Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2550 and Dsui_2551 are separated by 67 nucleotides Dsui_2551 and Dsui_2552 are separated by 172 nucleotides Dsui_2552 and Dsui_2553 overlap by 4 nucleotides Dsui_2553 and Dsui_2554 are separated by 127 nucleotides Dsui_2554 and Dsui_2555 overlap by 4 nucleotides Dsui_2555 and Dsui_2556 are separated by 87 nucleotides
Dsui_2550: Dsui_2550 - Protein of unknown function (DUF2726), at 2,703,131 to 2,703,694
_2550
Dsui_2551: Dsui_2551 - Helix-destabilising protein, at 2,703,762 to 2,704,055
_2551
Dsui_2552: Dsui_2552 - hypothetical protein, at 2,704,228 to 2,704,503
_2552
Dsui_2553: Dsui_2553 - Phage X family/Phage replication protein CRI, at 2,704,500 to 2,705,582
_2553
Dsui_2554: Dsui_2554 - hypothetical protein, at 2,705,710 to 2,705,973
_2554
Dsui_2555: Dsui_2555 - Bacteriophage coat protein B, at 2,705,970 to 2,706,167
_2555
Dsui_2556: Dsui_2556 - hypothetical protein, at 2,706,255 to 2,707,346
_2556
Position (kb)
2704
2705
2706 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2703.542 kb on - strand, within Dsui_2550 at 2703.605 kb on - strand, within Dsui_2550 at 2703.786 kb on + strand at 2703.794 kb on - strand, within Dsui_2551 at 2703.794 kb on - strand, within Dsui_2551 at 2703.794 kb on - strand, within Dsui_2551 at 2703.830 kb on + strand at 2703.838 kb on - strand, within Dsui_2551 at 2703.876 kb on - strand, within Dsui_2551 at 2703.946 kb on - strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.963 kb on - strand, within Dsui_2551 at 2703.963 kb on - strand, within Dsui_2551 at 2704.040 kb on + strand at 2704.048 kb on - strand at 2704.065 kb on - strand at 2704.065 kb on - strand at 2704.065 kb on - strand at 2704.145 kb on - strand at 2704.248 kb on + strand at 2704.606 kb on - strand at 2704.815 kb on + strand, within Dsui_2553 at 2704.855 kb on + strand, within Dsui_2553 at 2704.864 kb on - strand, within Dsui_2553 at 2704.869 kb on - strand, within Dsui_2553 at 2705.024 kb on - strand, within Dsui_2553 at 2705.077 kb on + strand, within Dsui_2553 at 2705.335 kb on + strand, within Dsui_2553 at 2705.527 kb on - strand at 2705.577 kb on + strand at 2705.680 kb on - strand at 2705.681 kb on - strand at 2705.753 kb on - strand, within Dsui_2554 at 2705.824 kb on + strand, within Dsui_2554 at 2705.894 kb on + strand, within Dsui_2554 at 2705.939 kb on + strand at 2706.007 kb on + strand, within Dsui_2555 at 2706.015 kb on - strand, within Dsui_2555 at 2706.043 kb on - strand, within Dsui_2555 at 2706.146 kb on - strand, within Dsui_2555 at 2706.170 kb on + strand at 2706.516 kb on - strand, within Dsui_2556 at 2706.542 kb on - strand, within Dsui_2556 at 2706.542 kb on - strand, within Dsui_2556 at 2706.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 2,703,542 - Dsui_2550 0.73 -1.7 2,703,605 - Dsui_2550 0.84 +1.5 2,703,786 + -0.5 2,703,794 - Dsui_2551 0.11 -0.8 2,703,794 - Dsui_2551 0.11 -0.9 2,703,794 - Dsui_2551 0.11 -0.4 2,703,830 + -1.6 2,703,838 - Dsui_2551 0.26 -0.3 2,703,876 - Dsui_2551 0.39 -0.0 2,703,946 - Dsui_2551 0.63 +0.5 2,703,955 + Dsui_2551 0.66 -1.8 2,703,955 + Dsui_2551 0.66 -2.4 2,703,955 + Dsui_2551 0.66 -0.8 2,703,963 - Dsui_2551 0.68 +0.9 2,703,963 - Dsui_2551 0.68 +0.3 2,704,040 + -1.1 2,704,048 - +2.4 2,704,065 - -2.4 2,704,065 - -3.1 2,704,065 - +0.5 2,704,145 - +0.1 2,704,248 + -0.8 2,704,606 - -1.8 2,704,815 + Dsui_2553 0.29 +1.2 2,704,855 + Dsui_2553 0.33 -1.8 2,704,864 - Dsui_2553 0.34 -2.5 2,704,869 - Dsui_2553 0.34 -1.4 2,705,024 - Dsui_2553 0.48 -1.5 2,705,077 + Dsui_2553 0.53 -2.6 2,705,335 + Dsui_2553 0.77 +0.2 2,705,527 - -2.6 2,705,577 + +3.2 2,705,680 - -1.8 2,705,681 - +0.2 2,705,753 - Dsui_2554 0.16 -1.9 2,705,824 + Dsui_2554 0.43 -1.5 2,705,894 + Dsui_2554 0.70 -0.6 2,705,939 + -0.4 2,706,007 + Dsui_2555 0.19 -1.8 2,706,015 - Dsui_2555 0.23 +1.0 2,706,043 - Dsui_2555 0.37 -0.7 2,706,146 - Dsui_2555 0.89 -0.4 2,706,170 + -0.5 2,706,516 - Dsui_2556 0.24 +1.2 2,706,542 - Dsui_2556 0.26 -0.8 2,706,542 - Dsui_2556 0.26 -1.9 2,706,542 - +0.2
Or see this region's nucleotide sequence