Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2861 and Pf1N1B4_2862 are separated by 207 nucleotides Pf1N1B4_2862 and Pf1N1B4_2863 are separated by 334 nucleotides Pf1N1B4_2863 and Pf1N1B4_2864 overlap by 1 nucleotides Pf1N1B4_2864 and Pf1N1B4_2865 overlap by 4 nucleotides
Pf1N1B4_2861 - glutamyl-Q-tRNA synthetase, at 3,209,533 to 3,210,429
_2861
Pf1N1B4_2862 - C4-type zinc finger protein, DksA/TraR family, at 3,210,637 to 3,211,077
_2862
Pf1N1B4_2863 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 3,211,412 to 3,212,584
_2863
Pf1N1B4_2864 - Sugar/maltose fermentation stimulation protein homolog, at 3,212,584 to 3,213,297
_2864
Pf1N1B4_2865 - Rieske 2Fe-2S family protein, at 3,213,294 to 3,213,611
_2865
Position (kb)
3211
3212
3213 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3210.429 kb on + strand at 3210.429 kb on + strand at 3210.430 kb on - strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.513 kb on + strand at 3210.638 kb on + strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3211.102 kb on - strand at 3211.102 kb on - strand at 3211.102 kb on - strand at 3211.275 kb on + strand at 3211.275 kb on + strand at 3211.284 kb on + strand at 3211.285 kb on - strand at 3211.301 kb on + strand at 3211.301 kb on + strand at 3211.302 kb on - strand at 3211.302 kb on - strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.330 kb on + strand at 3211.330 kb on + strand at 3211.330 kb on + strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.348 kb on + strand at 3211.348 kb on + strand at 3211.348 kb on + strand at 3211.349 kb on - strand at 3211.376 kb on + strand at 3211.377 kb on - strand at 3211.400 kb on + strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.424 kb on + strand at 3211.425 kb on - strand at 3211.425 kb on - strand at 3211.425 kb on - strand at 3211.656 kb on - strand, within Pf1N1B4_2863 at 3211.759 kb on + strand, within Pf1N1B4_2863 at 3211.759 kb on + strand, within Pf1N1B4_2863 at 3211.759 kb on + strand, within Pf1N1B4_2863 at 3211.759 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.030 kb on + strand, within Pf1N1B4_2863 at 3212.031 kb on - strand, within Pf1N1B4_2863 at 3212.031 kb on - strand, within Pf1N1B4_2863 at 3212.031 kb on - strand, within Pf1N1B4_2863 at 3212.046 kb on - strand, within Pf1N1B4_2863 at 3212.102 kb on - strand, within Pf1N1B4_2863 at 3212.114 kb on + strand, within Pf1N1B4_2863 at 3212.114 kb on + strand, within Pf1N1B4_2863 at 3212.115 kb on - strand, within Pf1N1B4_2863 at 3212.151 kb on + strand, within Pf1N1B4_2863 at 3212.152 kb on - strand, within Pf1N1B4_2863 at 3212.152 kb on - strand, within Pf1N1B4_2863 at 3212.201 kb on + strand, within Pf1N1B4_2863 at 3212.202 kb on - strand, within Pf1N1B4_2863 at 3212.202 kb on - strand, within Pf1N1B4_2863 at 3212.202 kb on - strand, within Pf1N1B4_2863 at 3212.373 kb on - strand, within Pf1N1B4_2863 at 3212.373 kb on - strand, within Pf1N1B4_2863 at 3212.510 kb on + strand at 3212.629 kb on + strand at 3212.766 kb on + strand, within Pf1N1B4_2864 at 3212.920 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.977 kb on + strand at 3212.977 kb on + strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3212.978 kb on - strand, within Pf1N1B4_2864 at 3213.118 kb on + strand, within Pf1N1B4_2864 at 3213.118 kb on + strand, within Pf1N1B4_2864 at 3213.118 kb on + strand, within Pf1N1B4_2864 at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.119 kb on - strand at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.119 kb on - strand, within Pf1N1B4_2864 at 3213.229 kb on + strand at 3213.229 kb on + strand at 3213.230 kb on - strand at 3213.287 kb on + strand at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand, within Pf1N1B4_2865 at 3213.508 kb on + strand at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865 at 3213.509 kb on - strand, within Pf1N1B4_2865
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,210,429 + -2.4 3,210,429 + -1.4 3,210,430 - -0.4 3,210,441 + +2.6 3,210,441 + -0.9 3,210,441 + +0.1 3,210,441 + +0.1 3,210,441 + -0.2 3,210,441 + +1.1 3,210,441 + +0.5 3,210,441 + -2.2 3,210,441 + +0.1 3,210,441 + +0.7 3,210,441 + -0.0 3,210,441 + -0.2 3,210,442 - -0.9 3,210,442 - +1.1 3,210,442 - +0.3 3,210,442 - +1.7 3,210,513 + +1.7 3,210,638 + +0.1 3,210,639 - +0.9 3,210,639 - -1.2 3,210,639 - +1.1 3,210,639 - +1.5 3,210,639 - -0.4 3,211,102 - -2.2 3,211,102 - +1.0 3,211,102 - -0.0 3,211,275 + -0.9 3,211,275 + +0.1 3,211,284 + -1.4 3,211,285 - +0.7 3,211,301 + +1.1 3,211,301 + -1.2 3,211,302 - +0.5 3,211,302 - +1.5 3,211,328 + -0.9 3,211,328 + +2.0 3,211,328 + +0.1 3,211,328 + -0.3 3,211,329 - -0.4 3,211,329 - -0.6 3,211,329 - +0.6 3,211,329 - -0.6 3,211,329 - -0.4 3,211,329 - +0.1 3,211,329 - +0.4 3,211,329 - +0.7 3,211,329 - -0.4 3,211,329 - -0.6 3,211,329 - +2.5 3,211,330 + +0.9 3,211,330 + +0.7 3,211,330 + +0.4 3,211,331 - +1.1 3,211,331 - +1.1 3,211,331 - +0.7 3,211,331 - +0.1 3,211,348 + +0.6 3,211,348 + +0.3 3,211,348 + -1.2 3,211,349 - +0.7 3,211,376 + -0.4 3,211,377 - +0.4 3,211,400 + -0.9 3,211,401 - -0.9 3,211,401 - +0.1 3,211,401 - -1.7 3,211,401 - -1.2 3,211,401 - -1.9 3,211,401 - -0.3 3,211,401 - -0.3 3,211,424 + -3.3 3,211,425 - +1.1 3,211,425 - +2.1 3,211,425 - -2.4 3,211,656 - Pf1N1B4_2863 0.21 -0.3 3,211,759 + Pf1N1B4_2863 0.30 +1.1 3,211,759 + Pf1N1B4_2863 0.30 -0.9 3,211,759 + Pf1N1B4_2863 0.30 -0.3 3,211,759 + Pf1N1B4_2863 0.30 +0.1 3,212,030 + -1.9 3,212,030 + Pf1N1B4_2863 0.53 -0.1 3,212,030 + Pf1N1B4_2863 0.53 -0.9 3,212,030 + Pf1N1B4_2863 0.53 +1.7 3,212,030 + Pf1N1B4_2863 0.53 -0.9 3,212,030 + Pf1N1B4_2863 0.53 -0.7 3,212,030 + Pf1N1B4_2863 0.53 +0.1 3,212,030 + Pf1N1B4_2863 0.53 +1.1 3,212,031 - Pf1N1B4_2863 0.53 +0.8 3,212,031 - Pf1N1B4_2863 0.53 +1.4 3,212,031 - Pf1N1B4_2863 0.53 -1.4 3,212,046 - Pf1N1B4_2863 0.54 -0.2 3,212,102 - Pf1N1B4_2863 0.59 +0.6 3,212,114 + Pf1N1B4_2863 0.60 +0.8 3,212,114 + Pf1N1B4_2863 0.60 -2.7 3,212,115 - Pf1N1B4_2863 0.60 -0.6 3,212,151 + Pf1N1B4_2863 0.63 -0.7 3,212,152 - Pf1N1B4_2863 0.63 +1.1 3,212,152 - Pf1N1B4_2863 0.63 -1.9 3,212,201 + Pf1N1B4_2863 0.67 +2.1 3,212,202 - Pf1N1B4_2863 0.67 +0.9 3,212,202 - Pf1N1B4_2863 0.67 -0.3 3,212,202 - Pf1N1B4_2863 0.67 +0.7 3,212,373 - Pf1N1B4_2863 0.82 +1.1 3,212,373 - Pf1N1B4_2863 0.82 -0.5 3,212,510 + -0.4 3,212,629 + +0.8 3,212,766 + Pf1N1B4_2864 0.25 +0.7 3,212,920 + Pf1N1B4_2864 0.47 -0.3 3,212,977 + Pf1N1B4_2864 0.55 +0.4 3,212,977 + Pf1N1B4_2864 0.55 +1.5 3,212,977 + Pf1N1B4_2864 0.55 -0.9 3,212,977 + Pf1N1B4_2864 0.55 -0.3 3,212,977 + Pf1N1B4_2864 0.55 +0.7 3,212,977 + Pf1N1B4_2864 0.55 -2.0 3,212,977 + +0.1 3,212,977 + Pf1N1B4_2864 0.55 -1.0 3,212,977 + Pf1N1B4_2864 0.55 -1.4 3,212,977 + Pf1N1B4_2864 0.55 +0.1 3,212,977 + Pf1N1B4_2864 0.55 -0.4 3,212,977 + Pf1N1B4_2864 0.55 +0.1 3,212,977 + Pf1N1B4_2864 0.55 +2.5 3,212,977 + +0.1 3,212,977 + Pf1N1B4_2864 0.55 -0.2 3,212,978 - Pf1N1B4_2864 0.55 +0.1 3,212,978 - Pf1N1B4_2864 0.55 -0.4 3,212,978 - Pf1N1B4_2864 0.55 +0.1 3,212,978 - Pf1N1B4_2864 0.55 +0.5 3,212,978 - Pf1N1B4_2864 0.55 +0.1 3,212,978 - Pf1N1B4_2864 0.55 +0.6 3,212,978 - Pf1N1B4_2864 0.55 +0.8 3,212,978 - Pf1N1B4_2864 0.55 +0.1 3,213,118 + Pf1N1B4_2864 0.75 -0.1 3,213,118 + Pf1N1B4_2864 0.75 +1.1 3,213,118 + Pf1N1B4_2864 0.75 -0.2 3,213,119 - Pf1N1B4_2864 0.75 -0.5 3,213,119 - +1.5 3,213,119 - Pf1N1B4_2864 0.75 +0.1 3,213,119 - Pf1N1B4_2864 0.75 +0.7 3,213,119 - Pf1N1B4_2864 0.75 -1.4 3,213,119 - Pf1N1B4_2864 0.75 +0.1 3,213,119 - Pf1N1B4_2864 0.75 +0.1 3,213,229 + -1.2 3,213,229 + -0.6 3,213,230 - +0.9 3,213,287 + +0.4 3,213,508 + Pf1N1B4_2865 0.67 -0.3 3,213,508 + Pf1N1B4_2865 0.67 -0.4 3,213,508 + Pf1N1B4_2865 0.67 -0.4 3,213,508 + Pf1N1B4_2865 0.67 +0.9 3,213,508 + Pf1N1B4_2865 0.67 -2.4 3,213,508 + Pf1N1B4_2865 0.67 +1.4 3,213,508 + Pf1N1B4_2865 0.67 -0.9 3,213,508 + Pf1N1B4_2865 0.67 +0.7 3,213,508 + Pf1N1B4_2865 0.67 +1.1 3,213,508 + Pf1N1B4_2865 0.67 +0.1 3,213,508 + Pf1N1B4_2865 0.67 +0.9 3,213,508 + Pf1N1B4_2865 0.67 +0.1 3,213,508 + Pf1N1B4_2865 0.67 +2.0 3,213,508 + +1.7 3,213,509 - Pf1N1B4_2865 0.68 -2.0 3,213,509 - Pf1N1B4_2865 0.68 -0.4 3,213,509 - Pf1N1B4_2865 0.68 -0.6 3,213,509 - Pf1N1B4_2865 0.68 +1.3 3,213,509 - Pf1N1B4_2865 0.68 -1.4 3,213,509 - Pf1N1B4_2865 0.68 +0.1 3,213,509 - Pf1N1B4_2865 0.68 +0.6 3,213,509 - Pf1N1B4_2865 0.68 -0.6 3,213,509 - Pf1N1B4_2865 0.68 +0.5 3,213,509 - Pf1N1B4_2865 0.68 +1.7 3,213,509 - Pf1N1B4_2865 0.68 +1.1 3,213,509 - Pf1N1B4_2865 0.68 +0.7
Or see this region's nucleotide sequence