Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2777 and Pf1N1B4_2778 are separated by 383 nucleotides Pf1N1B4_2778 and Pf1N1B4_2779 are separated by 253 nucleotides
Pf1N1B4_2777 - probable dicarboxylate transporter, at 3,117,702 to 3,119,015
_2777
Pf1N1B4_2778 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 3,119,399 to 3,120,652
_2778
Pf1N1B4_2779 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 3,120,906 to 3,124,727
_2779
Position (kb)
3119
3120
3121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3118.493 kb on + strand, within Pf1N1B4_2777 at 3118.493 kb on + strand, within Pf1N1B4_2777 at 3118.767 kb on + strand, within Pf1N1B4_2777 at 3118.767 kb on + strand, within Pf1N1B4_2777 at 3118.767 kb on + strand, within Pf1N1B4_2777 at 3118.767 kb on + strand, within Pf1N1B4_2777 at 3118.767 kb on + strand, within Pf1N1B4_2777 at 3118.777 kb on + strand, within Pf1N1B4_2777 at 3118.777 kb on + strand, within Pf1N1B4_2777 at 3118.777 kb on + strand, within Pf1N1B4_2777 at 3118.777 kb on + strand, within Pf1N1B4_2777 at 3118.777 kb on + strand, within Pf1N1B4_2777 at 3118.778 kb on - strand, within Pf1N1B4_2777 at 3118.927 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.075 kb on + strand at 3119.076 kb on - strand at 3119.079 kb on + strand at 3119.079 kb on + strand at 3119.079 kb on + strand at 3119.097 kb on - strand at 3119.118 kb on + strand at 3119.118 kb on + strand at 3119.277 kb on + strand at 3119.277 kb on + strand at 3119.277 kb on + strand at 3119.277 kb on + strand at 3119.277 kb on + strand at 3119.278 kb on - strand at 3119.278 kb on - strand at 3119.278 kb on - strand at 3119.278 kb on - strand at 3119.290 kb on + strand at 3119.291 kb on - strand at 3119.291 kb on - strand at 3119.332 kb on + strand at 3119.333 kb on - strand at 3119.333 kb on - strand at 3119.400 kb on + strand at 3119.400 kb on + strand at 3119.400 kb on + strand at 3119.401 kb on - strand at 3119.401 kb on - strand at 3119.401 kb on - strand at 3120.708 kb on - strand at 3120.708 kb on - strand at 3120.708 kb on - strand at 3120.716 kb on + strand at 3120.716 kb on + strand at 3120.716 kb on + strand at 3120.717 kb on - strand at 3120.717 kb on - strand at 3120.982 kb on + strand at 3120.983 kb on - strand at 3121.187 kb on - strand at 3121.187 kb on - strand at 3121.187 kb on - strand at 3121.187 kb on - strand at 3121.187 kb on - strand at 3121.628 kb on - strand at 3121.628 kb on - strand, within Pf1N1B4_2779 at 3121.630 kb on + strand, within Pf1N1B4_2779 at 3121.630 kb on + strand, within Pf1N1B4_2779 at 3121.631 kb on - strand, within Pf1N1B4_2779 at 3121.631 kb on - strand, within Pf1N1B4_2779 at 3121.631 kb on - strand, within Pf1N1B4_2779
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,118,493 + Pf1N1B4_2777 0.60 -1.4 3,118,493 + Pf1N1B4_2777 0.60 -1.4 3,118,767 + Pf1N1B4_2777 0.81 +0.2 3,118,767 + Pf1N1B4_2777 0.81 -0.8 3,118,767 + Pf1N1B4_2777 0.81 +0.2 3,118,767 + Pf1N1B4_2777 0.81 -0.4 3,118,767 + Pf1N1B4_2777 0.81 +0.6 3,118,777 + Pf1N1B4_2777 0.82 +0.9 3,118,777 + Pf1N1B4_2777 0.82 +0.2 3,118,777 + Pf1N1B4_2777 0.82 -0.4 3,118,777 + Pf1N1B4_2777 0.82 -0.4 3,118,777 + Pf1N1B4_2777 0.82 -1.2 3,118,778 - Pf1N1B4_2777 0.82 +0.5 3,118,927 + -0.4 3,119,075 + -0.8 3,119,075 + -1.8 3,119,075 + +0.1 3,119,075 + +0.2 3,119,075 + +0.3 3,119,075 + -1.1 3,119,075 + -0.3 3,119,076 - +0.2 3,119,079 + -0.9 3,119,079 + +1.2 3,119,079 + +0.2 3,119,097 - +0.2 3,119,118 + +1.0 3,119,118 + -0.7 3,119,277 + +0.2 3,119,277 + -0.9 3,119,277 + -1.6 3,119,277 + +0.8 3,119,277 + +0.0 3,119,278 - +0.9 3,119,278 - +1.4 3,119,278 - +1.2 3,119,278 - +0.2 3,119,290 + -0.6 3,119,291 - -0.8 3,119,291 - -0.1 3,119,332 + +0.2 3,119,333 - -1.4 3,119,333 - +0.7 3,119,400 + -3.0 3,119,400 + +0.6 3,119,400 + -0.2 3,119,401 - -0.4 3,119,401 - +0.7 3,119,401 - -0.4 3,120,708 - -1.4 3,120,708 - -0.8 3,120,708 - -2.8 3,120,716 + -1.8 3,120,716 + -1.4 3,120,716 + -1.4 3,120,717 - +0.6 3,120,717 - +0.2 3,120,982 + +1.8 3,120,983 - +0.2 3,121,187 - +0.8 3,121,187 - +0.4 3,121,187 - -0.4 3,121,187 - -0.4 3,121,187 - -0.7 3,121,628 - +2.8 3,121,628 - Pf1N1B4_2779 0.19 -0.2 3,121,630 + Pf1N1B4_2779 0.19 +0.5 3,121,630 + Pf1N1B4_2779 0.19 -0.4 3,121,631 - Pf1N1B4_2779 0.19 -0.4 3,121,631 - Pf1N1B4_2779 0.19 -0.4 3,121,631 - Pf1N1B4_2779 0.19 +1.8
Or see this region's nucleotide sequence