Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS20680

Experiment: Temperature shift 10_to_34; with MOPS

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 10_to_34; with MOPS
4,656,614	-		PFLU_RS20675	0.37	-0.7
4,656,717	+		PFLU_RS20675	0.44	+0.4
4,656,717	+		PFLU_RS20675	0.44	+2.4
4,656,717	+		PFLU_RS20675	0.44	+0.5
4,656,718	-		PFLU_RS20675	0.44	+0.2
4,656,718	-		PFLU_RS20675	0.44	-0.9
4,656,947	+		PFLU_RS20675	0.61	+1.1
4,656,947	+		PFLU_RS20675	0.61	+0.3
4,656,948	-		PFLU_RS20675	0.61	+1.5
4,656,948	-		PFLU_RS20675	0.61	-0.3
4,656,948	-		PFLU_RS20675	0.61	+0.0
4,656,948	-		PFLU_RS20675	0.61	+0.3
4,656,948	-		PFLU_RS20675	0.61	+0.4
4,656,948	-		PFLU_RS20675	0.61	-0.4
4,656,948	-		PFLU_RS20675	0.61	+2.2
4,656,948	-		PFLU_RS20675	0.61	+0.3
4,657,021	+		PFLU_RS20675	0.66	+0.8
4,657,021	+		PFLU_RS20675	0.66	-0.4
4,657,021	+		PFLU_RS20675	0.66	-0.6
4,657,021	+		PFLU_RS20675	0.66	-0.8
4,657,021	+		PFLU_RS20675	0.66	-1.4
4,657,021	+		PFLU_RS20675	0.66	+0.4
4,657,021	+		PFLU_RS20675	0.66	-0.2
4,657,021	+		PFLU_RS20675	0.66	+1.5
4,657,021	+		PFLU_RS20675	0.66	+0.1
4,657,021	+		PFLU_RS20675	0.66	+2.2
4,657,022	-		PFLU_RS20675	0.66	+0.8
4,657,022	-		PFLU_RS20675	0.66	-0.4
4,657,022	-		PFLU_RS20675	0.66	+1.1
4,657,022	-		PFLU_RS20675	0.66	+0.3
4,657,022	-		PFLU_RS20675	0.66	+0.3
4,657,022	-		PFLU_RS20675	0.66	+1.3
4,657,022	-		PFLU_RS20675	0.66	+1.6
4,657,022	-		PFLU_RS20675	0.66	+0.5
4,657,022	-		PFLU_RS20675	0.66	-0.7
4,657,022	-		PFLU_RS20675	0.66	-0.2
4,657,022	-		PFLU_RS20675	0.66	-0.4
4,657,022	-		PFLU_RS20675	0.66	-0.4
4,657,022	-		PFLU_RS20675	0.66	+0.1
4,657,141	+		PFLU_RS20675	0.75	-1.8
4,657,141	+		PFLU_RS20675	0.75	-0.9
4,657,141	+		PFLU_RS20675	0.75	+0.0
4,657,141	+		PFLU_RS20675	0.75	+0.4
4,657,141	+		PFLU_RS20675	0.75	-0.1
4,657,141	+		PFLU_RS20675	0.75	+0.9
4,657,142	-		PFLU_RS20675	0.75	+0.2
4,657,142	-		PFLU_RS20675	0.75	+0.1
4,657,142	-		PFLU_RS20675	0.75	+0.8
4,657,142	-		PFLU_RS20675	0.75	-1.2
4,657,142	-		PFLU_RS20675	0.75	+0.2
4,657,142	-		PFLU_RS20675	0.75	+0.1
4,657,142	-		PFLU_RS20675	0.75	-1.4
4,657,144	+		PFLU_RS20675	0.75	-0.4
4,657,144	+		PFLU_RS20675	0.75	-0.4
4,657,144	+		PFLU_RS20675	0.75	+0.9
4,657,144	+		PFLU_RS20675	0.75	-0.3
4,657,144	+		PFLU_RS20675	0.75	+0.0
4,657,144	+		PFLU_RS20675	0.75	+1.0
4,657,144	+		PFLU_RS20675	0.75	-1.1
4,657,144	+		PFLU_RS20675	0.75	+0.3
4,657,144	+		PFLU_RS20675	0.75	-0.2
4,657,145	-		PFLU_RS20675	0.75	+0.8
4,657,145	-		PFLU_RS20675	0.75	+1.3
4,657,157	+		PFLU_RS20675	0.76	-0.5
4,657,158	-		PFLU_RS20675	0.76	+0.9
4,657,158	-		PFLU_RS20675	0.76	+0.1
4,657,492	+				-1.0
4,657,492	+				+0.2
4,657,492	+				-0.1
4,657,492	+				-0.1
4,657,492	+				-0.2
4,657,493	-				-1.4
4,657,493	-				-1.4
4,657,493	-				+1.0
4,657,493	-				-0.6
4,657,551	+				-0.8
4,657,552	-				+0.1
4,657,560	+				-0.4
4,657,560	+				+1.2
4,657,560	+				-1.6
4,657,560	+				+0.1
4,657,561	-				+0.6
4,657,561	-				-1.1
4,657,575	-				+0.1
4,657,598	+				-0.7
4,657,598	+				-0.8
4,657,598	+				+1.5
4,657,599	-				-2.4
4,657,599	-				+0.0
4,657,658	-				+0.1
4,657,658	-				-0.6
4,657,931	+		PFLU_RS20680	0.36	-0.2
4,657,994	+		PFLU_RS20680	0.43	-0.2
4,657,995	-		PFLU_RS20680	0.43	+0.4
4,657,995	-		PFLU_RS20680	0.43	+0.9
4,657,995	-		PFLU_RS20680	0.43	+0.2
4,657,995	-		PFLU_RS20680	0.43	-1.0
4,658,009	+		PFLU_RS20680	0.45	-1.0
4,658,009	+		PFLU_RS20680	0.45	-0.8
4,658,009	+		PFLU_RS20680	0.45	-1.4
4,658,009	+		PFLU_RS20680	0.45	-0.4
4,658,009	+		PFLU_RS20680	0.45	-2.2
4,658,009	+		PFLU_RS20680	0.45	-0.2
4,658,009	+		PFLU_RS20680	0.45	-1.4
4,658,009	+		PFLU_RS20680	0.45	-2.6
4,658,009	+		PFLU_RS20680	0.45	-0.2
4,658,010	-		PFLU_RS20680	0.45	+0.5
4,658,010	-		PFLU_RS20680	0.45	-0.1
4,658,010	-		PFLU_RS20680	0.45	+0.9
4,658,010	-		PFLU_RS20680	0.45	+0.3
4,658,010	-		PFLU_RS20680	0.45	+2.2
4,658,010	-		PFLU_RS20680	0.45	-0.5
4,658,010	-		PFLU_RS20680	0.45	-0.0
4,658,010	-		PFLU_RS20680	0.45	+0.4
4,658,010	-		PFLU_RS20680	0.45	-0.5
4,658,010	-		PFLU_RS20680	0.45	+0.9
4,658,010	-		PFLU_RS20680	0.45	+0.6
4,658,010	-		PFLU_RS20680	0.45	+0.6
4,658,010	-		PFLU_RS20680	0.45	+1.9
4,658,010	-		PFLU_RS20680	0.45	+0.6
4,658,010	-		PFLU_RS20680	0.45	+2.2
4,658,010	-		PFLU_RS20680	0.45	+0.5
4,658,016	+		PFLU_RS20680	0.45	-1.9
4,658,016	+		PFLU_RS20680	0.45	+1.5
4,658,016	+		PFLU_RS20680	0.45	+0.8
4,658,016	+		PFLU_RS20680	0.45	+0.1
4,658,016	+		PFLU_RS20680	0.45	-1.6
4,658,016	+		PFLU_RS20680	0.45	+2.7
4,658,017	-		PFLU_RS20680	0.45	+0.0
4,658,017	-		PFLU_RS20680	0.45	+0.5
4,658,017	-		PFLU_RS20680	0.45	-0.6
4,658,017	-		PFLU_RS20680	0.45	-1.5
4,658,017	-		PFLU_RS20680	0.45	+0.8
4,658,017	-		PFLU_RS20680	0.45	+0.2
4,658,017	-		PFLU_RS20680	0.45	+0.3
4,658,017	-		PFLU_RS20680	0.45	+1.4
4,658,017	-		PFLU_RS20680	0.45	+1.8
4,658,079	+		PFLU_RS20680	0.52	+0.7
4,658,179	+		PFLU_RS20680	0.63	+0.9
4,658,179	+		PFLU_RS20680	0.63	-0.2
4,658,180	-		PFLU_RS20680	0.63	+0.9
4,658,180	-		PFLU_RS20680	0.63	+0.8
4,658,191	+		PFLU_RS20680	0.64	-0.2
4,658,191	+		PFLU_RS20680	0.64	+0.4
4,658,191	+		PFLU_RS20680	0.64	-1.1
4,658,191	+		PFLU_RS20680	0.64	-0.3
4,658,192	-		PFLU_RS20680	0.64	+0.6
4,658,251	-		PFLU_RS20680	0.71	+0.2
4,658,296	+		PFLU_RS20680	0.76	+0.1
4,658,297	-		PFLU_RS20680	0.76	-0.4
4,658,316	-		PFLU_RS20680	0.78	-0.2
4,658,409	+		PFLU_RS20680	0.88	-0.4
4,658,409	+		PFLU_RS20680	0.88	+1.1
4,658,409	+		PFLU_RS20680	0.88	+0.3
4,658,409	+		PFLU_RS20680	0.88	+2.2
4,658,409	+		PFLU_RS20680	0.88	+0.4
4,658,410	-		PFLU_RS20680	0.88	-0.8
4,658,410	-		PFLU_RS20680	0.88	+0.3
4,658,454	+				-1.0
4,658,595	+				-1.0
4,658,596	-				+0.1
4,658,596	-				+0.6
4,658,600	+				-0.7
4,658,600	+				-0.1
4,658,600	+				-1.1
4,658,601	-				+0.0
4,658,601	-				+1.5
4,658,654	+				-0.9
4,658,654	+				-0.4
4,658,676	+				-1.7
4,658,726	+				+2.1
4,658,738	+				-0.2
4,658,738	+				+0.1
4,658,738	+				-1.6
4,658,738	+				+0.0
4,658,738	+				+0.7
4,658,738	+				+0.3
4,658,738	+				+2.8
4,658,738	+				-1.4
4,658,739	-				+1.3
4,658,739	-				+0.8
4,658,739	-				-1.2
4,658,817	+		PFLU_RS20685	0.15	+0.0
4,658,817	+		PFLU_RS20685	0.15	+0.2
4,658,818	-		PFLU_RS20685	0.15	+2.0
4,658,818	-		PFLU_RS20685	0.15	+0.8
4,658,818	-		PFLU_RS20685	0.15	+0.2
4,658,818	-		PFLU_RS20685	0.15	-1.1
4,658,825	+		PFLU_RS20685	0.16	-0.4
4,658,825	+		PFLU_RS20685	0.16	-0.4
4,658,825	+		PFLU_RS20685	0.16	-1.1
4,658,825	+		PFLU_RS20685	0.16	-0.9
4,658,826	-		PFLU_RS20685	0.16	-0.3
4,658,826	-		PFLU_RS20685	0.16	+1.2
4,658,914	+		PFLU_RS20685	0.23	-1.2
4,658,915	-		PFLU_RS20685	0.23	-0.4
4,658,939	+		PFLU_RS20685	0.26	+1.2
4,658,939	+		PFLU_RS20685	0.26	-0.2
4,658,939	+		PFLU_RS20685	0.26	+1.1
4,658,939	+		PFLU_RS20685	0.26	-0.9
4,658,939	+		PFLU_RS20685	0.26	+0.6
4,658,939	+		PFLU_RS20685	0.26	-1.8
4,658,939	+		PFLU_RS20685	0.26	+0.1
4,658,940	-		PFLU_RS20685	0.26	-1.1
4,658,940	-		PFLU_RS20685	0.26	-0.4
4,658,940	-		PFLU_RS20685	0.26	-0.2
4,658,940	-		PFLU_RS20685	0.26	+0.1
4,658,940	-		PFLU_RS20685	0.26	-0.1
4,658,940	-		PFLU_RS20685	0.26	+3.5
4,658,940	-		PFLU_RS20685	0.26	-0.0
4,658,940	-		PFLU_RS20685	0.26	-0.8
4,658,940	-		PFLU_RS20685	0.26	+1.3
4,658,940	-		PFLU_RS20685	0.26	-0.8
4,658,940	-		PFLU_RS20685	0.26	+0.4
4,658,940	-		PFLU_RS20685	0.26	-1.6
4,658,940	-		PFLU_RS20685	0.26	-0.3
4,658,940	-		PFLU_RS20685	0.26	+0.9
4,658,970	-		PFLU_RS20685	0.28	+1.1
4,658,970	-		PFLU_RS20685	0.28	+0.1
4,658,970	-		PFLU_RS20685	0.28	+0.8
4,659,053	+		PFLU_RS20685	0.35	-0.8
4,659,053	+		PFLU_RS20685	0.35	-0.3
4,659,053	+		PFLU_RS20685	0.35	+0.1
4,659,053	+		PFLU_RS20685	0.35	+2.2
4,659,054	-		PFLU_RS20685	0.35	-0.5
4,659,054	-		PFLU_RS20685	0.35	+1.5
4,659,054	-		PFLU_RS20685	0.35	+0.7
4,659,054	-		PFLU_RS20685	0.35	+0.3
4,659,054	-		PFLU_RS20685	0.35	+0.7
4,659,054	-		PFLU_RS20685	0.35	-0.1
4,659,074	+		PFLU_RS20685	0.37	+0.4
4,659,075	-		PFLU_RS20685	0.37	-0.4
4,659,075	-		PFLU_RS20685	0.37	-1.0
4,659,174	-		PFLU_RS20685	0.45	-2.2
4,659,174	-		PFLU_RS20685	0.45	-1.1
4,659,174	-		PFLU_RS20685	0.45	-0.9
4,659,253	-		PFLU_RS20685	0.52	+0.4
4,659,253	-		PFLU_RS20685	0.52	-0.4
4,659,283	+		PFLU_RS20685	0.54	+0.0
4,659,283	+		PFLU_RS20685	0.54	-0.4
4,659,283	+		PFLU_RS20685	0.54	+2.8
4,659,283	+		PFLU_RS20685	0.54	+0.8
4,659,283	+		PFLU_RS20685	0.54	-1.9
4,659,283	+		PFLU_RS20685	0.54	+0.0
4,659,283	+		PFLU_RS20685	0.54	-1.1
4,659,283	+		PFLU_RS20685	0.54	-0.4
4,659,284	-		PFLU_RS20685	0.54	+0.3
4,659,284	-		PFLU_RS20685	0.54	+0.1
4,659,284	-		PFLU_RS20685	0.54	+0.1
4,659,284	-		PFLU_RS20685	0.54	+0.1
4,659,332	+		PFLU_RS20685	0.58	-0.6
4,659,333	-		PFLU_RS20685	0.58	+0.1
4,659,333	-		PFLU_RS20685	0.58	-1.8
4,659,333	-		PFLU_RS20685	0.58	+0.6
4,659,333	-		PFLU_RS20685	0.58	+1.2
4,659,344	+		PFLU_RS20685	0.59	-1.8
4,659,344	+		PFLU_RS20685	0.59	-0.4
4,659,345	-		PFLU_RS20685	0.59	-0.3
4,659,345	-		PFLU_RS20685	0.59	-1.1
4,659,434	+		PFLU_RS20685	0.67	+0.8
4,659,434	+		PFLU_RS20685	0.67	-0.9
4,659,434	+		PFLU_RS20685	0.67	-0.1
4,659,434	+		PFLU_RS20685	0.67	+0.9
4,659,435	-		PFLU_RS20685	0.67	+1.8
4,659,435	-		PFLU_RS20685	0.67	-0.1
4,659,435	-		PFLU_RS20685	0.67	+0.1