Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1801 and Dsui_1802 are separated by 121 nucleotides Dsui_1802 and Dsui_1803 are separated by 163 nucleotides Dsui_1803 and Dsui_1804 are separated by 237 nucleotides Dsui_1804 and Dsui_1805 are separated by 14 nucleotides Dsui_1805 and Dsui_1806 are separated by 228 nucleotides
Dsui_1801: Dsui_1801 - ATPase involved in chromosome partitioning, at 1,900,405 to 1,901,022
_1801
Dsui_1802: Dsui_1802 - tRNA-Met, at 1,901,144 to 1,901,220
_1802
Dsui_1803: Dsui_1803 - 16S ribosomal RNA, at 1,901,384 to 1,902,805
_1803
Dsui_1804: Dsui_1804 - tRNA-Ile, at 1,903,043 to 1,903,119
_1804
Dsui_1805: Dsui_1805 - tRNA-Ala, at 1,903,134 to 1,903,209
_1805
Dsui_1806: Dsui_1806 - 23S ribosomal RNA, at 1,903,438 to 1,906,319
_1806
Position (kb)
1901
1902
1903 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1900.396 kb on + strand at 1900.396 kb on + strand at 1900.399 kb on - strand at 1900.409 kb on + strand at 1900.428 kb on + strand at 1900.439 kb on + strand at 1900.456 kb on - strand at 1900.486 kb on + strand, within Dsui_1801 at 1900.486 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.499 kb on + strand, within Dsui_1801 at 1900.544 kb on + strand, within Dsui_1801 at 1900.573 kb on + strand, within Dsui_1801 at 1900.660 kb on + strand, within Dsui_1801 at 1900.667 kb on + strand, within Dsui_1801 at 1900.667 kb on + strand, within Dsui_1801 at 1900.667 kb on + strand, within Dsui_1801 at 1900.667 kb on + strand, within Dsui_1801 at 1900.667 kb on + strand, within Dsui_1801 at 1900.675 kb on - strand, within Dsui_1801 at 1900.675 kb on - strand, within Dsui_1801 at 1900.685 kb on + strand, within Dsui_1801 at 1900.704 kb on + strand, within Dsui_1801 at 1900.705 kb on - strand, within Dsui_1801 at 1900.761 kb on - strand, within Dsui_1801 at 1900.899 kb on + strand, within Dsui_1801 at 1900.906 kb on + strand, within Dsui_1801 at 1900.906 kb on + strand, within Dsui_1801 at 1900.914 kb on - strand, within Dsui_1801 at 1900.914 kb on - strand, within Dsui_1801 at 1900.914 kb on - strand, within Dsui_1801 at 1900.914 kb on - strand, within Dsui_1801 at 1900.916 kb on + strand, within Dsui_1801 at 1900.916 kb on + strand, within Dsui_1801 at 1900.923 kb on + strand, within Dsui_1801 at 1900.968 kb on + strand at 1900.979 kb on + strand at 1901.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,900,396 + +0.5 1,900,396 + +0.5 1,900,399 - -3.4 1,900,409 + -1.2 1,900,428 + +0.5 1,900,439 + +1.6 1,900,456 - -1.4 1,900,486 + Dsui_1801 0.13 +0.8 1,900,486 + Dsui_1801 0.13 +0.1 1,900,499 + Dsui_1801 0.15 -1.0 1,900,499 + Dsui_1801 0.15 +0.6 1,900,499 + Dsui_1801 0.15 +0.1 1,900,499 + Dsui_1801 0.15 +0.4 1,900,499 + Dsui_1801 0.15 +0.5 1,900,499 + Dsui_1801 0.15 -0.4 1,900,544 + Dsui_1801 0.22 +0.3 1,900,573 + Dsui_1801 0.27 +0.1 1,900,660 + Dsui_1801 0.41 -0.1 1,900,667 + Dsui_1801 0.42 +0.5 1,900,667 + Dsui_1801 0.42 +0.6 1,900,667 + Dsui_1801 0.42 +0.8 1,900,667 + Dsui_1801 0.42 +0.5 1,900,667 + Dsui_1801 0.42 -0.4 1,900,675 - Dsui_1801 0.44 -3.5 1,900,675 - Dsui_1801 0.44 -0.4 1,900,685 + Dsui_1801 0.45 -0.1 1,900,704 + Dsui_1801 0.48 -1.9 1,900,705 - Dsui_1801 0.49 +0.4 1,900,761 - Dsui_1801 0.58 -3.4 1,900,899 + Dsui_1801 0.80 +0.0 1,900,906 + Dsui_1801 0.81 +0.2 1,900,906 + Dsui_1801 0.81 +0.8 1,900,914 - Dsui_1801 0.82 -2.2 1,900,914 - Dsui_1801 0.82 -2.4 1,900,914 - Dsui_1801 0.82 -4.1 1,900,914 - Dsui_1801 0.82 +1.0 1,900,916 + Dsui_1801 0.83 +0.4 1,900,916 + Dsui_1801 0.83 +0.3 1,900,923 + Dsui_1801 0.84 +1.0 1,900,968 + -0.1 1,900,979 + -0.3 1,901,019 - -1.8
Or see this region's nucleotide sequence