Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_619 and Pf1N1B4_620 are separated by 457 nucleotides Pf1N1B4_620 and Pf1N1B4_621 are separated by 11 nucleotides Pf1N1B4_621 and Pf1N1B4_622 are separated by 106 nucleotides
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Pf1N1B4_620 - Glycine cleavage system H protein, at 717,231 to 717,614
_620
Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478
_621
Pf1N1B4_622 - L-serine dehydratase (EC 4.3.1.17), at 720,585 to 721,961
_622
Position (kb) 717
718
719
720
721 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 716.670 kb on - strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.750 kb on + strand at 716.751 kb on - strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.843 kb on - strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.847 kb on - strand at 716.858 kb on + strand at 716.859 kb on - strand at 716.871 kb on + strand at 716.871 kb on + strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 717.072 kb on - strand at 717.221 kb on - strand at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.580 kb on - strand at 717.637 kb on + strand at 717.637 kb on + strand at 717.638 kb on - strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.885 kb on - strand at 717.898 kb on + strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.954 kb on - strand, within Pf1N1B4_621 at 717.954 kb on - strand, within Pf1N1B4_621 at 717.963 kb on - strand, within Pf1N1B4_621 at 718.475 kb on + strand at 718.475 kb on + strand, within Pf1N1B4_621 at 718.592 kb on - strand, within Pf1N1B4_621 at 718.635 kb on + strand, within Pf1N1B4_621 at 718.635 kb on + strand at 718.635 kb on + strand, within Pf1N1B4_621 at 718.636 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.799 kb on - strand, within Pf1N1B4_621 at 718.941 kb on + strand at 719.084 kb on + strand, within Pf1N1B4_621 at 719.369 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand at 719.648 kb on + strand at 719.648 kb on + strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.904 kb on - strand at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.912 kb on - strand, within Pf1N1B4_621 at 719.912 kb on - strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.970 kb on - strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.341 kb on + strand at 720.342 kb on - strand at 720.369 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.470 kb on + strand at 720.470 kb on + strand at 720.470 kb on + strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.548 kb on - strand at 720.548 kb on - strand at 720.548 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.565 kb on + strand at 720.565 kb on + strand at 720.566 kb on - strand at 720.566 kb on - strand at 720.566 kb on - strand at 720.820 kb on + strand, within Pf1N1B4_622 at 720.821 kb on - strand, within Pf1N1B4_622 at 720.948 kb on + strand, within Pf1N1B4_622 at 721.015 kb on - strand, within Pf1N1B4_622 at 721.017 kb on + strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.143 kb on + strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 716,670 - -0.4 716,746 + -0.0 716,746 + -0.0 716,746 + -1.6 716,747 - +0.7 716,747 - +0.2 716,747 - +0.6 716,750 + +1.2 716,751 - +0.2 716,757 + +0.6 716,757 + +0.4 716,757 + -0.0 716,758 - -0.6 716,758 - -0.3 716,758 - +1.3 716,761 + +2.6 716,761 + +1.3 716,761 + -0.4 716,762 - +1.0 716,762 - +0.2 716,762 - +0.2 716,762 - +0.8 716,762 - +0.5 716,762 - -0.8 716,843 - -0.0 716,846 + -1.6 716,846 + +1.2 716,846 + -2.6 716,847 - +1.6 716,858 + -0.2 716,859 - +0.7 716,871 + -1.0 716,871 + -0.4 716,872 - -0.0 716,872 - -0.3 716,872 - -0.6 716,872 - -0.3 716,872 - -1.0 717,072 - -0.6 717,221 - +1.0 717,426 + Pf1N1B4_620 0.51 +0.6 717,426 + Pf1N1B4_620 0.51 -0.8 717,426 + Pf1N1B4_620 0.51 +1.0 717,426 + Pf1N1B4_620 0.51 -0.8 717,427 - Pf1N1B4_620 0.51 +0.3 717,427 - Pf1N1B4_620 0.51 -0.0 717,427 - Pf1N1B4_620 0.51 -2.0 717,427 - +0.6 717,427 - Pf1N1B4_620 0.51 -0.0 717,427 - Pf1N1B4_620 0.51 +1.3 717,427 - Pf1N1B4_620 0.51 -0.0 717,427 - -1.0 717,580 - -1.0 717,637 + +2.6 717,637 + +0.4 717,638 - +1.0 717,869 + -1.0 717,869 + +1.0 717,869 + +2.0 717,870 - +0.5 717,870 - -0.3 717,870 - +0.5 717,885 - -0.4 717,898 + -0.0 717,899 - -0.6 717,899 - +1.8 717,899 - +1.1 717,899 - +1.0 717,954 - Pf1N1B4_621 0.11 +1.0 717,954 - Pf1N1B4_621 0.11 -0.0 717,963 - Pf1N1B4_621 0.12 +0.6 718,475 + +0.7 718,475 + Pf1N1B4_621 0.30 +1.2 718,592 - Pf1N1B4_621 0.34 -1.0 718,635 + Pf1N1B4_621 0.35 -0.4 718,635 + -0.6 718,635 + Pf1N1B4_621 0.35 -0.0 718,636 - Pf1N1B4_621 0.35 -0.0 718,662 - Pf1N1B4_621 0.36 +0.3 718,662 - Pf1N1B4_621 0.36 -1.0 718,662 - Pf1N1B4_621 0.36 +0.3 718,662 - Pf1N1B4_621 0.36 +0.3 718,662 - Pf1N1B4_621 0.36 +0.7 718,662 - Pf1N1B4_621 0.36 -0.6 718,799 - Pf1N1B4_621 0.41 -0.6 718,941 + -1.0 719,084 + Pf1N1B4_621 0.51 -1.0 719,369 + Pf1N1B4_621 0.61 +0.2 719,648 + Pf1N1B4_621 0.71 -0.0 719,648 + Pf1N1B4_621 0.71 -0.0 719,648 + -0.0 719,648 + -1.6 719,648 + Pf1N1B4_621 0.71 -0.1 719,649 - Pf1N1B4_621 0.71 -0.0 719,649 - Pf1N1B4_621 0.71 -0.4 719,649 - Pf1N1B4_621 0.71 -0.7 719,649 - Pf1N1B4_621 0.71 -0.5 719,649 - Pf1N1B4_621 0.71 -0.0 719,903 + Pf1N1B4_621 0.80 -0.3 719,903 + Pf1N1B4_621 0.80 -0.7 719,903 + Pf1N1B4_621 0.80 -1.0 719,903 + -1.0 719,903 + Pf1N1B4_621 0.80 +1.1 719,903 + Pf1N1B4_621 0.80 -1.6 719,903 + Pf1N1B4_621 0.80 -0.1 719,903 + Pf1N1B4_621 0.80 +1.0 719,903 + Pf1N1B4_621 0.80 +2.8 719,903 + Pf1N1B4_621 0.80 +0.6 719,904 - +1.6 719,904 - Pf1N1B4_621 0.80 -0.6 719,904 - Pf1N1B4_621 0.80 -0.4 719,904 - +0.6 719,904 - Pf1N1B4_621 0.80 -0.0 719,904 - Pf1N1B4_621 0.80 +2.6 719,911 + Pf1N1B4_621 0.80 -0.3 719,911 + Pf1N1B4_621 0.80 +0.3 719,911 + Pf1N1B4_621 0.80 -1.3 719,911 + Pf1N1B4_621 0.80 +1.1 719,911 + Pf1N1B4_621 0.80 -2.0 719,912 - Pf1N1B4_621 0.80 -0.0 719,912 - Pf1N1B4_621 0.80 -0.2 719,969 + Pf1N1B4_621 0.82 -1.0 719,969 + Pf1N1B4_621 0.82 -0.0 719,969 + Pf1N1B4_621 0.82 +0.2 719,969 + Pf1N1B4_621 0.82 -0.4 719,969 + Pf1N1B4_621 0.82 -1.0 719,970 - Pf1N1B4_621 0.82 -0.0 720,008 + Pf1N1B4_621 0.83 +1.0 720,008 + Pf1N1B4_621 0.83 -0.0 720,008 + Pf1N1B4_621 0.83 -0.0 720,008 + Pf1N1B4_621 0.83 -0.0 720,009 - Pf1N1B4_621 0.84 +1.8 720,009 - Pf1N1B4_621 0.84 +1.0 720,009 - Pf1N1B4_621 0.84 -1.6 720,009 - Pf1N1B4_621 0.84 +0.1 720,009 - Pf1N1B4_621 0.84 -0.3 720,341 + -0.6 720,342 - -2.0 720,369 + +1.6 720,392 + +0.6 720,392 + +2.3 720,392 + -0.1 720,392 + +0.3 720,470 + +1.3 720,470 + -0.6 720,470 + +2.0 720,471 - +2.6 720,471 - +2.6 720,471 - -0.5 720,471 - -0.3 720,547 + +0.3 720,547 + -0.0 720,547 + -0.0 720,547 + -1.3 720,547 + +0.7 720,548 - -1.6 720,548 - -1.0 720,548 - +0.6 720,560 - -0.0 720,560 - -1.6 720,560 - -1.6 720,560 - -0.0 720,560 - -0.5 720,565 + +0.8 720,565 + +0.5 720,566 - +0.3 720,566 - -0.6 720,566 - -0.0 720,820 + Pf1N1B4_622 0.17 -0.0 720,821 - Pf1N1B4_622 0.17 -1.4 720,948 + Pf1N1B4_622 0.26 -0.0 721,015 - Pf1N1B4_622 0.31 +0.8 721,017 + Pf1N1B4_622 0.31 +0.6 721,018 - Pf1N1B4_622 0.31 -0.8 721,018 - Pf1N1B4_622 0.31 -0.0 721,143 + Pf1N1B4_622 0.41 -1.6 721,144 - Pf1N1B4_622 0.41 -0.3 721,144 - Pf1N1B4_622 0.41 +0.7 721,144 - Pf1N1B4_622 0.41 -2.0 721,144 - Pf1N1B4_622 0.41 +0.4 721,396 - Pf1N1B4_622 0.59 +0.7 721,396 - Pf1N1B4_622 0.59 -0.3
Or see this region's nucleotide sequence