 0.47 +0.3 625,476 + AO353_02940 0.47 -0.1 625,666 - +3.5 625,714 + +0.5 625,714 + +0.3 625,714 + +0.9 625,714 + +0.9 625,714 + +1.8 625,715 - +0.1 625,715 - +0.6 625,715 - +1.7 625,715 - -0.8 625,745 + +1.5 625,745 + -1.0 625,745 + -1.1 625,745 + +0.4 625,746 - +1.7 625,746 - +1.2 625,746 - -0.4 625,751 + -0.5 625,752 - +2.0 625,754 + -1.9 625,754 + -1.6 625,754 + -1.0 625,754 + -0.3 625,754 + -0.1 625,754 + +0.1 625,754 + +0.3 625,754 + +1.4 625,754 + -0.8 625,754 + -2.3 625,754 + -2.0 625,754 + +1.2 625,754 + +1.2 625,754 + -0.7 625,755 - -0.1 625,755 - +0.9 625,755 - -1.2 625,755 - +0.1 625,755 - +0.7 625,755 - +1.3 625,806 + -0.1 625,806 + -1.2 625,806 + -0.1 625,806 + -0.4 625,806 + +0.7 625,807 - +0.5 625,807 - -1.1 625,807 - +1.1 625,807 - +1.2 625,807 - +1.7 625,807 - +2.4 626,019 - AO353_02945 0.14 -2.3 626,027 + AO353_02945 0.15 +0.9 626,027 + AO353_02945 0.15 -1.7 626,027 + AO353_02945 0.15 -1.5 626,027 + AO353_02945 0.15 -1.7 626,027 + AO353_02945 0.15 -0.3 626,027 + AO353_02945 0.15 -1.7 626,027 + AO353_02945 0.15 -2.3 626,027 + AO353_02945 0.15 +0.3 626,028 - AO353_02945 0.15 -3.6 626,028 - AO353_02945 0.15 -0.7 626,028 - AO353_02945 0.15 -0.1 626,028 - AO353_02945 0.15 -1.7 626,028 - AO353_02945 0.15 -0.4 626,028 - AO353_02945 0.15 -1.7 626,109 + AO353_02945 0.21 -1.5 626,110 - AO353_02945 0.21 -2.4 626,157 + AO353_02945 0.24 -2.3 626,157 + AO353_02945 0.24 -1.1 626,158 - AO353_02945 0.24 -1.1 626,178 + AO353_02945 0.25 -0.7 626,235 + AO353_02945 0.29 -0.9 626,343 + AO353_02945 0.37 -1.4 626,344 - AO353_02945 0.37 -1.9 626,344 - AO353_02945 0.37 -1.4 626,484 - AO353_02945 0.47 -1.1 626,510 + AO353_02945 0.48 -2.3 626,511 - AO353_02945 0.48 -2.7 626,630 + AO353_02945 0.57 -1.1 626,630 + AO353_02945 0.57 -2.3 626,687 + AO353_02945 0.61 -2.3 626,687 + AO353_02945 0.61 -0.5 626,687 + AO353_02945 0.61 +0.1 626,688 - AO353_02945 0.61 -2.3 626,771 + AO353_02945 0.66 -2.7 626,956 + AO353_02945 0.79 -0.7 626,957 - AO353_02945 0.79 +0.3 626,957 - AO353_02945 0.79 -1.3 627,044 + AO353_02945 0.85 -0.7 627,044 + AO353_02945 0.85 -0.5 627,044 + AO353_02945 0.85 -0.3 627,045 - AO353_02945 0.85 -0.1 627,045 - AO353_02945 0.85 +2.5 627,045 - AO353_02945 0.85 -0.7 627,045 - AO353_02945 0.85 -0.7 627,045 - AO353_02945 0.85 -1.8 627,255 + -1.4 627,319 + -1.4 627,319 + -0.1 627,319 + -0.1 627,320 - +0.2 627,320 - +0.5 627,320 - +1.2 627,340 + +0.9 627,340 + +0.7 627,340 + +0.4 627,340 + -0.7 627,340 + +0.9 627,340 + -2.5 627,341 - -1.9 627,348 - -0.8 627,361 + -1.7 627,399 + -0.5 627,399 + +1.2 627,399 + +2.4 627,399 + -0.1 627,400 - -0.7 627,400 - -0.3 627,418 - +0.9 627,418 - +1.9 627,418 - -2.1 627,459 - -1.7 627,535 + -0.7 627,535 + -0.1 627,536 - +0.6 627,628 + AO353_02950 0.10 +1.1 627,628 + AO353_02950 0.10 +0.8 627,628 + AO353_02950 0.10 +1.3 627,684 + AO353_02950 0.21 -0.1 627,684 + AO353_02950 0.21 -1.0 627,684 + AO353_02950 0.21 -0.1 627,684 + AO353_02950 0.21 -0.7 627,684 + AO353_02950 0.21 -0.5 627,684 + AO353_02950 0.21 +1.7 627,685 - AO353_02950 0.21 +1.4 627,685 - AO353_02950 0.21 +2.2 627,685 - AO353_02950 0.21 +1.4 627,685 - AO353_02950 0.21 -1.1 627,685 - AO353_02950 0.21 -0.1 627,735 + AO353_02950 0.31 -0.1 627,735 + AO353_02950 0.31 -1.4 627,735 + AO353_02950 0.31 +0.6 627,735 + AO353_02950 0.31 -0.3 627,735 + AO353_02950 0.31 +1.5 627,735 + AO353_02950 0.31 +2.4 627,735 + AO353_02950 0.31 +0.6 627,736 - AO353_02950 0.31 +0.5 627,736 - AO353_02950 0.31 -1.3 627,736 - AO353_02950 0.31 +0.9 627,736 - AO353_02950 0.31 -2.8 627,766 + AO353_02950 0.37 -0.5 627,766 + AO353_02950 0.37 -0.7 627,766 + AO353_02950 0.37 -0.3 627,767 - AO353_02950 0.37 +0.7 627,767 - AO353_02950 0.37 -0.7 627,767 - AO353_02950 0.37 +0.8 627,803 + AO353_02950 0.44 -1.7 627,803 + AO353_02950 0.44 -0.1 627,804 - AO353_02950 0.44 +1.8 627,804 - AO353_02950 0.44 -2.1 627,950 + AO353_02950 0.73 -0.1 627,950 + AO353_02950 0.73 +0.4 627,950 + AO353_02950 0.73 -0.4 627,951 - AO353_02950 0.73 -0.1 627,951 - AO353_02950 0.73 +0.1 627,951 - AO353_02950 0.73 -1.4 627,990 + AO353_02950 0.81 +0.8 627,990 + AO353_02950 0.81 +0.2 627,991 - AO353_02950 0.81 -0.4 627,991 - AO353_02950 0.81 -0.1 627,997 - AO353_02950 0.82 +0.1 628,051 + +0.9 628,052 - -1.7 628,052 - +0.1 628,052 - -0.4 628,052 - +0.9 628,052 - -0.1 628,052 - +1.8 628,159 - AO353_02955 0.66 -0.7 628,159 - AO353_02955 0.66 -1.4 628,229 - -0.4 628,229 - +0.9 628,229 - -0.7 628,229 - -0.2 628,229 - +0.1 628,238 - -1.1 628,254 - -2.1
Or see this region's nucleotide sequence