Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0983 and Dsui_0984 are separated by 18 nucleotides Dsui_0984 and Dsui_0985 are separated by 62 nucleotides Dsui_0985 and Dsui_0986 overlap by 4 nucleotides Dsui_0986 and Dsui_0987 overlap by 1 nucleotides
Dsui_0983: Dsui_0983 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,010,975 to 1,013,275
_0983
Dsui_0984: Dsui_0984 - isopropylmalate/homocitrate/citramalate synthase, at 1,013,294 to 1,014,202
_0984
Dsui_0985: Dsui_0985 - putative Fe-S protein, at 1,014,265 to 1,014,480
_0985
Dsui_0986: Dsui_0986 - putative Fe-S protein, at 1,014,477 to 1,014,671
_0986
Dsui_0987: Dsui_0987 - Zn-dependent hydrolase, glyoxylase, at 1,014,671 to 1,015,606
_0987
Position (kb)
1014
1015 Strain fitness (log2 ratio)
-1
0
1
2 at 1013.311 kb on + strand at 1013.331 kb on + strand at 1013.382 kb on + strand at 1013.531 kb on - strand, within Dsui_0984 at 1013.531 kb on - strand, within Dsui_0984 at 1013.606 kb on + strand, within Dsui_0984 at 1013.640 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.825 kb on - strand, within Dsui_0984 at 1013.829 kb on - strand, within Dsui_0984 at 1013.837 kb on - strand at 1013.925 kb on + strand, within Dsui_0984 at 1013.944 kb on - strand, within Dsui_0984 at 1013.966 kb on - strand at 1014.067 kb on + strand, within Dsui_0984 at 1014.108 kb on + strand, within Dsui_0984 at 1014.262 kb on + strand at 1014.262 kb on + strand at 1014.274 kb on + strand at 1014.274 kb on + strand at 1014.311 kb on + strand, within Dsui_0985 at 1014.311 kb on + strand, within Dsui_0985 at 1014.668 kb on - strand at 1014.717 kb on - strand at 1014.801 kb on - strand at 1014.818 kb on + strand, within Dsui_0987 at 1014.818 kb on + strand, within Dsui_0987 at 1014.826 kb on - strand, within Dsui_0987 at 1014.845 kb on + strand, within Dsui_0987 at 1014.984 kb on + strand, within Dsui_0987 at 1015.049 kb on + strand, within Dsui_0987 at 1015.113 kb on + strand, within Dsui_0987 at 1015.205 kb on - strand at 1015.247 kb on - strand, within Dsui_0987 at 1015.379 kb on + strand, within Dsui_0987 at 1015.384 kb on + strand, within Dsui_0987 at 1015.401 kb on + strand, within Dsui_0987
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 1,013,311 + +0.2 1,013,331 + +1.7 1,013,382 + +1.1 1,013,531 - Dsui_0984 0.26 +0.0 1,013,531 - Dsui_0984 0.26 +0.3 1,013,606 + Dsui_0984 0.34 +0.5 1,013,640 - Dsui_0984 0.38 -0.9 1,013,713 - Dsui_0984 0.46 -0.6 1,013,713 - Dsui_0984 0.46 +0.4 1,013,825 - Dsui_0984 0.58 +0.2 1,013,829 - Dsui_0984 0.59 +1.1 1,013,837 - +2.2 1,013,925 + Dsui_0984 0.69 +0.1 1,013,944 - Dsui_0984 0.72 +0.6 1,013,966 - +0.1 1,014,067 + Dsui_0984 0.85 +0.4 1,014,108 + Dsui_0984 0.90 +0.7 1,014,262 + +1.1 1,014,262 + +0.9 1,014,274 + +0.9 1,014,274 + +2.3 1,014,311 + Dsui_0985 0.21 +1.1 1,014,311 + Dsui_0985 0.21 +0.8 1,014,668 - +1.2 1,014,717 - +0.4 1,014,801 - -1.1 1,014,818 + Dsui_0987 0.16 +1.7 1,014,818 + Dsui_0987 0.16 +1.2 1,014,826 - Dsui_0987 0.17 -0.4 1,014,845 + Dsui_0987 0.19 +0.9 1,014,984 + Dsui_0987 0.33 +0.7 1,015,049 + Dsui_0987 0.40 +1.0 1,015,113 + Dsui_0987 0.47 +0.6 1,015,205 - +0.5 1,015,247 - Dsui_0987 0.62 +0.7 1,015,379 + Dsui_0987 0.76 +1.0 1,015,384 + Dsui_0987 0.76 +1.4 1,015,401 + Dsui_0987 0.78 +1.2
Or see this region's nucleotide sequence