Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3424 and Pf1N1B4_3425 are separated by 253 nucleotides Pf1N1B4_3425 and Pf1N1B4_3426 overlap by 4 nucleotides
Pf1N1B4_3424 - FIG00957532: hypothetical protein, at 3,814,024 to 3,815,013
_3424
Pf1N1B4_3425 - Glycerate kinase (EC 2.7.1.31), at 3,815,267 to 3,816,826
_3425
Pf1N1B4_3426 - McrBC 5-methylcytosine restriction system component, at 3,816,823 to 3,818,124
_3426
Position (kb)
3815
3816
3817 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3814.350 kb on - strand, within Pf1N1B4_3424 at 3814.350 kb on - strand, within Pf1N1B4_3424 at 3814.350 kb on - strand, within Pf1N1B4_3424 at 3814.350 kb on - strand, within Pf1N1B4_3424 at 3814.380 kb on - strand, within Pf1N1B4_3424 at 3814.644 kb on + strand, within Pf1N1B4_3424 at 3814.644 kb on + strand, within Pf1N1B4_3424 at 3814.652 kb on + strand, within Pf1N1B4_3424 at 3814.652 kb on + strand, within Pf1N1B4_3424 at 3814.652 kb on + strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.653 kb on - strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.760 kb on + strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.761 kb on - strand, within Pf1N1B4_3424 at 3814.820 kb on + strand, within Pf1N1B4_3424 at 3814.821 kb on - strand, within Pf1N1B4_3424 at 3814.821 kb on - strand, within Pf1N1B4_3424 at 3814.821 kb on - strand, within Pf1N1B4_3424 at 3814.821 kb on - strand, within Pf1N1B4_3424 at 3814.821 kb on - strand, within Pf1N1B4_3424 at 3814.962 kb on - strand at 3814.962 kb on - strand at 3814.962 kb on - strand at 3815.046 kb on - strand at 3815.093 kb on + strand at 3815.094 kb on - strand at 3815.094 kb on - strand at 3815.094 kb on - strand at 3815.094 kb on - strand at 3815.094 kb on - strand at 3815.128 kb on + strand at 3815.128 kb on + strand at 3815.128 kb on + strand at 3815.129 kb on - strand at 3815.129 kb on - strand at 3815.129 kb on - strand at 3815.129 kb on - strand at 3815.140 kb on + strand at 3815.141 kb on - strand at 3815.168 kb on - strand at 3815.175 kb on - strand at 3815.325 kb on - strand at 3815.325 kb on - strand at 3815.601 kb on + strand, within Pf1N1B4_3425 at 3815.602 kb on - strand, within Pf1N1B4_3425 at 3815.606 kb on - strand, within Pf1N1B4_3425 at 3815.633 kb on + strand, within Pf1N1B4_3425 at 3815.637 kb on + strand, within Pf1N1B4_3425 at 3815.638 kb on - strand, within Pf1N1B4_3425 at 3815.710 kb on + strand, within Pf1N1B4_3425 at 3815.711 kb on - strand, within Pf1N1B4_3425 at 3815.732 kb on + strand, within Pf1N1B4_3425 at 3815.803 kb on + strand, within Pf1N1B4_3425 at 3815.816 kb on + strand, within Pf1N1B4_3425 at 3815.839 kb on + strand, within Pf1N1B4_3425 at 3815.929 kb on - strand, within Pf1N1B4_3425 at 3815.946 kb on + strand, within Pf1N1B4_3425 at 3816.083 kb on + strand, within Pf1N1B4_3425 at 3816.084 kb on - strand, within Pf1N1B4_3425 at 3816.129 kb on + strand, within Pf1N1B4_3425 at 3816.130 kb on - strand, within Pf1N1B4_3425 at 3816.234 kb on + strand, within Pf1N1B4_3425 at 3816.588 kb on + strand, within Pf1N1B4_3425 at 3817.012 kb on + strand, within Pf1N1B4_3426 at 3817.271 kb on + strand, within Pf1N1B4_3426 at 3817.292 kb on + strand at 3817.292 kb on + strand at 3817.293 kb on - strand, within Pf1N1B4_3426 at 3817.372 kb on + strand at 3817.372 kb on + strand, within Pf1N1B4_3426 at 3817.373 kb on - strand, within Pf1N1B4_3426 at 3817.449 kb on + strand, within Pf1N1B4_3426 at 3817.449 kb on + strand, within Pf1N1B4_3426 at 3817.449 kb on + strand, within Pf1N1B4_3426 at 3817.558 kb on + strand, within Pf1N1B4_3426 at 3817.558 kb on + strand, within Pf1N1B4_3426 at 3817.558 kb on + strand at 3817.558 kb on + strand, within Pf1N1B4_3426 at 3817.559 kb on - strand, within Pf1N1B4_3426 at 3817.559 kb on - strand, within Pf1N1B4_3426 at 3817.676 kb on - strand, within Pf1N1B4_3426 at 3817.679 kb on + strand, within Pf1N1B4_3426 at 3817.679 kb on + strand, within Pf1N1B4_3426 at 3817.679 kb on + strand, within Pf1N1B4_3426 at 3817.680 kb on - strand at 3817.744 kb on + strand, within Pf1N1B4_3426 at 3817.745 kb on - strand, within Pf1N1B4_3426 at 3817.812 kb on + strand, within Pf1N1B4_3426 at 3817.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,814,350 - Pf1N1B4_3424 0.33 -0.1 3,814,350 - Pf1N1B4_3424 0.33 +0.5 3,814,350 - Pf1N1B4_3424 0.33 +2.2 3,814,350 - Pf1N1B4_3424 0.33 +0.3 3,814,380 - Pf1N1B4_3424 0.36 -0.3 3,814,644 + Pf1N1B4_3424 0.63 +0.6 3,814,644 + Pf1N1B4_3424 0.63 -1.1 3,814,652 + Pf1N1B4_3424 0.63 -0.1 3,814,652 + Pf1N1B4_3424 0.63 -0.5 3,814,652 + Pf1N1B4_3424 0.63 +1.2 3,814,653 - Pf1N1B4_3424 0.64 +1.1 3,814,653 - Pf1N1B4_3424 0.64 +0.2 3,814,653 - Pf1N1B4_3424 0.64 -1.4 3,814,653 - Pf1N1B4_3424 0.64 +0.9 3,814,653 - Pf1N1B4_3424 0.64 +0.5 3,814,653 - Pf1N1B4_3424 0.64 -1.4 3,814,760 + Pf1N1B4_3424 0.74 -1.1 3,814,760 + Pf1N1B4_3424 0.74 +1.5 3,814,760 + Pf1N1B4_3424 0.74 -0.1 3,814,760 + Pf1N1B4_3424 0.74 +0.5 3,814,760 + Pf1N1B4_3424 0.74 +0.9 3,814,760 + Pf1N1B4_3424 0.74 +2.9 3,814,761 - Pf1N1B4_3424 0.74 +0.7 3,814,761 - Pf1N1B4_3424 0.74 -0.8 3,814,761 - Pf1N1B4_3424 0.74 -1.3 3,814,761 - Pf1N1B4_3424 0.74 -0.1 3,814,761 - Pf1N1B4_3424 0.74 -0.1 3,814,761 - Pf1N1B4_3424 0.74 -0.1 3,814,820 + Pf1N1B4_3424 0.80 -0.2 3,814,821 - Pf1N1B4_3424 0.81 +0.9 3,814,821 - Pf1N1B4_3424 0.81 -0.7 3,814,821 - Pf1N1B4_3424 0.81 +0.2 3,814,821 - Pf1N1B4_3424 0.81 -2.4 3,814,821 - Pf1N1B4_3424 0.81 +1.1 3,814,962 - +0.4 3,814,962 - -0.7 3,814,962 - +0.5 3,815,046 - -0.9 3,815,093 + -0.1 3,815,094 - +0.9 3,815,094 - -0.8 3,815,094 - -1.1 3,815,094 - -0.4 3,815,094 - +1.9 3,815,128 + -0.7 3,815,128 + +0.3 3,815,128 + +1.5 3,815,129 - +0.9 3,815,129 - +0.2 3,815,129 - +0.5 3,815,129 - -0.1 3,815,140 + +2.2 3,815,141 - -0.1 3,815,168 - -0.6 3,815,175 - -1.7 3,815,325 - -0.5 3,815,325 - +2.2 3,815,601 + Pf1N1B4_3425 0.21 +0.5 3,815,602 - Pf1N1B4_3425 0.21 -0.3 3,815,606 - Pf1N1B4_3425 0.22 -0.7 3,815,633 + Pf1N1B4_3425 0.23 -0.1 3,815,637 + Pf1N1B4_3425 0.24 -0.5 3,815,638 - Pf1N1B4_3425 0.24 -2.9 3,815,710 + Pf1N1B4_3425 0.28 -2.4 3,815,711 - Pf1N1B4_3425 0.28 +0.5 3,815,732 + Pf1N1B4_3425 0.30 -0.9 3,815,803 + Pf1N1B4_3425 0.34 -0.1 3,815,816 + Pf1N1B4_3425 0.35 -1.1 3,815,839 + Pf1N1B4_3425 0.37 -1.1 3,815,929 - Pf1N1B4_3425 0.42 +0.3 3,815,946 + Pf1N1B4_3425 0.44 -1.1 3,816,083 + Pf1N1B4_3425 0.52 -0.1 3,816,084 - Pf1N1B4_3425 0.52 +0.9 3,816,129 + Pf1N1B4_3425 0.55 +0.3 3,816,130 - Pf1N1B4_3425 0.55 -0.1 3,816,234 + Pf1N1B4_3425 0.62 -1.1 3,816,588 + Pf1N1B4_3425 0.85 +1.1 3,817,012 + Pf1N1B4_3426 0.15 -0.1 3,817,271 + Pf1N1B4_3426 0.34 -1.1 3,817,292 + -0.1 3,817,292 + -1.4 3,817,293 - Pf1N1B4_3426 0.36 -0.1 3,817,372 + +1.5 3,817,372 + Pf1N1B4_3426 0.42 -0.5 3,817,373 - Pf1N1B4_3426 0.42 +1.1 3,817,449 + Pf1N1B4_3426 0.48 -0.9 3,817,449 + Pf1N1B4_3426 0.48 -0.1 3,817,449 + Pf1N1B4_3426 0.48 -0.7 3,817,558 + Pf1N1B4_3426 0.56 +0.5 3,817,558 + Pf1N1B4_3426 0.56 +0.9 3,817,558 + -0.1 3,817,558 + Pf1N1B4_3426 0.56 +0.3 3,817,559 - Pf1N1B4_3426 0.57 +0.5 3,817,559 - Pf1N1B4_3426 0.57 -0.7 3,817,676 - Pf1N1B4_3426 0.66 +1.3 3,817,679 + Pf1N1B4_3426 0.66 +0.9 3,817,679 + Pf1N1B4_3426 0.66 +1.1 3,817,679 + Pf1N1B4_3426 0.66 +0.6 3,817,680 - -1.1 3,817,744 + Pf1N1B4_3426 0.71 +0.4 3,817,745 - Pf1N1B4_3426 0.71 +1.5 3,817,812 + Pf1N1B4_3426 0.76 +0.9 3,817,812 + +0.5
Or see this region's nucleotide sequence