Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3345 and Pf1N1B4_3346 overlap by 4 nucleotides Pf1N1B4_3346 and Pf1N1B4_3347 are separated by 120 nucleotides Pf1N1B4_3347 and Pf1N1B4_3348 are separated by 81 nucleotides Pf1N1B4_3348 and Pf1N1B4_3349 are separated by 277 nucleotides
Pf1N1B4_3345 - Ribonuclease D (EC 3.1.26.3), at 3,733,323 to 3,734,456
_3345
Pf1N1B4_3346 - Protein YcgL, at 3,734,453 to 3,734,746
_3346
Pf1N1B4_3347 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,734,867 to 3,735,799
_3347
Pf1N1B4_3348 - Nitroreductase family protein, at 3,735,881 to 3,736,474
_3348
Pf1N1B4_3349 - YcgN (Fragment), at 3,736,752 to 3,737,201
_3349
Position (kb)
3734
3735
3736 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3733.915 kb on - strand, within Pf1N1B4_3345 at 3733.915 kb on - strand, within Pf1N1B4_3345 at 3733.923 kb on + strand, within Pf1N1B4_3345 at 3733.924 kb on - strand, within Pf1N1B4_3345 at 3733.973 kb on - strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.475 kb on - strand at 3734.475 kb on - strand at 3734.475 kb on - strand at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.697 kb on - strand, within Pf1N1B4_3346 at 3734.697 kb on - strand, within Pf1N1B4_3346 at 3734.745 kb on - strand at 3734.822 kb on + strand at 3734.903 kb on + strand at 3734.903 kb on + strand at 3734.904 kb on - strand at 3734.904 kb on - strand at 3734.943 kb on + strand at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand at 3735.165 kb on - strand, within Pf1N1B4_3347 at 3735.165 kb on - strand, within Pf1N1B4_3347 at 3735.168 kb on + strand, within Pf1N1B4_3347 at 3735.168 kb on + strand, within Pf1N1B4_3347 at 3735.169 kb on - strand at 3735.169 kb on - strand, within Pf1N1B4_3347 at 3735.176 kb on + strand at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.183 kb on + strand, within Pf1N1B4_3347 at 3735.184 kb on - strand, within Pf1N1B4_3347 at 3735.348 kb on - strand, within Pf1N1B4_3347 at 3735.437 kb on + strand, within Pf1N1B4_3347 at 3735.437 kb on + strand, within Pf1N1B4_3347 at 3735.438 kb on - strand, within Pf1N1B4_3347 at 3735.438 kb on - strand, within Pf1N1B4_3347 at 3735.482 kb on + strand, within Pf1N1B4_3347 at 3736.302 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.447 kb on - strand at 3736.465 kb on + strand at 3736.489 kb on - strand at 3736.564 kb on + strand at 3736.564 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.646 kb on + strand at 3736.646 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.706 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.710 kb on - strand at 3736.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,733,915 - Pf1N1B4_3345 0.52 -0.4 3,733,915 - Pf1N1B4_3345 0.52 -0.1 3,733,923 + Pf1N1B4_3345 0.53 +1.0 3,733,924 - Pf1N1B4_3345 0.53 -1.1 3,733,973 - Pf1N1B4_3345 0.57 -0.1 3,734,107 + Pf1N1B4_3345 0.69 -0.3 3,734,107 + Pf1N1B4_3345 0.69 -1.6 3,734,107 + Pf1N1B4_3345 0.69 +0.9 3,734,107 + Pf1N1B4_3345 0.69 +0.2 3,734,108 - Pf1N1B4_3345 0.69 +0.5 3,734,108 - Pf1N1B4_3345 0.69 -0.9 3,734,108 - Pf1N1B4_3345 0.69 -0.3 3,734,108 - Pf1N1B4_3345 0.69 -0.4 3,734,108 - Pf1N1B4_3345 0.69 +0.9 3,734,462 - -0.1 3,734,462 - +0.2 3,734,462 - -0.1 3,734,462 - +0.9 3,734,462 - +1.1 3,734,475 - +0.3 3,734,475 - -1.1 3,734,475 - -0.1 3,734,502 - Pf1N1B4_3346 0.17 +0.9 3,734,502 - Pf1N1B4_3346 0.17 +0.8 3,734,502 - Pf1N1B4_3346 0.17 +0.3 3,734,502 - Pf1N1B4_3346 0.17 +0.3 3,734,502 - Pf1N1B4_3346 0.17 -0.1 3,734,502 - Pf1N1B4_3346 0.17 +1.1 3,734,507 + Pf1N1B4_3346 0.18 -0.1 3,734,507 + Pf1N1B4_3346 0.18 -0.1 3,734,507 + Pf1N1B4_3346 0.18 -0.3 3,734,508 - Pf1N1B4_3346 0.19 +1.2 3,734,508 - Pf1N1B4_3346 0.19 +1.5 3,734,508 - Pf1N1B4_3346 0.19 +1.1 3,734,508 - Pf1N1B4_3346 0.19 -0.1 3,734,508 - Pf1N1B4_3346 0.19 -0.4 3,734,508 - Pf1N1B4_3346 0.19 +0.7 3,734,508 - Pf1N1B4_3346 0.19 -0.1 3,734,508 - Pf1N1B4_3346 0.19 -0.1 3,734,508 - Pf1N1B4_3346 0.19 -0.1 3,734,508 - Pf1N1B4_3346 0.19 +0.6 3,734,508 - Pf1N1B4_3346 0.19 -0.1 3,734,697 - Pf1N1B4_3346 0.83 -0.6 3,734,697 - Pf1N1B4_3346 0.83 +0.3 3,734,745 - +0.9 3,734,822 + -0.5 3,734,903 + -0.7 3,734,903 + -0.1 3,734,904 - -0.1 3,734,904 - +0.9 3,734,943 + +1.9 3,735,164 + Pf1N1B4_3347 0.32 +0.5 3,735,164 + Pf1N1B4_3347 0.32 -0.1 3,735,164 + Pf1N1B4_3347 0.32 -0.4 3,735,164 + -1.1 3,735,165 - Pf1N1B4_3347 0.32 +1.5 3,735,165 - Pf1N1B4_3347 0.32 +0.9 3,735,168 + Pf1N1B4_3347 0.32 -0.1 3,735,168 + Pf1N1B4_3347 0.32 +1.5 3,735,169 - -0.7 3,735,169 - Pf1N1B4_3347 0.32 +1.2 3,735,176 + -1.1 3,735,176 + Pf1N1B4_3347 0.33 +2.4 3,735,176 + Pf1N1B4_3347 0.33 -2.1 3,735,176 + Pf1N1B4_3347 0.33 +0.9 3,735,176 + Pf1N1B4_3347 0.33 +0.3 3,735,176 + Pf1N1B4_3347 0.33 +0.7 3,735,176 + Pf1N1B4_3347 0.33 -0.7 3,735,176 + Pf1N1B4_3347 0.33 +0.7 3,735,176 + Pf1N1B4_3347 0.33 +0.5 3,735,176 + Pf1N1B4_3347 0.33 +0.7 3,735,176 + Pf1N1B4_3347 0.33 +0.8 3,735,177 - Pf1N1B4_3347 0.33 -0.1 3,735,177 - Pf1N1B4_3347 0.33 +0.9 3,735,177 - -0.1 3,735,177 - Pf1N1B4_3347 0.33 -2.4 3,735,177 - Pf1N1B4_3347 0.33 +0.3 3,735,177 - Pf1N1B4_3347 0.33 -1.7 3,735,177 - Pf1N1B4_3347 0.33 +2.2 3,735,177 - Pf1N1B4_3347 0.33 -1.1 3,735,177 - Pf1N1B4_3347 0.33 +0.1 3,735,177 - +0.9 3,735,177 - Pf1N1B4_3347 0.33 -0.1 3,735,177 - Pf1N1B4_3347 0.33 -1.1 3,735,177 - Pf1N1B4_3347 0.33 +0.2 3,735,183 + Pf1N1B4_3347 0.34 -0.3 3,735,184 - Pf1N1B4_3347 0.34 -1.1 3,735,348 - Pf1N1B4_3347 0.52 -0.1 3,735,437 + Pf1N1B4_3347 0.61 -0.1 3,735,437 + Pf1N1B4_3347 0.61 +0.2 3,735,438 - Pf1N1B4_3347 0.61 -0.4 3,735,438 - Pf1N1B4_3347 0.61 +1.9 3,735,482 + Pf1N1B4_3347 0.66 -0.7 3,736,302 + Pf1N1B4_3348 0.71 -1.4 3,736,329 + Pf1N1B4_3348 0.75 -0.1 3,736,329 + Pf1N1B4_3348 0.75 -1.1 3,736,447 - +0.3 3,736,465 + +1.5 3,736,489 - -0.1 3,736,564 + -0.7 3,736,564 + -0.1 3,736,633 + -2.4 3,736,633 + -1.4 3,736,633 + +0.5 3,736,634 - +0.5 3,736,634 - -0.1 3,736,634 - +0.5 3,736,634 - +0.1 3,736,646 + -0.3 3,736,646 + +0.4 3,736,648 + -1.4 3,736,648 + +0.7 3,736,648 + -0.8 3,736,649 - +1.5 3,736,649 - -0.6 3,736,649 - -1.0 3,736,649 - -1.1 3,736,649 - +0.9 3,736,706 + -2.4 3,736,709 + +0.1 3,736,709 + -1.7 3,736,709 + +1.2 3,736,710 - +0.5 3,736,710 - -0.1
Or see this region's nucleotide sequence