Experiment: Iron limitation (0.2x), formate media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06765 and MMJJ_RS06770 are separated by 116 nucleotides MMJJ_RS06770 and MMJJ_RS06775 are separated by 36 nucleotides MMJJ_RS06775 and MMJJ_RS06780 are separated by 39 nucleotides MMJJ_RS06780 and MMJJ_RS06785 are separated by 54 nucleotides
MMJJ_RS06765: MMJJ_RS06765 - hypothetical protein, at 1,249,512 to 1,250,249
_RS06765
MMJJ_RS06770: MMJJ_RS06770 - hypothetical protein, at 1,250,366 to 1,251,199
_RS06770
MMJJ_RS06775: MMJJ_RS06775 - isoleucine--tRNA ligase, at 1,251,236 to 1,254,340
_RS06775
MMJJ_RS06780: MMJJ_RS06780 - DUF356 domain-containing protein, at 1,254,380 to 1,254,760
_RS06780
MMJJ_RS06785: MMJJ_RS06785 - RNA ligase partner protein, at 1,254,815 to 1,255,486
_RS06785
Position (kb)
1251
1252
1253
1254
1255 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1250.240 kb on + strand at 1250.297 kb on - strand at 1250.297 kb on - strand at 1250.305 kb on - strand at 1250.306 kb on + strand at 1250.306 kb on + strand at 1250.328 kb on + strand at 1250.365 kb on - strand at 1250.365 kb on - strand at 1250.366 kb on + strand at 1250.366 kb on + strand at 1250.366 kb on + strand at 1250.412 kb on - strand at 1250.517 kb on - strand, within MMJJ_RS06770 at 1250.521 kb on - strand, within MMJJ_RS06770 at 1250.521 kb on - strand, within MMJJ_RS06770 at 1250.521 kb on - strand, within MMJJ_RS06770 at 1250.522 kb on + strand, within MMJJ_RS06770 at 1250.545 kb on + strand, within MMJJ_RS06770 at 1250.591 kb on - strand, within MMJJ_RS06770 at 1250.591 kb on - strand, within MMJJ_RS06770 at 1250.591 kb on - strand, within MMJJ_RS06770 at 1250.592 kb on + strand, within MMJJ_RS06770 at 1250.593 kb on - strand, within MMJJ_RS06770 at 1250.593 kb on - strand, within MMJJ_RS06770 at 1250.594 kb on + strand, within MMJJ_RS06770 at 1250.597 kb on + strand, within MMJJ_RS06770 at 1250.610 kb on - strand, within MMJJ_RS06770 at 1250.669 kb on + strand, within MMJJ_RS06770 at 1250.672 kb on - strand, within MMJJ_RS06770 at 1250.672 kb on - strand, within MMJJ_RS06770 at 1250.672 kb on - strand, within MMJJ_RS06770 at 1250.672 kb on - strand, within MMJJ_RS06770 at 1250.672 kb on - strand, within MMJJ_RS06770 at 1250.673 kb on + strand, within MMJJ_RS06770 at 1250.673 kb on + strand, within MMJJ_RS06770 at 1250.673 kb on + strand, within MMJJ_RS06770 at 1250.673 kb on + strand, within MMJJ_RS06770 at 1250.673 kb on + strand, within MMJJ_RS06770 at 1250.678 kb on - strand, within MMJJ_RS06770 at 1250.678 kb on - strand, within MMJJ_RS06770 at 1250.678 kb on - strand, within MMJJ_RS06770 at 1250.679 kb on + strand, within MMJJ_RS06770 at 1250.702 kb on + strand, within MMJJ_RS06770 at 1250.708 kb on - strand, within MMJJ_RS06770 at 1250.711 kb on - strand, within MMJJ_RS06770 at 1250.712 kb on + strand, within MMJJ_RS06770 at 1250.712 kb on + strand, within MMJJ_RS06770 at 1250.714 kb on - strand, within MMJJ_RS06770 at 1250.715 kb on + strand, within MMJJ_RS06770 at 1250.732 kb on - strand, within MMJJ_RS06770 at 1250.733 kb on + strand, within MMJJ_RS06770 at 1250.733 kb on + strand, within MMJJ_RS06770 at 1250.803 kb on - strand, within MMJJ_RS06770 at 1250.803 kb on - strand, within MMJJ_RS06770 at 1250.815 kb on + strand, within MMJJ_RS06770 at 1250.815 kb on + strand, within MMJJ_RS06770 at 1250.887 kb on + strand, within MMJJ_RS06770 at 1250.910 kb on - strand, within MMJJ_RS06770 at 1250.910 kb on - strand, within MMJJ_RS06770 at 1250.910 kb on - strand, within MMJJ_RS06770 at 1250.910 kb on - strand, within MMJJ_RS06770 at 1250.911 kb on + strand, within MMJJ_RS06770 at 1250.921 kb on + strand, within MMJJ_RS06770 at 1250.922 kb on - strand, within MMJJ_RS06770 at 1250.922 kb on - strand, within MMJJ_RS06770 at 1250.922 kb on - strand, within MMJJ_RS06770 at 1250.922 kb on - strand, within MMJJ_RS06770 at 1250.923 kb on + strand, within MMJJ_RS06770 at 1250.923 kb on + strand, within MMJJ_RS06770 at 1250.923 kb on + strand, within MMJJ_RS06770 at 1250.933 kb on - strand, within MMJJ_RS06770 at 1250.933 kb on - strand, within MMJJ_RS06770 at 1250.934 kb on + strand, within MMJJ_RS06770 at 1250.935 kb on - strand, within MMJJ_RS06770 at 1250.935 kb on - strand, within MMJJ_RS06770 at 1250.941 kb on - strand, within MMJJ_RS06770 at 1250.954 kb on - strand, within MMJJ_RS06770 at 1250.955 kb on + strand, within MMJJ_RS06770 at 1251.002 kb on + strand, within MMJJ_RS06770 at 1251.009 kb on + strand, within MMJJ_RS06770 at 1251.009 kb on + strand, within MMJJ_RS06770 at 1251.017 kb on + strand, within MMJJ_RS06770 at 1251.091 kb on - strand, within MMJJ_RS06770 at 1251.092 kb on + strand, within MMJJ_RS06770 at 1251.095 kb on - strand, within MMJJ_RS06770 at 1251.095 kb on - strand, within MMJJ_RS06770 at 1251.096 kb on + strand, within MMJJ_RS06770 at 1251.096 kb on + strand, within MMJJ_RS06770 at 1251.133 kb on - strand at 1251.135 kb on - strand at 1251.135 kb on - strand at 1251.135 kb on - strand at 1251.136 kb on + strand at 1251.170 kb on + strand at 1251.170 kb on + strand at 1251.170 kb on + strand at 1251.170 kb on + strand at 1251.179 kb on - strand at 1251.192 kb on + strand at 1251.203 kb on - strand at 1251.204 kb on + strand at 1251.206 kb on + strand at 1251.206 kb on + strand at 1251.214 kb on - strand at 1251.215 kb on + strand at 1251.237 kb on - strand at 1251.241 kb on - strand at 1252.006 kb on - strand at 1252.171 kb on + strand, within MMJJ_RS06775 at 1252.219 kb on + strand, within MMJJ_RS06775 at 1252.470 kb on - strand at 1253.831 kb on - strand, within MMJJ_RS06775 at 1253.930 kb on - strand, within MMJJ_RS06775 at 1254.422 kb on + strand, within MMJJ_RS06780 at 1254.506 kb on - strand, within MMJJ_RS06780 at 1254.799 kb on - strand at 1254.823 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.971 kb on - strand, within MMJJ_RS06785 at 1255.036 kb on - strand, within MMJJ_RS06785 at 1255.075 kb on - strand, within MMJJ_RS06785 at 1255.075 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.126 kb on + strand, within MMJJ_RS06785 at 1255.142 kb on - strand, within MMJJ_RS06785 at 1255.157 kb on + strand, within MMJJ_RS06785 at 1255.181 kb on - strand, within MMJJ_RS06785 at 1255.185 kb on - strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.191 kb on - strand, within MMJJ_RS06785 at 1255.191 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.215 kb on + strand, within MMJJ_RS06785 at 1255.222 kb on + strand, within MMJJ_RS06785 at 1255.315 kb on - strand, within MMJJ_RS06785 at 1255.315 kb on - strand, within MMJJ_RS06785
Per-strain Table
Position Strand Gene LocusTag Fraction Iron limitation (0.2x), formate media remove 1,250,240 + -0.4 1,250,297 - -0.2 1,250,297 - +0.4 1,250,305 - +1.2 1,250,306 + +0.1 1,250,306 + +0.8 1,250,328 + +0.8 1,250,365 - -3.1 1,250,365 - -0.2 1,250,366 + -0.2 1,250,366 + +0.1 1,250,366 + +0.2 1,250,412 - +0.4 1,250,517 - MMJJ_RS06770 0.18 +0.7 1,250,521 - MMJJ_RS06770 0.19 +0.5 1,250,521 - MMJJ_RS06770 0.19 -0.0 1,250,521 - MMJJ_RS06770 0.19 +1.0 1,250,522 + MMJJ_RS06770 0.19 -0.4 1,250,545 + MMJJ_RS06770 0.21 +0.8 1,250,591 - MMJJ_RS06770 0.27 +0.6 1,250,591 - MMJJ_RS06770 0.27 +0.4 1,250,591 - MMJJ_RS06770 0.27 +0.3 1,250,592 + MMJJ_RS06770 0.27 -0.2 1,250,593 - MMJJ_RS06770 0.27 -0.1 1,250,593 - MMJJ_RS06770 0.27 +0.1 1,250,594 + MMJJ_RS06770 0.27 +0.9 1,250,597 + MMJJ_RS06770 0.28 -0.5 1,250,610 - MMJJ_RS06770 0.29 -0.9 1,250,669 + MMJJ_RS06770 0.36 +0.0 1,250,672 - MMJJ_RS06770 0.37 +0.1 1,250,672 - MMJJ_RS06770 0.37 +0.1 1,250,672 - MMJJ_RS06770 0.37 -1.7 1,250,672 - MMJJ_RS06770 0.37 +0.3 1,250,672 - MMJJ_RS06770 0.37 +1.1 1,250,673 + MMJJ_RS06770 0.37 -2.7 1,250,673 + MMJJ_RS06770 0.37 -0.4 1,250,673 + MMJJ_RS06770 0.37 -0.1 1,250,673 + MMJJ_RS06770 0.37 +0.5 1,250,673 + MMJJ_RS06770 0.37 -0.0 1,250,678 - MMJJ_RS06770 0.37 +1.2 1,250,678 - MMJJ_RS06770 0.37 -0.3 1,250,678 - MMJJ_RS06770 0.37 +0.2 1,250,679 + MMJJ_RS06770 0.38 +0.4 1,250,702 + MMJJ_RS06770 0.40 +0.7 1,250,708 - MMJJ_RS06770 0.41 +1.0 1,250,711 - MMJJ_RS06770 0.41 -0.2 1,250,712 + MMJJ_RS06770 0.41 -0.0 1,250,712 + MMJJ_RS06770 0.41 +0.1 1,250,714 - MMJJ_RS06770 0.42 -0.2 1,250,715 + MMJJ_RS06770 0.42 -3.2 1,250,732 - MMJJ_RS06770 0.44 +1.6 1,250,733 + MMJJ_RS06770 0.44 +0.8 1,250,733 + MMJJ_RS06770 0.44 +0.2 1,250,803 - MMJJ_RS06770 0.52 +1.1 1,250,803 - MMJJ_RS06770 0.52 +0.8 1,250,815 + MMJJ_RS06770 0.54 +1.2 1,250,815 + MMJJ_RS06770 0.54 +0.6 1,250,887 + MMJJ_RS06770 0.62 -2.8 1,250,910 - MMJJ_RS06770 0.65 +1.2 1,250,910 - MMJJ_RS06770 0.65 +3.1 1,250,910 - MMJJ_RS06770 0.65 +0.1 1,250,910 - MMJJ_RS06770 0.65 -0.5 1,250,911 + MMJJ_RS06770 0.65 -0.8 1,250,921 + MMJJ_RS06770 0.67 +1.3 1,250,922 - MMJJ_RS06770 0.67 -0.8 1,250,922 - MMJJ_RS06770 0.67 -3.4 1,250,922 - MMJJ_RS06770 0.67 +0.5 1,250,922 - MMJJ_RS06770 0.67 +0.5 1,250,923 + MMJJ_RS06770 0.67 +0.1 1,250,923 + MMJJ_RS06770 0.67 +0.4 1,250,923 + MMJJ_RS06770 0.67 +1.2 1,250,933 - MMJJ_RS06770 0.68 -0.5 1,250,933 - MMJJ_RS06770 0.68 -0.7 1,250,934 + MMJJ_RS06770 0.68 +0.7 1,250,935 - MMJJ_RS06770 0.68 +0.4 1,250,935 - MMJJ_RS06770 0.68 -0.0 1,250,941 - MMJJ_RS06770 0.69 +0.5 1,250,954 - MMJJ_RS06770 0.71 +0.2 1,250,955 + MMJJ_RS06770 0.71 -0.2 1,251,002 + MMJJ_RS06770 0.76 -0.2 1,251,009 + MMJJ_RS06770 0.77 +1.6 1,251,009 + MMJJ_RS06770 0.77 +0.2 1,251,017 + MMJJ_RS06770 0.78 +0.7 1,251,091 - MMJJ_RS06770 0.87 +1.3 1,251,092 + MMJJ_RS06770 0.87 +1.0 1,251,095 - MMJJ_RS06770 0.87 -1.4 1,251,095 - MMJJ_RS06770 0.87 +0.9 1,251,096 + MMJJ_RS06770 0.88 -0.0 1,251,096 + MMJJ_RS06770 0.88 +0.9 1,251,133 - +0.4 1,251,135 - +1.4 1,251,135 - -0.1 1,251,135 - -0.0 1,251,136 + +0.4 1,251,170 + -0.6 1,251,170 + +0.5 1,251,170 + +0.5 1,251,170 + +0.2 1,251,179 - +0.3 1,251,192 + -1.0 1,251,203 - +0.2 1,251,204 + +0.6 1,251,206 + -0.4 1,251,206 + +0.4 1,251,214 - +0.2 1,251,215 + +0.3 1,251,237 - +0.9 1,251,241 - -0.3 1,252,006 - +0.2 1,252,171 + MMJJ_RS06775 0.30 -1.8 1,252,219 + MMJJ_RS06775 0.32 -0.4 1,252,470 - -1.2 1,253,831 - MMJJ_RS06775 0.84 -0.7 1,253,930 - MMJJ_RS06775 0.87 -2.0 1,254,422 + MMJJ_RS06780 0.11 +0.2 1,254,506 - MMJJ_RS06780 0.33 +1.2 1,254,799 - -0.7 1,254,823 - -0.7 1,254,851 - -1.3 1,254,851 - -2.5 1,254,971 - MMJJ_RS06785 0.23 -2.7 1,255,036 - MMJJ_RS06785 0.33 -5.0 1,255,075 - MMJJ_RS06785 0.39 -2.8 1,255,075 - MMJJ_RS06785 0.39 -4.2 1,255,104 - MMJJ_RS06785 0.43 -3.3 1,255,104 - MMJJ_RS06785 0.43 -0.3 1,255,104 - MMJJ_RS06785 0.43 -3.6 1,255,104 - MMJJ_RS06785 0.43 -2.8 1,255,104 - MMJJ_RS06785 0.43 -2.4 1,255,126 + MMJJ_RS06785 0.46 -1.4 1,255,142 - MMJJ_RS06785 0.49 -2.0 1,255,157 + MMJJ_RS06785 0.51 +0.3 1,255,181 - MMJJ_RS06785 0.54 -2.4 1,255,185 - MMJJ_RS06785 0.55 -1.0 1,255,186 + MMJJ_RS06785 0.55 -3.3 1,255,186 + MMJJ_RS06785 0.55 -2.2 1,255,186 + MMJJ_RS06785 0.55 -5.0 1,255,186 + MMJJ_RS06785 0.55 -2.1 1,255,191 - MMJJ_RS06785 0.56 -3.4 1,255,191 - MMJJ_RS06785 0.56 -2.7 1,255,196 - MMJJ_RS06785 0.57 -1.8 1,255,196 - MMJJ_RS06785 0.57 -3.4 1,255,196 - MMJJ_RS06785 0.57 -3.1 1,255,215 + MMJJ_RS06785 0.60 -3.4 1,255,222 + MMJJ_RS06785 0.61 -4.0 1,255,315 - MMJJ_RS06785 0.74 -4.4 1,255,315 - MMJJ_RS06785 0.74 -1.6
Or see this region's nucleotide sequence