Experiment: T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0386 and Dsui_0387 are separated by 55 nucleotides Dsui_0387 and Dsui_0388 are separated by 88 nucleotides Dsui_0388 and Dsui_0389 are separated by 12 nucleotides
Dsui_0386: Dsui_0386 - thiol-disulfide isomerase-like thioredoxin, at 385,974 to 386,522
_0386
Dsui_0387: Dsui_0387 - 3-dehydroquinate dehydratase, type II, at 386,578 to 387,078
_0387
Dsui_0388: Dsui_0388 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 387,167 to 387,616
_0388
Dsui_0389: Dsui_0389 - acetyl-CoA carboxylase, biotin carboxylase subunit, at 387,629 to 388,996
_0389
Position (kb) 387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 386.170 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.178 kb on - strand at 386.180 kb on + strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.229 kb on - strand, within Dsui_0386 at 386.280 kb on - strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.303 kb on - strand, within Dsui_0386 at 386.428 kb on + strand, within Dsui_0386 at 386.461 kb on - strand, within Dsui_0386 at 386.570 kb on - strand at 386.575 kb on + strand at 386.613 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.134 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate remove 386,170 + Dsui_0386 0.36 +0.8 386,170 + Dsui_0386 0.36 -0.1 386,178 - +0.2 386,180 + Dsui_0386 0.38 +1.0 386,214 - Dsui_0386 0.44 +0.1 386,214 - Dsui_0386 0.44 +0.5 386,229 - Dsui_0386 0.46 -0.7 386,280 - Dsui_0386 0.56 -1.8 386,295 + Dsui_0386 0.58 +1.0 386,295 + Dsui_0386 0.58 +0.6 386,303 - Dsui_0386 0.60 +0.3 386,428 + Dsui_0386 0.83 +1.0 386,461 - Dsui_0386 0.89 -1.1 386,570 - +2.0 386,575 + -0.2 386,613 + -3.5 387,086 + +0.5 387,086 + +0.3 387,086 + -0.1 387,134 + +0.1
Or see this region's nucleotide sequence