Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3006 and Dsui_3007 are separated by 4 nucleotides Dsui_3007 and Dsui_3008 overlap by 1 nucleotides Dsui_3008 and Dsui_3009 are separated by 128 nucleotides Dsui_3009 and Dsui_3010 are separated by 21 nucleotides
Dsui_3006: Dsui_3006 - (NiFe) hydrogenase assembly chaperone, HybE family, at 3,220,352 to 3,220,969
_3006
Dsui_3007: Dsui_3007 - hydrogenase assembly chaperone HypC/HupF, at 3,220,974 to 3,221,261
_3007
Dsui_3008: Dsui_3008 - hydrogenase expression/formation protein, at 3,221,261 to 3,221,761
_3008
Dsui_3009: Dsui_3009 - Ni,Fe-hydrogenase I large subunit, at 3,221,890 to 3,223,602
_3009
Dsui_3010: Dsui_3010 - polysulfide reductase, at 3,223,624 to 3,224,781
_3010
Position (kb)
3221
3222
3223
3224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3221.107 kb on - strand, within Dsui_3007 at 3221.137 kb on + strand at 3221.246 kb on - strand at 3221.416 kb on - strand, within Dsui_3008 at 3221.445 kb on - strand, within Dsui_3008 at 3221.590 kb on - strand, within Dsui_3008 at 3221.616 kb on - strand, within Dsui_3008 at 3221.657 kb on + strand, within Dsui_3008 at 3221.700 kb on - strand at 3221.700 kb on - strand, within Dsui_3008 at 3221.707 kb on - strand, within Dsui_3008 at 3221.757 kb on - strand at 3221.953 kb on - strand at 3221.969 kb on - strand at 3222.079 kb on + strand at 3222.229 kb on - strand at 3222.361 kb on - strand, within Dsui_3009 at 3222.528 kb on + strand, within Dsui_3009 at 3222.585 kb on + strand at 3222.585 kb on + strand at 3222.662 kb on + strand at 3222.739 kb on - strand, within Dsui_3009 at 3222.768 kb on - strand, within Dsui_3009 at 3222.773 kb on - strand, within Dsui_3009 at 3222.827 kb on + strand at 3223.153 kb on - strand at 3223.243 kb on + strand at 3223.265 kb on + strand, within Dsui_3009 at 3223.273 kb on - strand at 3223.405 kb on - strand, within Dsui_3009 at 3223.631 kb on + strand at 3223.632 kb on + strand at 3223.821 kb on + strand at 3223.951 kb on + strand, within Dsui_3010 at 3224.158 kb on - strand, within Dsui_3010 at 3224.242 kb on - strand, within Dsui_3010 at 3224.293 kb on - strand at 3224.442 kb on + strand, within Dsui_3010 at 3224.469 kb on + strand, within Dsui_3010
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 3,221,107 - Dsui_3007 0.46 +1.0 3,221,137 + -1.9 3,221,246 - +0.6 3,221,416 - Dsui_3008 0.31 +0.7 3,221,445 - Dsui_3008 0.37 +0.7 3,221,590 - Dsui_3008 0.66 +0.1 3,221,616 - Dsui_3008 0.71 -0.5 3,221,657 + Dsui_3008 0.79 -1.2 3,221,700 - -2.7 3,221,700 - Dsui_3008 0.88 +0.4 3,221,707 - Dsui_3008 0.89 -0.1 3,221,757 - -1.1 3,221,953 - -0.7 3,221,969 - -0.7 3,222,079 + +0.9 3,222,229 - -3.2 3,222,361 - Dsui_3009 0.27 -0.6 3,222,528 + Dsui_3009 0.37 +1.9 3,222,585 + -0.3 3,222,585 + -0.1 3,222,662 + -0.7 3,222,739 - Dsui_3009 0.50 +0.6 3,222,768 - Dsui_3009 0.51 -1.3 3,222,773 - Dsui_3009 0.52 -1.5 3,222,827 + -2.9 3,223,153 - -1.1 3,223,243 + -1.3 3,223,265 + Dsui_3009 0.80 -2.4 3,223,273 - +0.9 3,223,405 - Dsui_3009 0.88 -2.9 3,223,631 + -2.1 3,223,632 + -0.1 3,223,821 + -2.8 3,223,951 + Dsui_3010 0.28 -1.9 3,224,158 - Dsui_3010 0.46 -2.1 3,224,242 - Dsui_3010 0.53 +0.8 3,224,293 - -0.7 3,224,442 + Dsui_3010 0.71 +1.1 3,224,469 + Dsui_3010 0.73 +0.9
Or see this region's nucleotide sequence