Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2575 and Dsui_2576 are separated by 13 nucleotides Dsui_2576 and Dsui_2577 overlap by 4 nucleotides
Dsui_2575: Dsui_2575 - hypothetical protein, at 2,725,416 to 2,726,186
_2575
Dsui_2576: Dsui_2576 - hypothetical protein, at 2,726,200 to 2,726,790
_2576
Dsui_2577: Dsui_2577 - type IV secretory pathway, VirB4 component, at 2,726,787 to 2,729,378
_2577
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2725.213 kb on - strand at 2725.242 kb on + strand at 2725.338 kb on + strand at 2725.346 kb on - strand at 2725.435 kb on + strand at 2725.444 kb on - strand at 2725.464 kb on - strand at 2725.537 kb on - strand, within Dsui_2575 at 2725.555 kb on + strand, within Dsui_2575 at 2725.560 kb on + strand, within Dsui_2575 at 2725.561 kb on + strand, within Dsui_2575 at 2725.563 kb on - strand, within Dsui_2575 at 2725.598 kb on + strand, within Dsui_2575 at 2725.628 kb on + strand, within Dsui_2575 at 2725.636 kb on - strand, within Dsui_2575 at 2725.665 kb on + strand, within Dsui_2575 at 2725.675 kb on + strand, within Dsui_2575 at 2725.762 kb on + strand, within Dsui_2575 at 2725.767 kb on + strand, within Dsui_2575 at 2725.805 kb on - strand, within Dsui_2575 at 2725.825 kb on + strand, within Dsui_2575 at 2725.869 kb on + strand, within Dsui_2575 at 2725.875 kb on - strand, within Dsui_2575 at 2725.905 kb on - strand, within Dsui_2575 at 2725.922 kb on - strand, within Dsui_2575 at 2726.195 kb on + strand at 2726.201 kb on + strand at 2726.254 kb on - strand at 2726.319 kb on - strand, within Dsui_2576 at 2726.390 kb on + strand at 2726.415 kb on - strand, within Dsui_2576 at 2726.416 kb on - strand, within Dsui_2576 at 2726.416 kb on - strand, within Dsui_2576 at 2726.439 kb on - strand, within Dsui_2576 at 2726.460 kb on + strand, within Dsui_2576 at 2726.598 kb on + strand, within Dsui_2576 at 2726.611 kb on - strand, within Dsui_2576 at 2726.658 kb on - strand, within Dsui_2576 at 2726.693 kb on - strand, within Dsui_2576 at 2726.700 kb on + strand, within Dsui_2576 at 2726.722 kb on + strand, within Dsui_2576 at 2726.883 kb on + strand at 2726.900 kb on + strand at 2726.986 kb on + strand at 2727.054 kb on - strand, within Dsui_2577 at 2727.054 kb on - strand, within Dsui_2577 at 2727.178 kb on + strand, within Dsui_2577 at 2727.208 kb on - strand, within Dsui_2577 at 2727.272 kb on - strand, within Dsui_2577 at 2727.472 kb on + strand, within Dsui_2577 at 2727.502 kb on + strand, within Dsui_2577 at 2727.556 kb on + strand, within Dsui_2577 at 2727.564 kb on - strand, within Dsui_2577 at 2727.587 kb on + strand, within Dsui_2577 at 2727.587 kb on + strand, within Dsui_2577 at 2727.587 kb on + strand, within Dsui_2577 at 2727.587 kb on + strand, within Dsui_2577 at 2727.595 kb on - strand, within Dsui_2577 at 2727.674 kb on + strand, within Dsui_2577 at 2727.674 kb on + strand, within Dsui_2577 at 2727.674 kb on + strand, within Dsui_2577 at 2727.679 kb on + strand, within Dsui_2577 at 2727.708 kb on + strand, within Dsui_2577
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,725,213 - -0.3 2,725,242 + +0.5 2,725,338 + -0.7 2,725,346 - -1.6 2,725,435 + +0.5 2,725,444 - +2.5 2,725,464 - -2.5 2,725,537 - Dsui_2575 0.16 -0.6 2,725,555 + Dsui_2575 0.18 -1.2 2,725,560 + Dsui_2575 0.19 +1.8 2,725,561 + Dsui_2575 0.19 +0.7 2,725,563 - Dsui_2575 0.19 -0.3 2,725,598 + Dsui_2575 0.24 +0.1 2,725,628 + Dsui_2575 0.27 -1.0 2,725,636 - Dsui_2575 0.29 -0.6 2,725,665 + Dsui_2575 0.32 -2.4 2,725,675 + Dsui_2575 0.34 +0.9 2,725,762 + Dsui_2575 0.45 -0.1 2,725,767 + Dsui_2575 0.46 -0.4 2,725,805 - Dsui_2575 0.50 +0.8 2,725,825 + Dsui_2575 0.53 -0.3 2,725,869 + Dsui_2575 0.59 +1.1 2,725,875 - Dsui_2575 0.60 -0.1 2,725,905 - Dsui_2575 0.63 -0.7 2,725,922 - Dsui_2575 0.66 -1.3 2,726,195 + +0.3 2,726,201 + -0.6 2,726,254 - -2.6 2,726,319 - Dsui_2576 0.20 -0.1 2,726,390 + -2.1 2,726,415 - Dsui_2576 0.36 -3.3 2,726,416 - Dsui_2576 0.37 +0.2 2,726,416 - Dsui_2576 0.37 -0.4 2,726,439 - Dsui_2576 0.40 -0.8 2,726,460 + Dsui_2576 0.44 -2.3 2,726,598 + Dsui_2576 0.67 -1.1 2,726,611 - Dsui_2576 0.70 -3.2 2,726,658 - Dsui_2576 0.77 -1.4 2,726,693 - Dsui_2576 0.83 -2.4 2,726,700 + Dsui_2576 0.85 -1.6 2,726,722 + Dsui_2576 0.88 +0.3 2,726,883 + -2.3 2,726,900 + -1.4 2,726,986 + -0.7 2,727,054 - Dsui_2577 0.10 +0.2 2,727,054 - Dsui_2577 0.10 +0.2 2,727,178 + Dsui_2577 0.15 -1.8 2,727,208 - Dsui_2577 0.16 -1.1 2,727,272 - Dsui_2577 0.19 +0.9 2,727,472 + Dsui_2577 0.26 -2.1 2,727,502 + Dsui_2577 0.28 -0.4 2,727,556 + Dsui_2577 0.30 -0.1 2,727,564 - Dsui_2577 0.30 -0.3 2,727,587 + Dsui_2577 0.31 -0.3 2,727,587 + Dsui_2577 0.31 -0.2 2,727,587 + Dsui_2577 0.31 +0.1 2,727,587 + Dsui_2577 0.31 -2.1 2,727,595 - Dsui_2577 0.31 -0.4 2,727,674 + Dsui_2577 0.34 +0.5 2,727,674 + Dsui_2577 0.34 +0.3 2,727,674 + Dsui_2577 0.34 +0.4 2,727,679 + Dsui_2577 0.34 -2.6 2,727,708 + Dsui_2577 0.36 -1.9
Or see this region's nucleotide sequence