Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_28

Experiment: D-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_27 and Pf1N1B4_28 are separated by 235 nucleotidesPf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotidesPf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotidesPf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotides Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749 _27 Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185 _28 Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412 _29 Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710 _5164 Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899 _30 Position (kb) 31 32 33Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 31.006 kb on + strand, within Pf1N1B4_27at 31.007 kb on - strandat 31.405 kb on + strand, within Pf1N1B4_27at 31.405 kb on + strand, within Pf1N1B4_27at 31.405 kb on + strand, within Pf1N1B4_27at 31.405 kb on + strand, within Pf1N1B4_27at 31.406 kb on - strand, within Pf1N1B4_27at 31.606 kb on + strand, within Pf1N1B4_27at 31.631 kb on + strandat 31.631 kb on + strandat 31.660 kb on + strandat 31.660 kb on + strandat 31.660 kb on + strandat 31.660 kb on + strandat 31.660 kb on + strandat 31.661 kb on - strandat 31.661 kb on - strandat 31.661 kb on - strandat 31.661 kb on - strandat 31.762 kb on + strandat 31.781 kb on - strandat 31.797 kb on + strandat 31.798 kb on - strandat 31.810 kb on + strandat 31.892 kb on - strandat 31.952 kb on + strandat 31.952 kb on + strandat 31.958 kb on + strandat 31.958 kb on + strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 32.001 kb on + strandat 32.002 kb on - strandat 32.144 kb on + strand, within Pf1N1B4_28at 32.170 kb on + strandat 32.171 kb on - strandat 32.171 kb on - strandat 32.223 kb on - strand, within Pf1N1B4_29at 32.223 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.463 kb on + strandat 32.517 kb on + strandat 32.526 kb on - strandat 32.811 kb on + strandat 32.844 kb on + strandat 32.845 kb on - strandat 32.880 kb on + strandat 32.880 kb on + strandat 32.880 kb on + strandat 32.881 kb on - strandat 32.881 kb on - strandat 32.881 kb on - strandat 32.943 kb on + strandat 32.944 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (C)
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31,006 + Pf1N1B4_27 0.38 -2.6
31,007 - -0.2
31,405 + Pf1N1B4_27 0.71 -2.5
31,405 + Pf1N1B4_27 0.71 -0.0
31,405 + Pf1N1B4_27 0.71 -2.2
31,405 + Pf1N1B4_27 0.71 -2.6
31,406 - Pf1N1B4_27 0.71 -0.2
31,606 + Pf1N1B4_27 0.88 -1.2
31,631 + -2.6
31,631 + -0.2
31,660 + -1.2
31,660 + +0.8
31,660 + -2.2
31,660 + -1.7
31,660 + -2.6
31,661 - -2.2
31,661 - -0.2
31,661 - -3.0
31,661 - -0.2
31,762 + -0.6
31,781 - +0.3
31,797 + -0.1
31,798 - -0.2
31,810 + +0.8
31,892 - -1.2
31,952 + -0.2
31,952 + +1.3
31,958 + +0.3
31,958 + -0.1
31,959 - -0.2
31,959 - +1.1
31,959 - -0.4
31,959 - +0.8
31,959 - -0.2
32,001 + +0.2
32,002 - -0.2
32,144 + Pf1N1B4_28 0.79 +0.3
32,170 + -0.2
32,171 - -0.5
32,171 - +0.8
32,223 - Pf1N1B4_29 0.18 -0.6
32,223 - Pf1N1B4_29 0.18 -0.1
32,232 - Pf1N1B4_29 0.22 +0.1
32,232 - Pf1N1B4_29 0.22 -0.6
32,232 - Pf1N1B4_29 0.22 +0.8
32,463 + +1.8
32,517 + +1.3
32,526 - -0.2
32,811 + -0.2
32,844 + -0.2
32,845 - -0.2
32,880 + +1.6
32,880 + +1.8
32,880 + -1.4
32,881 - -0.8
32,881 - -0.2
32,881 - -0.2
32,943 + -0.1
32,944 - +1.0

Or see this region's nucleotide sequence