Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1645 and Dsui_1646 are separated by 96 nucleotides Dsui_1646 and Dsui_1647 are separated by 151 nucleotides Dsui_1647 and Dsui_1648 are separated by 129 nucleotides Dsui_1648 and Dsui_1649 are separated by 19 nucleotides
Dsui_1645: Dsui_1645 - hemerythrin HHE cation binding domain-containing protein, at 1,726,784 to 1,727,362
_1645
Dsui_1646: Dsui_1646 - cAMP-binding protein, at 1,727,459 to 1,728,205
_1646
Dsui_1647: Dsui_1647 - oxygen-independent coproporphyrinogen III oxidase, at 1,728,357 to 1,729,754
_1647
Dsui_1648: Dsui_1648 - hypothetical protein, at 1,729,884 to 1,730,570
_1648
Dsui_1649: Dsui_1649 - universal stress protein UspA-like protein, at 1,730,590 to 1,731,018
_1649
Position (kb)
1728
1729
1730 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1727.357 kb on + strand at 1727.357 kb on + strand at 1727.357 kb on + strand at 1727.374 kb on + strand at 1727.374 kb on + strand at 1727.382 kb on - strand at 1727.382 kb on - strand at 1727.382 kb on - strand at 1727.382 kb on - strand at 1727.382 kb on - strand at 1727.382 kb on - strand at 1727.387 kb on - strand at 1727.419 kb on + strand at 1727.422 kb on + strand at 1727.467 kb on + strand at 1727.467 kb on + strand at 1727.471 kb on - strand at 1727.475 kb on + strand at 1727.619 kb on + strand at 1727.629 kb on + strand, within Dsui_1646 at 1727.726 kb on - strand, within Dsui_1646 at 1727.737 kb on - strand, within Dsui_1646 at 1727.924 kb on - strand, within Dsui_1646 at 1727.981 kb on - strand, within Dsui_1646 at 1728.005 kb on - strand, within Dsui_1646 at 1728.027 kb on + strand, within Dsui_1646 at 1728.096 kb on + strand, within Dsui_1646 at 1728.228 kb on - strand at 1728.235 kb on + strand at 1728.239 kb on + strand at 1728.271 kb on + strand at 1728.322 kb on + strand at 1728.489 kb on - strand at 1728.542 kb on + strand, within Dsui_1647 at 1728.557 kb on + strand, within Dsui_1647 at 1728.557 kb on + strand, within Dsui_1647 at 1728.647 kb on + strand, within Dsui_1647 at 1728.796 kb on + strand, within Dsui_1647 at 1728.844 kb on + strand, within Dsui_1647 at 1728.852 kb on - strand at 1728.867 kb on + strand, within Dsui_1647 at 1728.873 kb on + strand, within Dsui_1647 at 1729.072 kb on - strand at 1729.085 kb on + strand, within Dsui_1647 at 1729.313 kb on + strand, within Dsui_1647 at 1729.381 kb on - strand at 1729.384 kb on + strand, within Dsui_1647 at 1729.384 kb on + strand, within Dsui_1647 at 1729.405 kb on + strand, within Dsui_1647 at 1729.499 kb on + strand, within Dsui_1647 at 1729.559 kb on + strand, within Dsui_1647 at 1729.658 kb on + strand at 1729.797 kb on - strand at 1729.824 kb on - strand at 1729.849 kb on - strand at 1729.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,727,357 + +0.9 1,727,357 + +1.3 1,727,357 + -0.1 1,727,374 + +0.6 1,727,374 + -0.9 1,727,382 - -1.2 1,727,382 - +0.1 1,727,382 - +0.5 1,727,382 - -0.2 1,727,382 - +0.3 1,727,382 - -0.4 1,727,387 - +0.1 1,727,419 + +0.5 1,727,422 + +0.3 1,727,467 + -1.0 1,727,467 + -2.1 1,727,471 - +0.2 1,727,475 + -4.3 1,727,619 + -3.3 1,727,629 + Dsui_1646 0.23 -3.2 1,727,726 - Dsui_1646 0.36 -3.0 1,727,737 - Dsui_1646 0.37 -3.9 1,727,924 - Dsui_1646 0.62 -3.3 1,727,981 - Dsui_1646 0.70 -2.1 1,728,005 - Dsui_1646 0.73 -2.0 1,728,027 + Dsui_1646 0.76 -3.1 1,728,096 + Dsui_1646 0.85 -3.4 1,728,228 - +0.7 1,728,235 + -2.2 1,728,239 + -0.8 1,728,271 + -2.6 1,728,322 + -0.7 1,728,489 - -4.2 1,728,542 + Dsui_1647 0.13 -2.8 1,728,557 + Dsui_1647 0.14 -3.3 1,728,557 + Dsui_1647 0.14 -4.6 1,728,647 + Dsui_1647 0.21 -4.6 1,728,796 + Dsui_1647 0.31 -3.6 1,728,844 + Dsui_1647 0.35 -3.9 1,728,852 - -2.6 1,728,867 + Dsui_1647 0.36 -4.1 1,728,873 + Dsui_1647 0.37 -4.4 1,729,072 - -0.4 1,729,085 + Dsui_1647 0.52 -3.0 1,729,313 + Dsui_1647 0.68 -3.8 1,729,381 - -2.4 1,729,384 + Dsui_1647 0.73 -5.4 1,729,384 + Dsui_1647 0.73 -5.0 1,729,405 + Dsui_1647 0.75 -3.5 1,729,499 + Dsui_1647 0.82 -2.4 1,729,559 + Dsui_1647 0.86 -2.8 1,729,658 + -2.7 1,729,797 - -1.1 1,729,824 - -0.6 1,729,849 - -0.7 1,729,876 + +0.7
Or see this region's nucleotide sequence