Experiment: Deoxyribonucleic from herring sperm 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02587 and H281DRAFT_02588 are separated by 355 nucleotides H281DRAFT_02588 and H281DRAFT_02589 are separated by 232 nucleotides H281DRAFT_02589 and H281DRAFT_02590 overlap by 41 nucleotides
H281DRAFT_02587: H281DRAFT_02587 - Phospholipase/lecithinase/hemolysin, at 161,419 to 162,438
_02587
H281DRAFT_02588: H281DRAFT_02588 - PRC-barrel domain-containing protein, at 162,794 to 163,216
_02588
H281DRAFT_02589: H281DRAFT_02589 - hypothetical protein, at 163,449 to 164,057
_02589
H281DRAFT_02590: H281DRAFT_02590 - Quinol monooxygenase YgiN, at 164,017 to 164,319
_02590
Position (kb) 162
163
164 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 161.863 kb on + strand, within H281DRAFT_02587 at 161.863 kb on + strand, within H281DRAFT_02587 at 162.264 kb on - strand, within H281DRAFT_02587 at 162.379 kb on + strand at 162.415 kb on + strand at 162.453 kb on - strand at 162.513 kb on - strand at 162.610 kb on + strand at 162.620 kb on + strand at 162.621 kb on - strand at 162.873 kb on - strand, within H281DRAFT_02588 at 162.873 kb on - strand, within H281DRAFT_02588 at 163.020 kb on + strand, within H281DRAFT_02588 at 163.030 kb on + strand, within H281DRAFT_02588 at 163.031 kb on - strand, within H281DRAFT_02588 at 163.336 kb on - strand at 163.920 kb on + strand, within H281DRAFT_02589 at 164.041 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Deoxyribonucleic from herring sperm 10 mg/mL (C) remove 161,863 + H281DRAFT_02587 0.44 +0.2 161,863 + H281DRAFT_02587 0.44 -0.3 162,264 - H281DRAFT_02587 0.83 +0.1 162,379 + -3.4 162,415 + -0.2 162,453 - -0.1 162,513 - -0.9 162,610 + -0.5 162,620 + -0.1 162,621 - -0.2 162,873 - H281DRAFT_02588 0.19 -0.7 162,873 - H281DRAFT_02588 0.19 -0.1 163,020 + H281DRAFT_02588 0.53 +0.5 163,030 + H281DRAFT_02588 0.56 +1.6 163,031 - H281DRAFT_02588 0.56 +0.3 163,336 - -0.6 163,920 + H281DRAFT_02589 0.77 -0.5 164,041 + +0.5
Or see this region's nucleotide sequence