Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2733 and Dsui_2734 are separated by 137 nucleotides Dsui_2734 and Dsui_2735 are separated by 576 nucleotides Dsui_2735 and Dsui_2736 are separated by 51 nucleotides Dsui_2736 and Dsui_2737 are separated by 63 nucleotides
Dsui_2733: Dsui_2733 - bacterioferritin, at 2,888,130 to 2,888,603
_2733
Dsui_2734: Dsui_2734 - bacterioferritin-associated ferredoxin, at 2,888,741 to 2,888,947
_2734
Dsui_2735: Dsui_2735 - glycine cleavage system T protein, at 2,889,524 to 2,890,615
_2735
Dsui_2736: Dsui_2736 - glycine cleavage system H protein, at 2,890,667 to 2,891,053
_2736
Dsui_2737: Dsui_2737 - glycine dehydrogenase, decarboxylating, at 2,891,117 to 2,894,017
_2737
Position (kb)
2889
2890
2891 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2888.698 kb on - strand at 2888.815 kb on + strand, within Dsui_2734 at 2888.856 kb on - strand, within Dsui_2734 at 2888.856 kb on - strand, within Dsui_2734 at 2888.947 kb on - strand at 2888.964 kb on - strand at 2888.964 kb on - strand at 2889.161 kb on + strand at 2889.228 kb on + strand at 2889.310 kb on + strand at 2889.312 kb on + strand at 2889.377 kb on + strand at 2889.377 kb on + strand at 2889.408 kb on + strand at 2889.421 kb on + strand at 2889.422 kb on - strand at 2889.563 kb on + strand at 2889.702 kb on - strand at 2889.775 kb on + strand, within Dsui_2735 at 2889.788 kb on - strand at 2889.849 kb on + strand, within Dsui_2735 at 2889.889 kb on + strand, within Dsui_2735 at 2889.994 kb on + strand, within Dsui_2735 at 2890.008 kb on - strand, within Dsui_2735 at 2890.060 kb on - strand, within Dsui_2735 at 2890.164 kb on - strand at 2890.169 kb on - strand, within Dsui_2735 at 2890.233 kb on + strand, within Dsui_2735 at 2890.291 kb on + strand, within Dsui_2735 at 2890.291 kb on + strand, within Dsui_2735 at 2890.294 kb on - strand, within Dsui_2735 at 2890.306 kb on + strand, within Dsui_2735 at 2890.314 kb on - strand at 2890.377 kb on + strand, within Dsui_2735 at 2890.390 kb on + strand, within Dsui_2735 at 2890.397 kb on + strand, within Dsui_2735 at 2890.398 kb on - strand at 2890.473 kb on + strand, within Dsui_2735 at 2890.481 kb on - strand, within Dsui_2735 at 2890.481 kb on - strand, within Dsui_2735 at 2890.498 kb on - strand at 2890.563 kb on + strand at 2890.597 kb on + strand at 2890.604 kb on + strand at 2890.612 kb on - strand at 2890.614 kb on + strand at 2890.622 kb on - strand at 2890.622 kb on - strand at 2890.662 kb on + strand at 2890.675 kb on + strand at 2890.676 kb on + strand at 2890.762 kb on + strand, within Dsui_2736 at 2890.772 kb on + strand, within Dsui_2736 at 2890.780 kb on - strand at 2890.818 kb on - strand, within Dsui_2736 at 2890.818 kb on - strand at 2890.938 kb on + strand, within Dsui_2736 at 2890.959 kb on + strand, within Dsui_2736 at 2891.061 kb on + strand at 2891.066 kb on + strand at 2891.071 kb on + strand at 2891.116 kb on - strand at 2891.119 kb on + strand at 2891.119 kb on + strand at 2891.321 kb on + strand at 2891.389 kb on + strand at 2891.415 kb on + strand, within Dsui_2737 at 2891.447 kb on + strand, within Dsui_2737 at 2891.451 kb on + strand, within Dsui_2737 at 2891.474 kb on - strand, within Dsui_2737 at 2891.544 kb on + strand, within Dsui_2737
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,888,698 - +0.6 2,888,815 + Dsui_2734 0.36 -1.0 2,888,856 - Dsui_2734 0.56 +0.6 2,888,856 - Dsui_2734 0.56 +1.4 2,888,947 - +1.4 2,888,964 - +0.8 2,888,964 - -0.1 2,889,161 + -1.1 2,889,228 + +1.7 2,889,310 + +1.5 2,889,312 + +1.3 2,889,377 + +0.9 2,889,377 + +1.4 2,889,408 + +1.3 2,889,421 + +1.2 2,889,422 - -0.0 2,889,563 + +0.3 2,889,702 - -1.8 2,889,775 + Dsui_2735 0.23 +0.8 2,889,788 - -2.6 2,889,849 + Dsui_2735 0.30 +0.9 2,889,889 + Dsui_2735 0.33 +0.1 2,889,994 + Dsui_2735 0.43 +1.3 2,890,008 - Dsui_2735 0.44 -3.3 2,890,060 - Dsui_2735 0.49 -2.4 2,890,164 - +0.8 2,890,169 - Dsui_2735 0.59 -0.8 2,890,233 + Dsui_2735 0.65 +1.0 2,890,291 + Dsui_2735 0.70 +0.8 2,890,291 + Dsui_2735 0.70 +0.4 2,890,294 - Dsui_2735 0.71 -2.2 2,890,306 + Dsui_2735 0.72 +0.1 2,890,314 - -2.1 2,890,377 + Dsui_2735 0.78 +1.0 2,890,390 + Dsui_2735 0.79 +1.2 2,890,397 + Dsui_2735 0.80 +1.0 2,890,398 - +0.2 2,890,473 + Dsui_2735 0.87 +2.2 2,890,481 - Dsui_2735 0.88 +0.1 2,890,481 - Dsui_2735 0.88 +0.1 2,890,498 - -1.1 2,890,563 + +1.1 2,890,597 + +1.4 2,890,604 + +1.0 2,890,612 - -2.4 2,890,614 + +1.2 2,890,622 - -1.4 2,890,622 - +1.5 2,890,662 + -1.2 2,890,675 + +0.7 2,890,676 + +0.6 2,890,762 + Dsui_2736 0.25 -0.4 2,890,772 + Dsui_2736 0.27 +0.2 2,890,780 - -2.4 2,890,818 - Dsui_2736 0.39 -0.9 2,890,818 - -0.4 2,890,938 + Dsui_2736 0.70 +1.2 2,890,959 + Dsui_2736 0.75 +0.8 2,891,061 + +0.9 2,891,066 + +0.5 2,891,071 + +1.4 2,891,116 - -2.1 2,891,119 + +1.2 2,891,119 + +1.7 2,891,321 + +1.4 2,891,389 + +1.0 2,891,415 + Dsui_2737 0.10 +0.9 2,891,447 + Dsui_2737 0.11 +0.4 2,891,451 + Dsui_2737 0.12 +0.2 2,891,474 - Dsui_2737 0.12 -2.1 2,891,544 + Dsui_2737 0.15 +1.0
Or see this region's nucleotide sequence