Experiment: Deoxyribonucleic from herring sperm 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04845 and H281DRAFT_04846 are separated by 301 nucleotides H281DRAFT_04846 and H281DRAFT_04847 are separated by 139 nucleotides H281DRAFT_04847 and H281DRAFT_04848 are separated by 445 nucleotides
H281DRAFT_04845: H281DRAFT_04845 - dolichol-phosphate mannosyltransferase, at 286,691 to 287,821
_04845
H281DRAFT_04846: H281DRAFT_04846 - Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose), at 288,123 to 288,578
_04846
H281DRAFT_04847: H281DRAFT_04847 - mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)/mannose-6-phosphate isomerase, type 2 (EC 5.3.1.8), at 288,718 to 290,151
_04847
H281DRAFT_04848: H281DRAFT_04848 - hypothetical protein, at 290,597 to 291,442
_04848
Position (kb)
288
289
290
291 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 287.737 kb on + strand at 287.756 kb on - strand at 287.756 kb on - strand at 287.889 kb on + strand at 287.889 kb on + strand at 287.913 kb on + strand at 287.929 kb on + strand at 287.929 kb on + strand at 287.929 kb on + strand at 287.930 kb on - strand at 288.027 kb on + strand at 288.027 kb on + strand at 288.028 kb on - strand at 288.028 kb on - strand at 288.117 kb on + strand at 288.118 kb on - strand at 288.118 kb on - strand at 288.305 kb on - strand, within H281DRAFT_04846 at 288.324 kb on + strand, within H281DRAFT_04846 at 288.420 kb on - strand, within H281DRAFT_04846 at 288.423 kb on + strand, within H281DRAFT_04846 at 288.468 kb on - strand, within H281DRAFT_04846 at 288.518 kb on + strand, within H281DRAFT_04846 at 288.578 kb on + strand at 288.578 kb on + strand at 288.579 kb on - strand at 288.579 kb on - strand at 288.579 kb on - strand at 288.579 kb on - strand at 288.603 kb on + strand at 288.664 kb on - strand at 288.664 kb on - strand at 288.668 kb on + strand at 288.668 kb on + strand at 288.668 kb on + strand at 288.669 kb on - strand at 288.669 kb on - strand at 288.669 kb on - strand at 288.669 kb on - strand at 288.669 kb on - strand at 288.779 kb on + strand at 288.779 kb on + strand at 288.779 kb on + strand at 288.780 kb on - strand at 288.780 kb on - strand at 288.843 kb on + strand at 288.843 kb on + strand at 288.844 kb on - strand at 288.860 kb on + strand at 288.861 kb on - strand at 288.861 kb on - strand at 288.993 kb on + strand, within H281DRAFT_04847 at 289.037 kb on + strand, within H281DRAFT_04847 at 289.037 kb on + strand, within H281DRAFT_04847 at 289.037 kb on + strand, within H281DRAFT_04847 at 289.037 kb on + strand, within H281DRAFT_04847 at 289.038 kb on - strand, within H281DRAFT_04847 at 289.038 kb on - strand, within H281DRAFT_04847 at 289.056 kb on - strand, within H281DRAFT_04847 at 289.072 kb on + strand, within H281DRAFT_04847 at 289.073 kb on - strand, within H281DRAFT_04847 at 289.073 kb on - strand, within H281DRAFT_04847 at 289.207 kb on + strand, within H281DRAFT_04847 at 289.391 kb on + strand, within H281DRAFT_04847 at 289.392 kb on - strand, within H281DRAFT_04847 at 289.398 kb on + strand, within H281DRAFT_04847 at 289.399 kb on - strand, within H281DRAFT_04847 at 289.399 kb on - strand, within H281DRAFT_04847 at 289.399 kb on - strand, within H281DRAFT_04847 at 289.399 kb on - strand, within H281DRAFT_04847 at 289.508 kb on + strand, within H281DRAFT_04847 at 289.529 kb on + strand, within H281DRAFT_04847 at 289.571 kb on + strand, within H281DRAFT_04847 at 289.571 kb on + strand, within H281DRAFT_04847 at 289.571 kb on + strand, within H281DRAFT_04847 at 289.589 kb on + strand, within H281DRAFT_04847 at 289.589 kb on + strand, within H281DRAFT_04847 at 289.589 kb on + strand, within H281DRAFT_04847 at 289.590 kb on - strand, within H281DRAFT_04847 at 289.590 kb on - strand, within H281DRAFT_04847 at 289.677 kb on - strand, within H281DRAFT_04847 at 289.677 kb on - strand, within H281DRAFT_04847 at 289.682 kb on + strand, within H281DRAFT_04847 at 289.720 kb on - strand, within H281DRAFT_04847 at 289.864 kb on + strand, within H281DRAFT_04847 at 289.865 kb on - strand, within H281DRAFT_04847 at 289.897 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.908 kb on + strand, within H281DRAFT_04847 at 289.909 kb on - strand, within H281DRAFT_04847 at 290.034 kb on + strand at 290.034 kb on + strand at 290.136 kb on + strand at 290.137 kb on - strand at 290.137 kb on - strand at 290.137 kb on - strand at 290.137 kb on - strand at 290.137 kb on - strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.169 kb on - strand at 290.169 kb on - strand at 290.335 kb on + strand at 290.335 kb on + strand at 290.336 kb on - strand at 290.336 kb on - strand at 290.378 kb on - strand at 290.378 kb on - strand at 290.394 kb on + strand at 290.395 kb on - strand at 290.462 kb on + strand at 290.463 kb on - strand at 290.463 kb on - strand at 290.463 kb on - strand at 290.476 kb on + strand at 290.476 kb on + strand at 290.476 kb on + strand at 290.476 kb on + strand at 290.605 kb on + strand at 290.605 kb on + strand at 290.605 kb on + strand at 290.606 kb on - strand at 290.606 kb on - strand at 290.629 kb on + strand at 291.039 kb on - strand, within H281DRAFT_04848 at 291.135 kb on - strand, within H281DRAFT_04848 at 291.151 kb on - strand at 291.151 kb on - strand, within H281DRAFT_04848 at 291.151 kb on - strand, within H281DRAFT_04848
Per-strain Table
Position Strand Gene LocusTag Fraction Deoxyribonucleic from herring sperm 10 mg/mL (C) remove 287,737 + +1.9 287,756 - -1.2 287,756 - +1.7 287,889 + +1.8 287,889 + +1.6 287,913 + +0.1 287,929 + +2.4 287,929 + +1.6 287,929 + +1.0 287,930 - +0.4 288,027 + +2.0 288,027 + +2.0 288,028 - +0.3 288,028 - +0.6 288,117 + +2.2 288,118 - +0.2 288,118 - +1.3 288,305 - H281DRAFT_04846 0.40 +1.0 288,324 + H281DRAFT_04846 0.44 +2.7 288,420 - H281DRAFT_04846 0.65 +0.1 288,423 + H281DRAFT_04846 0.66 +1.8 288,468 - H281DRAFT_04846 0.76 +0.3 288,518 + H281DRAFT_04846 0.87 +3.5 288,578 + +1.2 288,578 + +1.8 288,579 - +0.5 288,579 - +1.8 288,579 - -0.3 288,579 - +1.3 288,603 + +2.7 288,664 - -1.4 288,664 - -0.5 288,668 + +0.4 288,668 + -0.1 288,668 + -0.1 288,669 - +0.9 288,669 - +0.7 288,669 - +0.4 288,669 - +0.1 288,669 - -0.1 288,779 + -0.4 288,779 + -0.1 288,779 + +0.3 288,780 - -0.8 288,780 - -0.7 288,843 + -0.3 288,843 + +2.2 288,844 - -0.6 288,860 + -0.2 288,861 - -0.0 288,861 - +0.0 288,993 + H281DRAFT_04847 0.19 +0.3 289,037 + H281DRAFT_04847 0.22 -0.9 289,037 + H281DRAFT_04847 0.22 +1.1 289,037 + H281DRAFT_04847 0.22 +0.4 289,037 + H281DRAFT_04847 0.22 -0.0 289,038 - H281DRAFT_04847 0.22 +1.3 289,038 - H281DRAFT_04847 0.22 +1.2 289,056 - H281DRAFT_04847 0.24 -0.3 289,072 + H281DRAFT_04847 0.25 +0.8 289,073 - H281DRAFT_04847 0.25 -0.2 289,073 - H281DRAFT_04847 0.25 -0.5 289,207 + H281DRAFT_04847 0.34 +0.8 289,391 + H281DRAFT_04847 0.47 +0.2 289,392 - H281DRAFT_04847 0.47 -1.5 289,398 + H281DRAFT_04847 0.47 +0.4 289,399 - H281DRAFT_04847 0.47 +0.1 289,399 - H281DRAFT_04847 0.47 +0.5 289,399 - H281DRAFT_04847 0.47 +0.9 289,399 - H281DRAFT_04847 0.47 +0.0 289,508 + H281DRAFT_04847 0.55 -0.1 289,529 + H281DRAFT_04847 0.57 -0.2 289,571 + H281DRAFT_04847 0.59 +1.2 289,571 + H281DRAFT_04847 0.59 -0.4 289,571 + H281DRAFT_04847 0.59 +0.1 289,589 + H281DRAFT_04847 0.61 +0.4 289,589 + H281DRAFT_04847 0.61 +0.9 289,589 + H281DRAFT_04847 0.61 -0.0 289,590 - H281DRAFT_04847 0.61 +0.4 289,590 - H281DRAFT_04847 0.61 +0.0 289,677 - H281DRAFT_04847 0.67 +0.2 289,677 - H281DRAFT_04847 0.67 +0.3 289,682 + H281DRAFT_04847 0.67 +1.2 289,720 - H281DRAFT_04847 0.70 +0.6 289,864 + H281DRAFT_04847 0.80 -0.1 289,865 - H281DRAFT_04847 0.80 +0.5 289,897 + H281DRAFT_04847 0.82 -0.2 289,908 + H281DRAFT_04847 0.83 +0.6 289,908 + H281DRAFT_04847 0.83 -0.9 289,908 + H281DRAFT_04847 0.83 +0.5 289,908 + H281DRAFT_04847 0.83 -0.4 289,908 + H281DRAFT_04847 0.83 +0.1 289,908 + H281DRAFT_04847 0.83 +0.1 289,908 + H281DRAFT_04847 0.83 +0.3 289,909 - H281DRAFT_04847 0.83 +0.5 290,034 + +0.6 290,034 + +1.4 290,136 + +0.5 290,137 - -1.8 290,137 - +1.9 290,137 - -0.0 290,137 - -1.4 290,137 - +0.6 290,140 + +0.4 290,140 + +0.1 290,141 - +0.6 290,141 - -0.0 290,141 - +0.6 290,141 - -0.2 290,141 - -0.6 290,169 - +0.7 290,169 - +0.6 290,335 + +0.7 290,335 + +0.0 290,336 - +0.4 290,336 - +1.7 290,378 - +0.5 290,378 - +0.6 290,394 + +0.2 290,395 - +2.4 290,462 + +0.7 290,463 - +0.4 290,463 - -0.6 290,463 - +0.2 290,476 + -1.4 290,476 + -0.3 290,476 + +0.3 290,476 + +0.9 290,605 + +2.1 290,605 + +3.5 290,605 + +0.9 290,606 - +4.1 290,606 - +4.7 290,629 + +1.4 291,039 - H281DRAFT_04848 0.52 +2.4 291,135 - H281DRAFT_04848 0.64 +2.8 291,151 - +3.1 291,151 - H281DRAFT_04848 0.65 +1.6 291,151 - H281DRAFT_04848 0.65 +1.4
Or see this region's nucleotide sequence